Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method for Pouzolzia zeylanica (L.) Benn. var. microphylla (Wedd.) W. T. Wang flavonoid

A technology of Pueraria multibranches and total flavonoids, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, plant raw materials, etc., can solve the problems that have not been reported yet, and the chemical components of Pueraria multibranches are rare, and achieve relief. Resin adsorption interference, low cost, high purity effect

Inactive Publication Date: 2011-11-02
NANJING ZELANG AGRI DEV
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few reports on the chemical constituents of Pueraria twigs, especially the flavonoids extract of Pueraria twigs.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Pulverize the kudzu vines, take 1kg, heat extract with petroleum ether (boiling range: 60-90°C) for 1h, then use 50% ethanol solution for microwave extraction twice, the extraction temperature is 90°C, 1h each time, extract Concentrate the solution into an extract, add pH 2 hydrochloric acid aqueous solution to the extract, filter out the insoluble matter, dissolve the insoluble matter with an appropriate amount of ethanol solution, add 500g of HZ806 macroporous resin and stir evenly, evaporate the solvent, pack the column, and wash it with distilled water to the bottom The column solution was colorless, and then eluted with 75% ethanol solution, and the eluate rich in total flavonoids of Pueraria radiata was collected, concentrated and dried to obtain 7.8 g of product with a content of 65.2%.

Embodiment 2

[0015] Pulverize the kudzu vines, take 1kg, heat extract with petroleum ether (boiling range: 60-90°C) for 1 hour, then reflux extract with 70% ethanol solution for 3 times, the extraction temperature is 80°C, 2 hours each time, extract Concentrate the solution into an extract, add pH4 hydrochloric acid aqueous solution to the extract, filter out the insoluble matter, dissolve the insoluble matter with an appropriate amount of ethanol solution, add 500g AB-8 macroporous resin and stir evenly, evaporate the solvent, pack the column, and wash with distilled water first When the column solution was colorless, it was eluted with 75% ethanol solution, and the eluate rich in the total flavonoids of Pueraria radiata was collected, concentrated and dried to obtain 14.5 g of the product with a content of 65.8%.

Embodiment 3

[0017] Pulverize the kudzu vines, take 1kg, heat extract with petroleum ether (boiling range: 60-90°C) for 1 hour, then use 60% ethanol solution for microwave extraction twice, the extraction temperature is 55°C, 2 hours each time, extract Concentrate the liquid into an extract, add pH 3 sulfuric acid aqueous solution to the extract, filter out the insoluble matter, dissolve the insoluble matter with an appropriate amount of ethanol solution, add 500g LSA-21 macroporous resin and stir evenly, evaporate the solvent, pack the column, and wash with distilled water first When the column solution was colorless, it was eluted with 80% ethanol solution, and the eluate rich in the total flavonoids of Pueraria argentina was collected, concentrated and dried to obtain 7.7 g of the product with a content of 78.6%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method for Pouzolzia zeylanica (L.) Benn. var. microphylla (Wedd.) W. T. Wang flavonoids. The method comprises the following steps: using Pouzolzia zeylanica (L.) Benn. var. microphylla (Wedd.) W. T. Wang as a raw material, carrying out hot extraction with petroleum ether having a boiling range of 60 to 90 DEG C, carrying out extraction with an ethanol solution having a concentration of 50 to 70% twice to three times, condensing the extracts to form an extractum, adding acid water to disperse the extractum, carrying out filtering to obtain insoluble substances, and separating the insoluble substances with a macroporous resin to obtain Pouzolzia zeylanica (L.) Benn. var. microphylla (Wedd.) W. T. Wang flavonoids. The content of flavone in the obtained flavonoids is as much as 65 to 85%. The method provided in the invention has a simple process and produces a product with high purity.

Description

Technical field: [0001] The invention belongs to the technical field of extraction of natural medicines, and relates to a preparation method of total flavonoids of kudzu root. Background technique: [0002] Multi-branch Mishuige (Pouzolzia zeylanica (L.) Benn.var.microphylla (Wedd.) W.T.Wang) is the whole plant of Urticaceae Mizushuige plant, also known as Shizhu and Shizhuzi , domestically distributed in southeastern Yunnan, Guangxi, Guangdong, Fujian, southern Jiangxi, Taiwan and other places, and widely distributed in tropical Asia abroad. Nature and flavor are bitter, cold, enter lung meridian. It has the effect of detoxification and anti-inflammation, and is used to treat boils, sores, inflammation and swelling. Pueraria multibranches mainly contain flavonoids, alkaloids and saponins and other substances. [0003] At present, there are few reports on the chemical constituents of Pueraria twigs, especially the flavonoids extract of Pueraria twigs. Therefore, providin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/185
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV