Polymerase chain reaction (PCR) primer pair for identifying or assisting in identifying duck tissues and/or organs and application of PCR primer pair

Abstract

The invention discloses a polymerase chain reaction (PCR) primer pair for identifying or assisting in identifying duck tissues and / or organs and application of the PCR primer pair. The PCR primer pair for identifying or assisting in identifying the duck tissues and / or organs consists of two single-stranded deoxyribonucleic acids (DNAs), namely 1) a single-stranded DNA shown as a sequence 1 in a sequence table and a single-stranded DNA shown as a sequence 2 in the sequence table, and 2) a single-stranded DNA shown as a reverse complementary sequence of the sequence 1 in the sequence table and a single-stranded DNA shown as a reverse complementary sequence of the sequence 2 in the sequence table. The detection method is high in specificity, and the duck tissues and / or organs doped into cattle, sheep, pigs and chicken can be identified; the whole result is clear and has high credibility; and the method is short in detection time, and only 8 hours is needed from the extracting of genomes to the finishing of enzyme cutting.

Description

technical field [0001] The invention relates to a pair of PCR primers for identifying or assisting in identifying duck tissues and / or organs and an application thereof. Background technique [0002] At present, in the field of production and sales of raw meat and meat products in China, some unscrupulous traders use adulteration and other means to deceive consumers in order to make huge profits. For example, cheap duck meat is used to pretend to be more expensive beef and mutton. Therefore, a set of methods for quickly and accurately identifying meat types and being able to adapt to the identification of mixed meat products will undoubtedly provide strong technical support for law enforcement officers. [0003] The traditional identification methods of meat products are mainly based on protein level analysis, including electrophoresis, immunoassay, ELISA and other techniques. In the process of operation, it is first necessary to prepare antibodies of the corresponding spec...

AI Technical Summary

Problems solved by technology

This type of identification technology is greatly affected by the structure of the protein (the part that plays a major role is the antigenic determinant), and the problems that often occur in practical applications include: 1. The thermal processing process will change the structure of the protein (the antigenic determinant). Detect heat-processed meat; 2. Cross-reaction may occur in the detection of mixed meat, because the structure of proteins of different species may have a certain degree of similarity, and the sensitivity of the test depends on the specificity of the prepared antibody

At present, there are also reports on the identification of duck meat by PCR-RFLP method, which is amplified by universal primers of vertebrates and identified by restriction fragment length polymorphism analysis, but the technology is relatively complicated, and the intuition of observation is poor when judging

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