Acid-resistance heterotrophic bacterium strain Z3 used for bioleaching treatment of sludge and livestock and poultry manure

A technology of biological leaching and livestock and poultry manure, applied in the field of environmental engineering, to achieve the effects of reducing toxicity, long-term vitality, and promoting growth

Active Publication Date: 2011-11-23
南京贝克特环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in actual operation, acid-resistant heterotrop

Method used

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  • Acid-resistance heterotrophic bacterium strain Z3 used for bioleaching treatment of sludge and livestock and poultry manure
  • Acid-resistance heterotrophic bacterium strain Z3 used for bioleaching treatment of sludge and livestock and poultry manure
  • Acid-resistance heterotrophic bacterium strain Z3 used for bioleaching treatment of sludge and livestock and poultry manure

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Embodiment Construction

[0020] Examples of the present invention are described below.

[0021] 1. Isolation and expansion of strain Z3

[0022] Prepare PDA medium, ingredients: 200g potatoes, 20g sucrose or glucose, 1000mL water, natural pH, sterilized at 121°C for 30min. The samples for the isolation of acid-resistant heterotrophic bacteria were taken from the acidified pig manure (pH value around 2.0) after the bioleaching treatment of pig manure and manure completed previously. Use an inoculation loop to take a ring of acidified pig manure and manure liquid, and separate it by marking on a PDA plate. Incubate the plate inoculated with the acidified pig manure and manure suspension at 28°C for 3 to 4 days until colonies grow. Separate a representative single colony with good growth on the PDA plate for smearing and microscopic examination. If there is impurity, pick the colony and streak repeatedly until the pure bacteria of the target strain are obtained, and transfer the slant to save it for lat...

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Abstract

The invention relates to an acid-resistance heterotrophic bacterium strain Z3 used for bioleaching treatment of sludge and livestock and poultry manure and belongs to the technical field of environmental engineering. The bacteria are cylindrical single-celled bacteria, the arthrospore size of the bacteria is 6<-12>mu m *3<-6>mu m, the bacterial colony is diffused on a plane, the bacteria grow quickly, the bacteria are flat, high in center and flock-like, the liquid culturing the bacteria is turbid, the precipitate is loose, the reproductive mode of the bacteria is fissiparism, and the formed arthrospores are single ones or connected into a chain. The bacteria can grow normally in an extremely acidic environment in which the pH value is 2.5, the most suitable pH is 2.5 to 6, and according to morphological and physiological biochemical characters and molecular biological assay, the bacterial strain belongs to Galactomyces geotrichum and the collection number of the bacterial strain is CGMCC No.4298. The strain in a pig manure and urban sludge bioleaching environment can quickly use water soluble small molecular weight organic matters or simple organic acids as a carbon source and anenergy source, so the bioleaching efficiency is improved, the rate of removal of heavy metals from the pig manure and urban sludge reaches over 90 percent, and the dehydration rates for the pig manure and urban sludge are 48 and 80 percent respectively.

Description

1. Technical field [0001] The invention relates to an acid-resistant heterotrophic bacterium Z3 applied to the biological leaching treatment of sludge and livestock and poultry manure. It is a technology for bioleaching treatment using the interaction of heterotrophic bacteria and autotrophic bacteria, and belongs to environmental engineering. technology field. 2. Technical Background [0002] At present, my country produces more than 7 million tons (dry matter) of various types of municipal sludge every year, as well as a larger amount of organic solid waste such as industrial sludge and livestock manure. These solid organic wastes all have different concentrations of heavy metals, because urban sewage treatment plants often receive some industrial wastewater, and farms add some metal salts as additives to feed, which significantly increases the heavy metals in manure. In addition, these sludges or manures also have the problem of being difficult to dewater. For example, ...

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Application Information

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IPC IPC(8): C12N1/14C02F11/02C12R1/645
Inventor 周立祥周俊
Owner 南京贝克特环保科技有限公司
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