Method of converting glycerin for preparation of lactic acid

A technology of glycerol and lactic acid, applied in the field of lactic acid, can solve the problems of environmental pollution, high production cost and high energy consumption, and achieve the effect of reducing production cost and energy consumption

Inactive Publication Date: 2011-11-23
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method requires the use of noble metals as catalysts, and the production cost is high. In addition, a large amount of alkali needs to be used in the reaction process, which will pollute the environment.
[0006] 2. Microbial fermentation of glycerin to prepare lactic acid: Chinese patent 200810102824.4 discloses a method for microbial fermentation of glycerin to prepare lactic acid. The bacteria used in this method are Klebsiella, Escherichia coli or Enterococcus faecalis, all of which are conditionally induced. bacteria, there are hidden dangers in biological safety. In addition, this method needs to sterilize the fermentation medium, which consumes a lot of energy

Method used

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  • Method of converting glycerin for preparation of lactic acid
  • Method of converting glycerin for preparation of lactic acid
  • Method of converting glycerin for preparation of lactic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Isolation and purification of strains

[0026] Put 1g of fresh soil sample or 1ml of water sample into 100mL enrichment medium, enrich and cultivate for 48h, take 5ml of enrichment culture medium and transfer it into new enrichment medium, and transfer for 3 generations in turn. Take 1ml of the enriched culture solution, serially dilute it with a 10-fold gradient, and select an appropriate dilution (10 -4 , 10 -5 , 10 -6 ) bacterial suspension 0.5ml, mixed with the separation medium and poured onto the plate. After culturing for 48 hours at 37°C, pick the strain with a large transparent circle. Transfer the obtained strains into a liquid fermentation medium, and after static cultivation at 37°C for 72 hours, after the fermentation is complete, measure the concentration of lactic acid in the fermentation broth according to the following method:

[0027] The reaction product was qualitatively and quantitatively analyzed by high-performance liquid chromatography (A...

Embodiment 2

[0067] (1) Fermentation method: 250ml triangular flask, stand for fermentation.

[0068] (2) Strains: Lactobacillus pentosus R3-8CGMCC No.4726.

[0069] (3) Medium:

[0070] Seed medium (g / L): peptone 10, yeast extract 5, K 2 HPO 4 2. Sodium acetate 5, pure glycerin 20, pH 6.5, 0.1MPa sterilization for 20min.

[0071] Fermentation medium (g / L): peptone 10, yeast extract 6, K 2 HPO 4 1.5, sodium acetate 6, waste glycerol (glycerol content about 96% w / w) 80, CaCO 3 (Separate sterilization) 30, pH 6.5, 0.1MPa sterilization for 20min.

[0072] (4) Fermentation process:

[0073] Seed culture: Inoculate Lactobacillus pentosus into a seed medium containing 20g / l glycerol (250ml Erlenmeyer flask, liquid volume: 100ml), and culture it statically at 37°C for 8h.

[0074] Fermentation culture: transfer the seed solution to the fermentation medium (250ml Erlenmeyer flask, liquid volume 100ml) containing 80g / l waste glycerol according to the inoculum size of 6%, and culture it stat...

Embodiment 3

[0078] (1) Fermentation method: 5L mechanical stirring fermenter, batch fermentation with air.

[0079] (2) bacterial classification: with embodiment 2.

[0080] (3) Medium:

[0081] Seed medium (g / L): peptone 10, yeast extract 5, K 2 HPO 4 2. Sodium acetate 5, pure glycerin 20, pH 6.5, 0.1MPa sterilization for 20min.

[0082] Fermentation medium (g / L): peptone 10, yeast extract 6, K 2 HPO 4 1.5, sodium acetate 6, waste glycerol (glycerin content about 96% w / w) 80, pH 6.5, 0.1MPa sterilization for 20min.

[0083] (4) Fermentation process:

[0084] Seed culture: with embodiment 2.

[0085] Fermentation culture: Use a 5L fermenter with a liquid volume of 2L, control the fermentation temperature at 34°C, and automatically adjust the pH to 6.0 with a NaOH solution with a concentration of 7.5mol / L. l The fermentation medium of waste glycerin, the stirring speed is 250rpm, the air volume is 0.8vvm, and the fermentation time is 96h.

[0086] (5) Fermentation result:

[0087...

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Abstract

The invention discloses a method of converting glycerin for preparation of lactic acid, and relates to lactic acid. The invention provides a strain for preparation of lactic acid, namely, lactobacillus pentosus R3-8. The method comprises the following steps: inoculating lactobacillus pentosus R3-8 into a seed culture medium containing glycerin for culturing, adding seed liquid into a fermentationmedium for microaerobic fermentation, allowing air in during fermentation and carrying out stirring, adding waste glycerin in a manner of fed batch, adding simultaneously aqueous alkali with a concentration of 6 to 8 M in a manner of fed batch, controlling the pH value to be 5.0 to 7.0, measuring dry weight concentration of the strain and concentration of glycerin and lactic acid in the process of fermentation, stopping fed batch adding of waste glycerin when the dry weight concentration of the strain is lower than 1.5 g/l, and stopping fermenting. According to the invention, the fermentationmedium can be directly used for fermentation without sterilization; the stain used in the invention belongs to probiotics, and therefore there is no hidden danger in biological security; energy consumption and production cost are reduced.

Description

technical field [0001] The invention relates to lactic acid, in particular to a method for converting glycerin to prepare lactic acid. Background technique [0002] Lactic acid, scientific name is α-hydroxypropionic acid, molecular formula is C 2 h 5 OCOOH is an important and multipurpose bio-based platform compound, which is widely used in food, medicine, daily chemical industry and organic chemical industry, among which the most important industrial application is for the synthesis of degradable polymers - polymers. Lactic acid (PLA). This polymer material not only has good chemical inertness and easy processing, but also has good biocompatibility, and is widely used in the manufacture of artificial bones, surgical sutures, packaging materials, daily-use plastic products, agricultural mulching films, and home decorations and other items. At present, there are three main methods for the synthesis of lactic acid, namely chemical synthesis, enzymatic and microbial ferment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/225
Inventor 方柏山赵耿
Owner XIAMEN UNIV
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