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Method for producing lactic acid by using immobilized cells for converting glycerin

A technology for immobilizing cells and glycerol, applied in the field of lactic acid, can solve the problems of restricting the industrial application of lactic acid production, serious substrate inhibition, low production intensity, etc., and achieve the effects of saving purification and separation costs, low cost, and improving production intensity.

Active Publication Date: 2015-07-01
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using traditional fermentation techniques to directly ferment lactic acid with glycerol as a raw material often has problems such as severe substrate inhibition, low biomass, and low production intensity, which limits the industrial application of microbial conversion of glycerol to produce lactic acid.

Method used

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  • Method for producing lactic acid by using immobilized cells for converting glycerin
  • Method for producing lactic acid by using immobilized cells for converting glycerin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] What this embodiment provides is to carry out the process of batch fermentation of lactic acid by immobilized Lactobacillus pentosus in a fiber bed bioreactor with biodiesel by-product crude glycerol as raw material:

[0033] 1. Culture medium (the glycerol added in the medium is crude glycerol, a by-product of biodiesel, and the concentration of glycerol in the medium refers to the concentration of glycerol contained in the added crude glycerol):

[0034] 1) Seed medium ( / L):

[0035] Glycerin 40g, yeast powder 5g, tryptone 10g, sodium acetate 6g, K 2 HPO 4 1.5g, pH6.5;

[0036]2) Expanded culture medium ( / L): glycerol 40g, yeast powder 6g, tryptone 10g, sodium acetate 6g, K 2 HPO 4 1.5g, MnCl 2 ·H 2 O0.15g, defoamer 0.05g, pH6.0~6.2;

[0037] 3) Fermentation medium ( / L):

[0038] Glycerin 60g, yeast powder 6g, tryptone 10g, sodium acetate 6g, K 2 HPO 4 1.5g, MnCl 2 ·H 2 O0.15g, defoamer 0.05g, pH6.0~6.2;

[0039] 2. Strains: Lactobacillus pentosus R3-8; ...

Embodiment 2

[0050] What this embodiment provides is to carry out the process of feeding batch fermentation of lactic acid by immobilizing Lactobacillus pentosus in a fiber bed bioreactor with biodiesel by-product crude glycerol as raw material:

[0051] 1. Culture medium (the glycerol added in the medium is crude glycerol, a by-product of biodiesel, and the concentration of glycerol in the medium refers to the concentration of glycerol contained in the added crude glycerol):

[0052] 1) Seed medium ( / L):

[0053] Glycerin 40g, yeast powder 5g, tryptone 10g, sodium acetate 6g, K 2 HPO 4 1.5g, pH6.5;

[0054] 2) Expand culture medium ( / L):

[0055] Glycerin 40g, yeast powder 6g, tryptone 10g, sodium acetate 6g, K 2 HPO 4 1.5g, MnCl 2 ·H 2 O0.15g, defoamer 0.05g, pH6.0~6.2;

[0056] 3) Initial fermentation medium ( / L):

[0057] Glycerin 60g, yeast powder 6g, tryptone 10g, sodium acetate 6g, K 2 HPO 4 1.5g, MnCl 2 ·H 2 O0.15g, defoamer 0.05g, pH6.0~6.2;

[0058] 2. Strains: Lact...

Embodiment 3

[0068] What this embodiment provides is to carry out the process of repeatedly batch-fermenting lactic acid by immobilized Lactobacillus pentosus in a fiber bed bioreactor with biodiesel by-product crude glycerol as raw material:

[0069] 1. Culture medium (the glycerol added in the medium is crude glycerol, a by-product of biodiesel, and the concentration of glycerol in the medium refers to the concentration of glycerol contained in the added crude glycerol):

[0070] 1) Seed medium ( / L):

[0071] Glycerin 40g, yeast powder 5g, tryptone 10g, sodium acetate 6g, K 2 HPO 4 1.5g, pH6.5;

[0072] 2) Expand culture medium ( / L):

[0073] Glycerin 40g, yeast powder 6g, tryptone 10g, sodium acetate 6g, K 2 HPO 4 1.5g, MnCl 2 ·H 2 O0.15g, defoamer 0.05g, pH6.0~6.2;

[0074] 3) Fermentation medium ( / L):

[0075] Glycerin 60g, yeast powder 6g, tryptone 10g, sodium acetate 6g, K 2 HPO 4 1.5g, MnCl 2 ·H 2 O0.15g, defoamer 0.05g, pH6.0~6.2;

[0076] 2. Strains: Lactobacillus p...

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Abstract

The invention provides a method for producing lactic acid by using immobilized cells for converting glycerin, and relates to the lactic acid. The method comprises the following steps: inoculating a strain in a glycerin-containing seed culture medium and culturing so as to obtain a seed solution, inoculating the seed solution in a glycerin-containing enlarging culture solution for enlarging culture, feeding aqueous alkali so as to control the pH value of the enlarging culture solution to 4.5-7.5; when the concentration of thallus cells in the enlarging culture solution reaches 1.0-15.0g / L, pumping the enlarging culture solution into a reactor by using a pump; when the reactor is full filled with the enlarging culture solution, maintaining the circulation of the enlarging culture solution in a fermentation tank and the reactor; and when the concentration of free thallus cells is reduced to the bottom point, ending the immobilization process; and after completing the thallus cell immobilization, adding a glycerin-containing fermentation culture medium so as to control the concentration of initial glycerin to 5-200g / L, or directly replacing with the fermentation culture medium with the initial glycerin concentration of 5-200g / L, and carrying out immobilized fermentation so as to obtain the lactic acid.

Description

technical field [0001] The invention relates to lactic acid, in particular to a method for producing lactic acid by converting glycerol with immobilized cells. Background technique [0002] Lactic acid, scientific name - hydroxypropionic acid, can be divided into L-type, D-type and DL-type according to its optical activity. Lactic acid is a very important organic acid, which can be widely used in food, pharmaceutical, textile, printing and dyeing, cosmetics and other fields. Polylactic acid (PLA) synthesized from lactic acid is an organic polymer material with high strength, strong plasticity and environmental friendliness. High-quality PLA can be used for medical surgical sutures, bone fixation materials, and reinforcement materials for tissue defects. etc., have good biocompatibility; lower-quality polylactic acid can be used in agricultural greenhouses, disposable lunch boxes, etc., and can be degraded by microorganisms in the natural environment, and is considered to be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56C12N11/12C12R1/225
Inventor 方柏山严正平
Owner XIAMEN UNIV
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