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Preparation method of dihydroxyacetone by transforming glycerol

A technology of dihydroxyacetone and glycerol, which is applied in the field of converting glycerol to prepare dihydroxyacetone, which can solve the problems of long fermentation time, high culture conditions, and low production intensity, and achieve the effects of short fermentation time, high production intensity, and reduced production costs

Active Publication Date: 2014-09-03
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Chinese patent 201010271671 discloses a method for microbial fermentation of glycerol to prepare dihydroxyacetone. The strain used in this method is Gluconobacter oxydans, which has the disadvantages of low cell density, high culture conditions, long fermentation time and low production intensity.

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  • Preparation method of dihydroxyacetone by transforming glycerol
  • Preparation method of dihydroxyacetone by transforming glycerol
  • Preparation method of dihydroxyacetone by transforming glycerol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Isolation and purification of strains

[0024] Put 1g of fresh soil sample or 1ml of water sample into 100mL enrichment medium, enrich culture for 3-7 days, take 5ml of enrichment culture medium and transfer it to new enrichment medium, and transfer for 3 generations in turn. Take 1ml of the enriched culture solution, serially dilute it with a 10-fold gradient, and select an appropriate dilution (10 -4 , 10 -5 , 10 -6 ) bacterial suspension 0.5ml, mixed with the separation medium and poured onto the plate. After culturing for 48 hours at 30°C, a single colony was picked. Transfer the obtained bacterial colony into the liquid fermentation medium, shake and cultivate at 30°C for 30 hours, and then measure the concentration of dihydroxyacetone in the fermentation broth according to the following method:

[0025] Determination of dihydroxyacetone (DHA) by diphenylamine chromogenic method: put 0.500 g of the standard sample into a 100 mL volumetric flask, add water t...

Embodiment 2

[0040] (1) Fermentation method: 250ml Erlenmeyer flask, shaker fermentation.

[0041] (2) Strains: Flavobacterium halmsphilum ch20-1.

[0042] (3) Medium:

[0043] Seed medium (g / L): Chen Haihai 1000 (ml), peptone 5, yeast extract 5, K 2 HPO 4 2. Sorbitol 5, pH6.0, 0.1MPa sterilization for 20min.

[0044] Fermentation medium (g / L): aged sea water 1000 (ml), peptone 5, yeast extract 5, K 2 HPO 4 2. Waste glycerin 90, MgSO 4 ·7H 2 O 0.2, MnSO 4 ·H 2 O 0.3, pH 6.0, 0.1MPa sterilization for 20min.

[0045] (4) Fermentation process:

[0046] Seed culture: Inoculate Flavobacterium halophilus into the seed culture medium (250ml Erlenmeyer flask, liquid volume 50ml), and cultivate at 30°C for 16h.

[0047] Fermentation culture: transfer the seed solution to the fermentation medium (250ml Erlenmeyer flask, liquid volume 50ml) containing 90g / l waste glycerol according to the inoculum amount of 5%, and cultivate at 30°C for 30h.

[0048] (5) Fermentation result:

[0049] At ...

Embodiment 3

[0051] (1) Fermentation method: 5L mechanical stirring fermentation tank, batch fermentation with air

[0052] (2) bacterial classification: with embodiment 2

[0053] (3) Medium:

[0054] Seed medium (g / L): Chen Haihai 1000 (ml), peptone 5, yeast extract 5, K 2 HPO 4 2. Sorbitol 5, pH6.0, 0.1MPa sterilization for 20min.

[0055] Fermentation medium (g / L): aged sea water 1000 (ml), peptone 5, yeast extract 5, K 2 HPO 4 2. Waste glycerin 90, MgSO 4 ·7H 2 O 0.2, MnSO 4 ·H 2 O 0.3, pH 6.0, 0.1Mpa sterilization for 20min.

[0056] (4) Fermentation process:

[0057] Seed culture: with embodiment 2

[0058] Fermentation culture: Use a 5L fermenter with a liquid volume of 2L. The fermentation temperature is controlled at 30°C. The pH is automatically adjusted at 6.0 with a concentration of 3mol / L NaOH solution, and the seed solution is transferred to a 90g / l For the fermentation medium of waste glycerol, the stirring speed is 200rpm, the amount of air introduced is 6vvm, ...

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Abstract

A preparation method of dihydroxyacetone by transforming glycerol relates to a polyhydroxy ketose. Flavobacterium halmsphilum ch20-1 is inoculated into seed culture medium for cultivation. Then seed liquid is added into fermentation medium containing glycerol to perform aerobic fermentation. After NaOH solution feeding and fermentation, dihydroxyacetone is obtained. According to the invention, transforming of cheap material waste glycerol into biobased platform chemical dihydroxyacetone of high added value is accomplished by the use of microorganism and an outlet of effective utilization of waste glycerol is found. The method provided by the invention uses a strain with no hidden danger of biological safety. At the same time, it has a short fermentation time, high productivity, low bacterial culture condition and low production cost.

Description

technical field [0001] The invention relates to a polyhydroxy ketose, in particular to a method for converting glycerin to prepare dihydroxyacetone. Background technique [0002] Dihydroxyacetone, referred to as DHA, is the simplest polyhydroxy ketose, with the molecular formula C 3 h 2 o 3 , as an important chemical fine raw material is widely used in medicine, pesticides, coatings, dyes and pigments, flavors and fragrances, food additives, feed additives, daily chemicals, macromolecular monomers and polymers, chemical additives, organic chemical Raw materials and other industries, which are widely used in cosmetics, it can combine with free amino acids on the surface of the skin to form a fine film on the surface of the skin, reflect sunlight, shield ultraviolet rays, prevent excessive evaporation of water, and play a good role in moisturizing and Sun protection effect. At present, there are three main synthetic methods of dihydroxyacetone, namely chemical synthesis, e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/26C12R1/20
Inventor 方柏山陈喆王兆守
Owner XIAMEN UNIV
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