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Method for producing cellulase by adding sophorose lipid for inducing fermentation

A technology of cellulase and sophorolipids, which is applied in the field of fermentation to produce cellulase, can solve the problems of high price of sophorose and achieve remarkable effects, improved efficiency and high cellulase activity

Inactive Publication Date: 2011-11-23
天津实发中科百奥工业生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Sophorose has long been recognized as the most effective soluble inducer of synthetic cellulase, but sophorose is very expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Seed cultivation

[0026] The composition of the seed medium includes, unit 1L: cellulose 10g, yeast extract 10g, glycerin 10g, (NH 4 ) 2 SO 4 1.4 g, KH 2 PO 4 2g, CaCl 2 2H 2 O 0.4g, MgSO 4 ·7H 2 O 0.3 g, FeSO 4 ·7H 2 O 0.005 g, CoCl 2 ·6H 2 O 0.0037 g, MnSO 4 ·H 2 O 0.0016g, ZnSO 4 ·7H 2 O 0.0014 g, dilute to 1 L with distilled water, pH value 5.0; the strain used: Trichoderma reesei TJZKBIO956; first inoculate the cultivated Trichoderma reesei strain into the sterilized seed medium, and cultivate at 30°C , the cultivation time is 68h, and the seed solution is obtained;

[0027] (2) Sterilization of fermentation medium

[0028] After the fermentation medium was sterilized at 121°C for 20 minutes, the pH was 5.0, and cooled to 30°C; the composition of the fermentation medium included, unit 1L: cellulose 50g, yeast extract 10g, (NH 4 ) 2 SO 4 1.4 g, KH 2 PO 4 2g, CaCl 2 2H 2 O 0.4g, MgSO 4 ·7H 2 O 0.3 g, FeSO 4 ·7H 2 O 0.005 g, CoCl ...

Embodiment 2

[0038] (1) Seed cultivation

[0039]The composition of the seed medium includes, unit 1L: cellulose 10g, yeast extract 10g, glycerin 10g, (NH 4 ) 2 SO 4 1.4 g, KH 2 PO 4 2g, CaCl 2 2H 2 O 0.4g, MgSO 4 ·7H 2 O 0.3 g, FeSO 4 ·7H 2 O 0.005 g, CoCl 2 ·6H 2 O 0.0037 g, MnSO 4 ·H 2 O 0.0016g, ZnSO 4 ·7H 2 O 0.0014 g, dilute to 1 L with distilled water, pH value 5.0; the strain used: Trichoderma reesei TJZKBIO956; first inoculate the cultivated Trichoderma reesei strain into the sterilized seed medium, and cultivate at 30°C , the cultivation time is 68h, and the seed solution is obtained.

[0040] (2) Sterilization of fermentation medium

[0041] After the fermentation medium was sterilized at 121°C for 20 minutes, the pH was 5.0, and cooled to 30°C; the composition of the fermentation medium included, unit 1L: cellulose 50g, yeast extract 10g, (NH 4 ) 2 SO 4 1.4 g, KH 2 PO 4 2g, CaCl 2 2H 2 O 0.4g, MgSO 4 ·7H 2 O 0.3 g, FeSO 4 ·7H 2 O 0.005 g, CoCl 2...

Embodiment 3

[0052] (1) Seed cultivation

[0053] The composition of the seed medium includes, unit 1L: cellulose 10g, yeast extract 10g, glycerin 10g, (NH 4 ) 2 SO 4 1.4 g, KH 2 PO 4 2g, CaCl 2 2H 2 O 0.4g, MgSO 4 ·7H 2 O 0.3 g, FeSO 4 ·7H 2 O 0.005 g, CoCl 2 ·6H 2 O 0.0037 g, MnSO 4 ·H 2 O 0.0016g, ZnSO 4 ·7H 2 O 0.0014 g, dilute to 1 L with distilled water, pH value 5.0; the strain used: Trichoderma reesei TJZKBIO956; first inoculate the cultivated Trichoderma reesei strain into the sterilized seed medium, and cultivate at 28 °C , the cultivation time is 72h, and the seed solution is obtained.

[0054] (2) Sterilization of fermentation medium

[0055] After the fermentation medium was sterilized at 121°C for 20 minutes, the pH was 5.0, and cooled to 28°C; the composition of the fermentation medium included, unit 1L: cellulose 50g, yeast extract 10g, (NH 4 ) 2 SO 4 1.4 g, KH 2 PO 4 2g, CaCl 2 2H 2 O 0.4g, MgSO 4 ·7H 2 O 0.3 g, FeSO 4 ·7H 2 O 0.005 g, CoCl ...

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PUM

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Abstract

The invention relates to a method for producing cellulase by adding sophorose lipid for inducing fermentation. The method comprises the following implementation steps of: (1) seed culturing: inoculating a cultured trichoderma reesei strain into a sterilized seed culture medium and culturing at the 28-30 DEG C for 68-72 hours; (2) sterilizing a fermentation culture medium: sterilizing the fermentation culture medium at 121 DEG C for 20 minutes till the pH is 5.0, and cooling to 28-30 DEG C; (3) fermentation: transferring a seed liquid into a sterilized 5L fermentation tank with the liquid volume 3L at the inoculum concentration of 5-10 percent, controlling the fermentation temperature at 28-30 DEG C till the pH is 5.0, fermenting for 40 hours and sampling and testing the concentration of the strain every 2 hours; (4) sterilizing sophorose lipid; (5) inducing with the sophorose lipid; and (6) roughly extracting cellulase. According to the method for producing the cellulase by adding the sophorose lipid for inducing fermentation, the synthesis of cellulase is induced effectively, higher cellulase activity is achieved, and the production cost of cellulase is lowered.

Description

technical field [0001] The invention relates to a method for producing cellulase by fermentation, in particular to a method for inducing fermentation to produce cellulase by adding sophorolipids. Background technique [0002] Cellulase is a group of enzymes that can hydrolyze solid cellulose into glucose, which can be used as a substrate for the production of many fermentation products, including fuel ethanol, a renewable alternative resource. Large-scale and cheap production of cellulase is very important for economical lignocellulose refining process. Many substances such as cellulose, carboxymethyl cellulose, various cellulose hydrolysates, lactose, sophorose, galactose, etc. are considered as potential inducers of biosynthetic cellulase. Among them, lactose is currently considered the most economically feasible inducer for industrial cellulase production. [0003] Sophorose has long been recognized as the most effective soluble inducer of synthetic cellulase, but sopho...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12R1/885
Inventor 徐勇虎刘云李睿颖国华胡娅君韩慧王晓琼范婷
Owner 天津实发中科百奥工业生物技术有限公司
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