A method for separating and purifying crude product l-alpha-glycerophosphocholine

Abstract

The invention discloses a method for separating and purifying a crude product L-alpha-glycerophosphocholine. The method comprises the following steps: firstly adding the crude product L-alpha-glycerophosphocholine into absolute ethanol, stirring and dissolving at 50 DEG C, then adding activated alkaline aluminum oxide, stirring and decolorizing for 2 hours, carrying out centrifugal separation, then removing the lower-layer aluminum oxide solid phase, drying and concentrating the upper-layer solution phase until the solution phase becomes a viscous oily substance, then passing the concentrated solution phase through a silica gel chromatography column, collecting the eluate containing the L-alpha-glycerophosphocholine product, evaporating and concentrating the collected eluate to remove the solvent, and carrying out vacuum-drying to obtain a purified product. In the method disclosed by the invention, the product has purity up to 99.5% or above, the used aluminum oxide and absolute ethanol can be regenerated and reused, the cost is low, and the method is more suitable for large-scale industrial production.

Description

1. Technical field [0001] The invention relates to a method for separating and purifying crude product L-α-glycerophosphocholine. 2. Background technology [0002] L-α-glycerophosphocholine (L-α-GPC) is a naturally occurring water-soluble phospholipid metabolite in the body, which can pass through the blood-brain barrier and is a source of choline for the synthesis of acetylcholine and phospholecithin. L-α-GPC is a cholinesterase inhibitor, which can effectively prevent and treat cerebral ischemic stroke, memory loss and cognitive dysfunction caused by mild to moderate Alzheimer's disease, mild to moderate multiple It can improve the memory and concentration of young patients, and improve the brain health of elderly patients. It is a good medicine for liver protection and a prescription drug for treating senile dementia. [0003] Separation and purification of the crude product L-α-glycerophosphorylcholine to make it reach the pharmaceutical grade, which can be used in clin...

AI Technical Summary

Problems solved by technology

Both of the above two separation and purification methods crystallize L-α-glycerophosphorylcholine, which requires higher purity of the crude product of L-α-glycerophosphorylcholine, and its separation and purification are more complicated and costly

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