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A fluorescent targeting nanomaterial and its preparation method

A technology of nanomaterials and fluorescent materials, applied in the field of fluorescent targeting nanomaterials and its preparation, can solve problems such as unseen technical solutions, complex synthesis of copolymer carriers, difficulty in introducing active molecules, etc., to achieve uniform distribution, simple structure, and convenient synthetic effect

Inactive Publication Date: 2011-11-30
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the synthesis of the copolymer carrier is complicated, and it is difficult to introduce active molecules
[0007] After retrieving domestic and foreign patent documents, and extensively consulting domestic and foreign publications, there is no technical solution identical to the present invention

Method used

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  • A fluorescent targeting nanomaterial and its preparation method
  • A fluorescent targeting nanomaterial and its preparation method
  • A fluorescent targeting nanomaterial and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Preparation of azide poly-p-chloromethylstyrene carrier

[0030] (1) Dissolve 5g of poly-p-chloromethylstyrene in 50mL of DMSO, add 10g of sodium azide, and react at room temperature for 5 hours. Pour the above reaction solution into methanol to precipitate a white precipitate. Filter and wash the filter cake with water , and dried in vacuum to obtain azide poly-p-chloromethylstyrene carrier.

[0031] (2) Dissolve 2g of poly-p-chloromethylstyrene in 200mL of DMSO, add 20g of sodium azide, and react at 100°C for 2 hours. Pour the above reaction solution into methanol, and a white precipitate precipitates. Filter, wash and filter with water. The cake was vacuum-dried to obtain azide poly-p-chloromethylstyrene carrier.

[0032] (3) Dissolve 5g of poly-p-chloromethylstyrene in 100mL of DMSO, add 15g of sodium azide, and react at 50°C for 4 hours. Pour the above reaction solution into methanol, and a white precipitate precipitates. Filter, wash and filter with wa...

Embodiment 2

[0034] Example 2: Preparation of Targeted Fluorescent Polymers

[0035] (1) Get respectively 2.0g of folic acid, 1.5g of alkynyl coumarin, and 6.0g of azide poly(p-chloromethylstyrene) prepared in Example 1(1), dissolve them in 20mL DMSO, and react with stirring at room temperature for 3 hours, Then, the reaction solution was poured into methanol, and a yellow precipitate was precipitated, filtered, the filter cake was washed, and vacuum-dried to obtain the targeted fluorescent polymer folic acid-coumarin-poly-p-chloromethylstyrene.

[0036] (2) get folic acid 1.5g respectively, alkynyl rhodamine 3.0g, azide poly-p-chloromethylstyrene 10g prepared in embodiment 1 (2), be dissolved in 30mL DMSO, stir reaction at room temperature for 5 hours, then The reaction solution was poured into methanol, a yellow precipitate was precipitated, filtered, the filter cake was washed, and vacuum-dried to obtain the targeted fluorescent polymer folic acid-rhodamine-poly-p-chloromethylstyrene. ...

Embodiment 3

[0039] Example 3: Preparation of targeted fluorescent nanomaterials

[0040] (1) Dissolve the folic acid-coumarin-poly-p-chloromethylstyrene obtained in Example 2(1) in tetrahydrofuran, prepare 5mL of 5% tetrahydrofuran solution, slowly add 10mL of water dropwise, stir for 5 hours, and dialyze The tetrahydrofuran was removed, followed by ultrasonic dispersion to obtain stable dispersed nanomaterials.

[0041] (2) Dissolve the folic acid-rhodamine-poly-p-chloromethylstyrene obtained in Example 2(2) in tetrahydrofuran, prepare 10 mL of 1% tetrahydrofuran solution, slowly add 50 mL of water, stir for 10 hours, and dialyze to remove THF, followed by ultrasonic dispersion to obtain stable dispersed nanomaterials.

[0042] (3) Dissolve the folic acid-methylcyanine-poly-p-chloromethylstyrene obtained in Example 2(3) in tetrahydrofuran, prepare 30 mL of 10% tetrahydrofuran solution, slowly add 150 mL of water, stir for 1 hour, and dialyze The removal of THF followed by ultrasonic di...

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Abstract

A fluorescent targeting nanomaterial and a preparation method thereof. The preparation method is: use the polymer as the carrier of nanomaterials, and carry out azide modification on it, and then use the click chemical reaction to couple the targeting molecule folic acid and fluorescent tracer compounds to the polymer chain to obtain the fluorescent targeting coupling substances, and then ultrasonically dispersed to obtain stable targeted fluorescent nanomaterials. The targeted fluorescent nanomaterial obtained in the present invention is evenly distributed and can effectively reach the tumor site. Through the tracer effect of the fluorescent compound, the targeting effect and the occurrence of lesions can be intuitively and conveniently investigated during the experiment and detection process. The invention has the advantages that: the preparation method is simple; the fluorescent nano system is stable; the targeting molecule folic acid can improve the targeting of the fluorescent material.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a fluorescent targeting nanometer material and a preparation method thereof. Background technique [0002] In recent years, the targeted delivery system has become one of the important means for the treatment and diagnosis of cancer, which can improve the targeting of diagnostic reagents and reduce their aggregation at non-target sites. With the continuous development of tumor molecular level, a series of receptors have been found on the surface of tumor cells, which are closely related to tumor growth and proliferation and overexpressed in tumor tissues. These tumor-specific receptors provide targets for tumor therapy. Folate receptors are overexpressed in a variety of tumor tissues, while they are low or absent in normal tissues. [0003] Folic acid (Folic acid, FA) is a small molecular vitamin, the structural formula is as follows, it can be reduced t...

Claims

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Application Information

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IPC IPC(8): C09K11/06A61K49/00A61P35/00B82Y40/00
Inventor 侯洁白钢张奇白芳曹美荣
Owner NANKAI UNIV
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