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A kind of method extracting ursolic acid from loquat leaf

A technology of loquat leaves and ursolic acid, which is applied in the fields of steroids and organic chemistry, can solve the problems of low extraction rate and long extraction time of ursolic acid, and achieve the effect of improving utilization capacity and huge economic value

Active Publication Date: 2011-12-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods all have defects such as longer extraction time and lower extraction rate of ursolic acid

Method used

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  • A kind of method extracting ursolic acid from loquat leaf
  • A kind of method extracting ursolic acid from loquat leaf
  • A kind of method extracting ursolic acid from loquat leaf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Fresh loquat leaves were washed and dried to constant weight, crushed to obtain 1kg of powder, added 10L of water, 2MPa steam explosion treatment for 200s, added 6.5L of ethanol aqueous solution (ethanol: water = 9:1), mixed and extracted in a water bath at 70°C, The extraction time is 1.5h, and the extraction frequency is 3 times. Collect the extract for 3 times, take the supernatant after centrifugation, concentrate under reduced pressure for 2 hours, remove a certain volume of ethanol solution, treat with DM-130 macroporous resin, collect 90% ethanol water eluate, concentrate under reduced pressure to a certain volume, And adjust the pH to 2-5, crystallize with ethanol for 2-6 times, and obtain 6.9 g of ursolic acid crystals (purity>95%).

Embodiment 2

[0020] Fresh loquat leaves were washed and dried to constant weight, crushed to obtain 1kg of powder, added 8L of water, 0.5Mp steam explosion treatment for 800s, added 5L of ethanol (ethanol: water = 100:0), mixed, stirred and extracted in a water bath at 20°C, extracted The time is 3h, and the number of extractions is 2 times. Collect the extract twice, take the supernatant after centrifugation, concentrate under reduced pressure for 2 hours, remove a certain volume of ethanol solution, treat with D-101 macroporous resin, collect 70% ethanol water eluate, concentrate under reduced pressure to a certain volume, And adjust the pH to 2-5, and crystallize with ethanol 2-6 times to obtain 6.4 g of ursolic acid crystals (purity>95%).

Embodiment 3

[0022] Fresh loquat leaves were washed and dried to constant weight, crushed to obtain 1kg of powder, added 9L of water, 5Mp steam explosion treatment for 50s, added 10L of ethanol aqueous solution (ethanol: water = 6:4), mixed, stirred and extracted in a water bath at 50°C, extracted The time is 0.5h, and the number of extractions is 4 times. Collect the extracts for 4 times, take the supernatant after centrifugation, concentrate under reduced pressure for 2 hours, remove a certain volume of ethanol solution, treat with D-101 macroporous resin, collect 50% ethanol water eluate, concentrate under reduced pressure to a certain volume, And adjust the pH to 2-5, crystallize with ethanol for 2-6 times, and obtain 7.2 g of ursolic acid crystals (purity>95%).

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Abstract

The invention discloses a method for extracting ursolic acid from loquat leaves, relating to the field of food science and the technical field of natural products. In the method, the loquat leaves are used as raw materials and are subjected to drying, crushing, steam explosion, alcohol extraction, reduced pressure distillation and concentration, macroporous absorption resin enrichment and crystallization technologies to obtain ursolic acid crystals. The method has the advantages that: the ursolic acid is separated from substances such as protein, lignin, cellulose and the like in the loquat leaves so as to be easily extracted through the steam explosion treatment; selective separation is performed to obtain an ursolic acid rough product through a macroporous absorption resin purification process; and thus, the frequency of ethanol crystallization is reduced, the efficiency for preparing the ursolic acid is increased, and the method has a broader application prospect.

Description

technical field [0001] A method for extracting ursolic acid from loquat leaves relates to the fields of food science and separation and extraction of natural products. Background technique [0002] Loquat leaf is the dried leaf of Rosaceae plant loquat. Loquat leaf belongs to the lung and stomach meridian. It is a traditional medicinal plant. "Recorded, it is a traditional Chinese medicine for relieving cough and asthma. It has the effects of reducing phlegm, relieving cough, and harmonizing the stomach. It is mainly used in the clinical treatment of acute and chronic bronchitis and other respiratory diseases. China is the most important loquat producer in the world, with the cultivated area and output accounting for more than 2 / 3 of the world. The main producing areas are Jiangsu, Fujian and Zhejiang. Most of the loquat leaves are currently treated as garbage. The active substances in loquat leaves include volatile oils, triterpene acids, flavonoids, saponins, organic acid...

Claims

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Application Information

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IPC IPC(8): C07J63/00
Inventor 杨瑞金赵伟华霄吴梨
Owner JIANGNAN UNIV
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