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Preparation of Tieguanyin Tea Polysaccharide Extract and Its Application in Tobacco Sheets

A technology for Tieguanyin tea and tobacco sheet, which is applied in the field of preparation of Tieguanyin tea polysaccharide extract, can solve the problem that the production time of tobacco sheet is not very long, and can improve the lack of tobacco aroma, improve the quality of smoking food and sensory comfort, and the process optimized effect

Active Publication Date: 2011-12-14
澳华达香精(广州)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the production time of tobacco sheet in my country is not very long, the application of tea polysaccharide in tobacco sheet is still blank for the time being.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh 100 grams of commercially available Anxi Tieguanyin tea leaves, dry them at 45°C for 2 hours, crush them and pass through a 50-mesh sieve, add 300 grams of pure water, let stand at 4°C for 12 hours, add 500 grams of pure water, ℃, ultrasonic extraction at 50kHz for 3 times, 15 minutes each time, combine the extracts, pass through a 1000-mesh sieve, and collect the extracts.

[0030] The above extract was microfiltered with a fiber membrane with a pore size of 0.2 μm, and the filtrate was ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 30 KDa at 0.1 MPa and 25° C., and the retentate was collected.

[0031] Under the conditions of 0.35 g of protease and water bath at 45°C, the enzymatic hydrolysis of the retentate was carried out for 2 hours, and the protease was inactivated at 85°C for 15 minutes, and the enzymatic hydrolysis was collected.

[0032] Mix the above enzymatic solution into 5 times the volume of 95% food grade ethanol, l...

Embodiment 2

[0037] Weigh 100 grams of commercially available Anxi Tieguanyin tea leaves, dry at 50°C for 2 hours, crush and pass through a 100-mesh sieve, add 300 grams of 0.5% sodium carbonate aqueous solution, let stand at 4°C for 24 hours, add 500 grams of The same sodium carbonate aqueous solution was subjected to ultrasonic extraction at 50° C. and 100 kHz for 3 times, each time for 15 minutes, and the combined extracts were passed through a 1000-mesh sieve to collect the extracts.

[0038] The above extract was microfiltered with a fiber membrane with a pore size of 0.2 μm, and the filtrate was ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 20 KDa at 0.1 MPa and 25° C., and the retentate was collected.

[0039] Under the conditions of 0.25 g of protease, stirring at 300 rpm, and heating at 5 °C / min to 50 °C at a constant temperature, the enzymatic hydrolysis of the retentate solution was carried out for 2 hours, and the protease was inactivated at 8...

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Abstract

The invention relates to a preparation method for iron Buddha tea polysaccharide extract and application thereof in tobacco sheets. The method for preparing the iron Buddha tea polysaccharide extract comprises the following steps of: crushing iron Buddha tea leaves, soaking, performing ultrasonic extraction, performing ultrafiltration, performing enzymolysis, precipitating in alcohol, performing centrifugal separation, and preparing the iron Buddha tea polysaccharide extract. The application of the iron Buddha tea polysaccharide extract in a production process for the tobacco sheets can improve the suction quality and the comfort degree of sense organs of the tobacco sheets. The preparation method has the advantages that: the purity of iron Buddha tea polysaccharide is improved by using the ultrafiltration technology, and the problem that protein is difficult to remove in the extraction of common plant polysaccharide is solved safely and effectively by using the enzymolysis technology; and the method is optimized in process, less in loss, high in yield, safe and reliable. When the iron Buddha tea polysaccharide extract is applied in the production of the tobacco sheets, the suction quality and the comfort degree of the sense organs of the tobacco sheets are improved; and the iron Buddha tea polysaccharide extract has the actual production significance and value.

Description

technical field [0001] The present invention relates to a preparation method and application of Tieguanyin tea polysaccharide extract, in particular to a method for preparing Tieguanyin tea polysaccharide extract by using ultrafiltration technology and enzymatic hydrolysis technology and the application of the Tieguanyin tea polysaccharide extract in tobacco application in flakes. Applying the Tieguanyin tea polysaccharide extract prepared by this technology in the production process of the tobacco sheet can improve the smoking quality and sensory comfort of the tobacco sheet. Background technique [0002] Tea is a drink widely accepted by people, and has enjoyed the reputation of health preservation since ancient times. The medicinal value of tea has a long history. In ancient my country, the physiological and pharmacological functions of tea have been known in many ways. Among them, the chemical composition of tea has a health care function on the human body, which is the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12S3/02A24B3/14
Inventor 梁俪恩侯一品黄艳倪跃新林德洪许展伟崔海滨王学娟黎志坚
Owner 澳华达香精(广州)有限公司
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