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Biological composite enzyme

A biological compound enzyme and culture technology, applied in the biological field, can solve the problems of inhibiting soil transformation ability, increasing plant dependence on chemical fertilizers, and difficulty in fully exerting the effect of organic fertilizers.

Inactive Publication Date: 2014-06-11
青岛乐德沃生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the extensive use of chemical fertilizers in agricultural production, the dependence of plants on chemical fertilizers is increasing, which inhibits the transformation ability of soil, makes it difficult to fully exert the effect of organic fertilizers, and the soil structure is not suitable for improvement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Product composition formula of the present invention is as follows:

[0026] The biological complex enzyme product composition is: Bacillus subtilis 8.0×10 per gram of product 8 A, Aspergillus awamori 4.0×10 7 1, protease 200IU, β-glucanase 220IU, xylanase 100IU, cellulase 110IU, amylase 270IU.

[0027] The concrete production method step of product of the present invention is as follows:

[0028] Bacteria culture preparation:

[0029] Bacillus subtilis agent culture preparation: Bacillus subtilis agent preparation: transfer and cultivate Bacillus subtilis from the slope, transfer the seed liquid after step-by-step expansion into the fermenter, and centrifuge after fermentation to obtain wet bacteria and fermentation liquid; After the fermentation is completed, the fermented liquid is vacuum-concentrated to 50% of the original volume by low-temperature negative pressure to obtain a bacterial concentrated liquid. Add carrier: Add the mixed carrier to the concentrated...

Embodiment 2

[0061] Basically the same as embodiment 1

[0062] Product composition formula of the present invention is as follows:

[0063] The biological complex enzyme product composition is: Bacillus subtilis 4.0×10 per gram of product 8 A, Aspergillus awamori 4.0×10 7 1, protease 150IU, β-glucanase 250IU, xylanase 50IU, cellulase 100IU, amylase 250IU.

[0064] The concrete production method step of product of the present invention is as follows:

[0065] Bacteria culture preparation:

[0066] Bacillus subtilis agent culture preparation: Bacillus subtilis agent preparation: transfer and cultivate Bacillus subtilis from the slope, transfer the seed liquid after step-by-step expansion into the fermenter, and centrifuge after fermentation to obtain wet bacteria and fermentation liquid; After the fermentation is completed, the fermented liquid is vacuum-concentrated to 50% of the original volume by low-temperature negative pressure to obtain a bacterial concentrated liquid. Add carrie...

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Abstract

The invention discloses a biological composite enzyme, belongs to the field of biotechnology and particularly relates to a biological composite enzyme product. The product provided by the invention consists of product of culture of aspergillus awamori, lactobacillus plantarum preparation and bacillus subtilis preparation. Each gram of biological composite enzyme contains 4.0 to 9.5*10<8> bacillus subtilis, 4.0 to 8.5*10<7> aspergillus awamori, 150 to 200IU of protease, 200 to 250IU of beta-glucanase, 50 to 150 IU of xylanase, 100 to 120 IU of cellulose and 250 to 300 IU of amylase. When the biological composite enzyme is applied into soil, the soil looseness of an active layer can be promoted, the soil fertility can be improved, and the sound soil environment suitable for the roots of crops to grow. The conversion of the soil bio-enzyme has obvious effects in aspects of reducing agricultural production cost, reducing consumption of fertilizer, restoring ecological productivity of soil, effectively improving crop yield, and the like and can give full play to the efficacy of the organic matters of the soil.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to biological complex enzyme products. Background technique [0002] Biological complex enzymes are widely used in industries such as textiles, food processing, medicine, fermentation, tobacco aging, feed, etc., but it is the first time that they are used to improve soil and promote crop growth, and there are no data reports. [0003] The reasonable compatibility of unconventional extracellular complex enzymes and intracellular complex enzymes produced by industrial microorganisms can effectively promote the growth of other microorganisms, and has a positive effect on the production of most organisms and metabolites, and has a significant activation effect on microbial enzymes. The idea of ​​developing preparation products for the fermentation industry was proposed. [0004] At the same time, a compound enzyme preparation for soil microorganisms (soil biological compound enzymes) has b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/50C12N9/42C12N9/28C12N1/20C12N1/14C09K17/00C12R1/665C12R1/125C12R1/25C09K101/00
Inventor 崔绍军
Owner 青岛乐德沃生物科技有限公司
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