Preparation method of active peptide hydrolyzate for removing hydroxyl free radicals in human body and active peptide oral liquid

A peptide hydrolyzate, free radical technology, applied in the field of preparation of active peptide hydrolyzate, can solve problems such as viscosity, emulsifying force and water absorption effect, etc.

Inactive Publication Date: 2011-12-21
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantage of this enzymatic hydrolysis method is that other functions of the product such as viscosity, emulsifying power and water absorptio

Method used

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  • Preparation method of active peptide hydrolyzate for removing hydroxyl free radicals in human body and active peptide oral liquid
  • Preparation method of active peptide hydrolyzate for removing hydroxyl free radicals in human body and active peptide oral liquid
  • Preparation method of active peptide hydrolyzate for removing hydroxyl free radicals in human body and active peptide oral liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1, a kind of preparation method of the active peptide that scavenges hydroxyl free radical of human body, its steps are as follows:

[0096] (1) Cleaning and drying: Take marine fish scales, wash them until there are no sundries and blood stains, and dry the cleaned fish scales at 55°C until the water content is 10%;

[0097] (2) Acid-base desalination treatment: After desalting treatment with 2N hydrochloric acid and 10% NaOH solution with a concentration of 10% by mass, wash with water until the pH is 5.5 to obtain a mixture of water and fish scales, and the weight percentage of fish scales in the mixture is 5 %;

[0098] (3) Enzymatic hydrolysis: add the mixed enzyme of papain and pepsin to the mixture of water and fish scales, papain accounts for 60% of its total weight in the mixed enzyme, and the added mixed enzyme accounts for 4.5% of the mixture weight of water and fish scales. %; then hydrolyzed at 45°C and pH 5.5 for 1.8 hours to obtain an enzymati...

Embodiment 2

[0100] Embodiment 2, a kind of preparation method of the active peptide that scavenges the hydroxyl free radical of human body, its steps are as follows:

[0101] (1) Cleaning and drying: Take marine fish scales, wash them until there are no sundries and blood stains, and dry the cleaned fish scales at 65°C until the water content is 5%;

[0102] (2) Acid-base desalination treatment: After desalting treatment with 2N hydrochloric acid and 10% NaOH solution by mass percentage concentration, wash with water until the pH is 6.5 to obtain a mixture of water and fish scales, and the weight percentage of fish scales in the mixture is 7 %;

[0103] (3) Enzymatic hydrolysis: add the mixed enzyme of papain and pepsin to the mixture of water and fish scales, papain accounts for 70% of its total weight in the mixed enzyme, and the added mixed enzyme accounts for 5.5% of the mixture weight of water and fish scales. %; then hydrolyzed at 50°C and pH 6.5 for 2.2 hours to obtain an enzymati...

Embodiment 3

[0105] Embodiment 3, a kind of preparation method of the active peptide that scavenges hydroxyl free radical of human body, its steps are as follows:

[0106] (1) Cleaning and drying: take marine fish scales, wash them until there are no sundries and blood stains, and dry the cleaned fish scales at 60°C until the water content is 8%;

[0107] (2) Acid-base desalination treatment: After desalting treatment with 2N hydrochloric acid and 10% NaOH solution with a concentration of 10% by mass, wash with water until the pH is 6.0 to obtain a mixture of water and fish scales, and the weight percentage of fish scales in the mixture is 6 %;

[0108] (3) Enzyme hydrolysis: add the mixed enzyme of papain and pepsin in the mixture of water and fish scale, papain accounts for 65% of its gross weight in the mixed enzyme, and the added mixed enzyme accounts for 5.0% of the mixture weight of water and fish scale %; then hydrolyzed at 48°C and pH6.0 for 2.0 hours to obtain an enzymatic soluti...

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Abstract

The invention discloses a preparation method of an active peptide for eliminating hydroxyl free radicals in human body, which is characterized in that it uses marine fish scales as raw materials, after drying and acid-base desalination treatment, adding papain and pepsin, and then adding papain and pepsin at 45- Hydrolyze at 50°C and pH5.5-6.5 for 1.8-2.2 hours to obtain an enzymatic hydrolyzate; then inactivate the enzymatically hydrolyzed solution, and then perform centrifugation to remove precipitates to obtain an active peptide hydrolyzate. The invention also discloses an active peptide health-care oral liquid for eliminating hydroxyl free radicals in human body, which uses concentrated active peptide hydrolyzate, honey, white sugar and citric acid as raw materials. The hydrolysis process of the invention is simple, the extraction process effectively utilizes fish scales left over from seafood processing, and the preparation process is energy-saving and environment-friendly. And the formula of its deodorization and oral liquid has the advantages of simplicity, practicality and low cost, and is easy to popularize and apply in industrial production.

Description

technical field [0001] The invention relates to a preparation method of an active peptide hydrolyzate; in particular, a preparation method of an active peptide hydrolyzate for removing hydroxyl free radicals from a human body; the invention also relates to an active peptide oral liquid prepared from the aforementioned active peptide hydrolyzate. Background technique [0002] In recent years, with the increasing scale of farming and the continuous improvement of farming technology, the output of farmed fish has increased rapidly. China's aquatic product output has maintained a rapid growth momentum. In 2005, the aquatic product output reached about 49 million tons, accounting for 35% of the world's aquatic product output, ranking first in the world. With the development of my country's aquaculture industry, people pay more and more attention to aquatic product processing. A large amount of leftovers are produced during processing, accounting for 30% to 50% of the total weight...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23L1/325A23L33/00A23L17/00
Inventor 杜云建赵玉巧
Owner HUAIHAI INST OF TECH
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