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Bacillus velezensis and application thereof in tomato blight antagonism

A technology for tomato fusarium wilt and bacillus, applied in application, bacteria, fungicides and other directions, can solve problems such as yield reduction, achieve high application value, reduce pesticide usage, and increase crop yield.

Inactive Publication Date: 2013-02-13
新疆万康诚一肥业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, processed tomatoes are still affected by various diseases during the years of planting, among which tomato fusarium wilt is one of the more serious diseases, and in severe cases, it will cause a large area of ​​production reduction

Method used

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  • Bacillus velezensis and application thereof in tomato blight antagonism
  • Bacillus velezensis and application thereof in tomato blight antagonism
  • Bacillus velezensis and application thereof in tomato blight antagonism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Isolation, screening and identification of Bacillus velezensis CGMCC No. 4992

[0021] (1) Isolation and screening of antagonistic bacteria

[0022] Gradient dilution separation method: Weigh 10 g of tomato rhizosphere soil sample into a triangular flask filled with 90 mL sterile water and glass beads, shake for 20 minutes and then stand for 20-30 seconds, which is 10 -1 Diluent, then use gradient method to dilute to 10 -2 To 10 -6 Diluent. Take 0.1mL of diluent (10 -3 , 10 -4 , 10 -5 , 10 - 6 ) Were spread on LB medium and cultured at 37°C. After the isolates grew, the colonies grown on the plate were streaked, separated and purified, and finally streaked and stored for later use.

[0023] Plate confrontation method for screening antagonistic bacteria: The plate confrontation method is slightly modified to activate the tomato fusarium wilt pathogen on the PDA medium slope. After the hyphae grow, the punch plate is placed on another fresh PDA medium In the center o...

Embodiment 2

[0039] Example 2: Growth factor of Bacillus velezensis CGMCC No. 4992

[0040] See the following way, but the specific growth factor should be determined according to the characteristics of Bacillus velezensis CGMCC No. 4992 provided by the present invention.

[0041] The strain LYT-5 was connected to LB medium containing different salt (NaCl) concentration, antibiotic (ampicillin) concentration, and pH, and placed in a 30°C incubator for 14 hours. Then the strain LYT-5 was connected to the LB medium and placed in 4℃, 10℃, 15℃, 20℃, 25℃, 30℃, 35℃, 40℃, 45℃, 50℃, 55℃, 60 Cultivate in an incubator at ℃. The results are shown in Table 2.

[0042] From Table 3, the strain LYT-5 is the most suitable growth factor.

[0043] Table 2: Effects of temperature, pH, salt and antibiotics on the growth of strain LYT-5

[0044] Temperature(℃)

Embodiment 3

[0045] Example 3: The antagonistic ability of the fermentation broth of Bacillus velezensis CGMCC No. 4992 against tomato Fusarium wilt

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Abstract

The invention discloses a Bacillus velezensis CGMCC (China General Microbiological Culture Collection Center) No.4992 and application thereof in tomato blight antagonism bacteria. A farmland soil sample on which tomatoes are planted for years in Xinjiang Fukang is subjected to antagonism bacteria screening to obtain a strain with stronger bacteriostatic activity; the strain is further screened and domesticated and bred; and the obtained strain is subjected to morphological specificity and 16S rRNA (Ribose Nucleic Acid) sequencing and system development analysis. The antagonism of the fermentation broth of the strain has outstanding technical effect on solving the tomato blight antagonism bacteria and has wide application field.

Description

Invention field [0001] The present invention relates to the technical field of biological control. Specifically, the present invention relates to a technical field of Velez Bacillus and its application in tomato fusarium wilt antagonism. Background technique [0002] Tomato Fusarium Wilt is a soil-borne disease that seriously harms the quality and yield of vegetables. Tomatoes are infected by Fusarium wilt from the seedling stage to the adult stage. The pathogens overwinter in the soil, spread with irrigation water and soil, and cause serious losses to tomato production in severe cases. Pesticide-based control measures have not only severely damaged the soil ecological structure, but also caused great harm to people’s health and the environment. Compared with the environmental pollution and pesticide residue problems caused by the application of chemical pesticides, biological control The method has the advantages of safety, economy and efficiency. This research started from th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
Inventor 詹发强杨文琦侯新强侯敏杨蓉崔卫东龙宣杞张慧涛
Owner 新疆万康诚一肥业有限公司
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