Vesicle preparation

A technology of vesicles and vitamins, which is applied in the directions of antiviral agents, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., can solve the problems of undeveloped hepatic parenchymal cell delivery particles, etc.

Active Publication Date: 2014-07-30
CHUGAI PHARMA CO LTD +1
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no delivery particle optimal for delivery to hepatic parenchymal cells has been developed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vesicle preparation
  • Vesicle preparation
  • Vesicle preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0146] N-(α-trimethylammonium acetyl)-dioleyl-D-glutamic acid chloride (Mutual Pharmaceutical Co., Ltd., Japan; trade name: DC-3-18:1), dioleoylphosphatidyl Ethanolamine (NOF Corporation, Japan) and cholesterol (Wako Pure Chemical Industries, Ltd., Japan) were dissolved in an appropriate amount of chloroform at a mixing ratio of 40:30:30 (molar ratio), and the solvent was distilled off under reduced pressure. Dried to make a lipid mixture.

[0147] Dry with a desiccator until the solvent can be reliably removed, then add 9% sucrose solution to the lipid mixture, and indirectly irradiate with ultrasonic waves using an ultrasonic generator under heating at 65°C, thereby obtaining a total lipid concentration of 2.5mM liposome coarse dispersion. Next, in order to make the liposome particle size uniform, two filters with a pore size of 0.2 μm were stacked and loaded into an extruder, and extruded under heat and pressure at about 65° C. (extrusion filtration). Furthermore, the same ...

Embodiment 2

[0151] DL-α-tocopherol was dissolved in a ratio of 2.5 μmol to DL-α-tocopherol (Tokyo Chemical Co., Ltd., Japan) and 400 μL of ethanol, and a filtrate (tocopherol liquid) filtered through a 0.2 μm filter was prepared.

[0152] In a 50 mL vial, 10 mL of the liposome dispersion prepared in [Example 1] was added, and 400 μL of tocopherol solution (10.0% relative to the total number of lipid moles) was added, followed by vortex mixing for 20 seconds. Further, incubation was carried out at room temperature for 1 hour to obtain a liposome dispersion in which vitamin E was adsorbed. The average particle diameter is 136.7 nm.

Embodiment 3

[0154] DL-α-tocopherol was dissolved in a ratio of 3.75 μmol to DL-α-tocopherol (Tokyo Chemical Co., Ltd., Japan) and 400 μL of ethanol, and a filtrate (tocopherol liquid) filtered through a 0.2 μm filter was prepared.

[0155] Into a 50 mL vial, 10 mL of the liposome dispersion prepared in [Example 1] was added, and 400 μL of the tocopherol solution (15.0% relative to the total number of lipid moles) was added, followed by vortex mixing for 20 seconds. Further, incubation was carried out for 1 hour at room temperature to obtain a liposome dispersion in which vitamin E was adsorbed. The average particle diameter is 133.5 nm.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle sizeaaaaaaaaaa
particle sizeaaaaaaaaaa
Login to view more

Abstract

The present invention discovers the property of using serum components to transport vitamin E to hepatic parenchymal cells, and by making vitamin E adsorb or chemically bind to the surface of the vesicles, the physiologically active substances contained in the vesicles can be effectively and specifically transported to the liver parenchymal cells.

Description

technical field [0001] The invention relates to a vesicle (small cell body, vesicle) capable of effectively introducing physiologically active substances into hepatic parenchymal cells and making the physiologically active substances effectively act in the hepatic parenchymal cells and its application. Background technique [0002] The present invention relates to antiviral agents, chemotherapeutic agents, peptides, nucleotides, oligonucleotides, antisense nucleic acids, aptamers, decoys and their analogs, small nucleic acid molecules such as siRNA, miRNA, dsRNA or shRNA, etc. Physiologically active substances are effectively introduced into the hepatic parenchymal cells, and the vesicles that allow the physiologically active substances to effectively act in the hepatic parenchymal cells. [0003] Examples are known in which nucleic acids such as genes or physiologically active substances such as antitumor agents are introduced into hepatic parenchymal cells to achieve gene ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K31/136A61K31/17A61K31/198A61K31/255A61K31/282A61K31/337A61K31/395A61K31/407A61K31/4164A61K31/4745A61K31/475A61K31/505A61K31/506A61K31/675A61K31/7008A61K31/704A61K31/7048A61K31/7088A61K33/24A61K38/00A61K38/21A61K45/00A61K47/18A61K47/22A61P3/00A61P31/12A61P35/00
CPCA61K31/675A61K31/7048A61K31/7088A61K31/255A61K9/127A61K31/505A61K47/22A61K31/7008A61K31/395A61K31/506A61K31/136A61K47/18A61K31/4164A61K31/407A61K31/17A61K31/704A61K38/00A61K31/198A61K31/282A61K31/475A61K47/48815A61K31/337A61K47/6911A61P3/00A61P31/12A61P35/00
Inventor 小原道法中野善郎蜂须丽水谷幸雄须藤正幸
Owner CHUGAI PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap