A kind of extraction method of insect or arthropod secondary metabolite

A technology of secondary metabolites and arthropods, applied in the direction of arthropod-derived medical raw materials, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve problems that have not been seen yet, and achieve the goal of ensuring reliability and accuracy Effect

Inactive Publication Date: 2011-12-28
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there have been no reports on other more compounds similar to small molecules of insect sec

Method used

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  • A kind of extraction method of insect or arthropod secondary metabolite
  • A kind of extraction method of insect or arthropod secondary metabolite
  • A kind of extraction method of insect or arthropod secondary metabolite

Examples

Experimental program
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Effect test

Embodiment 1

[0024] A method for extracting secondary metabolites of insects or arthropods, comprising the following steps: soaking the dried Changbaishan red ant powder residue with dichloromethane (the soaking should be able to submerge the powder residue, and the soaking process can be assisted by ultrasonic waves) Twice (filter after the first immersion, soak the powder residue with new dichloromethane again), soak for 48 hours each time; then filter to obtain the extract (combine the two extracts, concentrate and dry to obtain CH 2 Cl 2 Extract, the CH 2 Cl 2The extract is the fat-soluble metabolites of insects, sealed, and stored in the refrigerator for later use) and powder residue, after the powder residue is dried, soak twice in methanol aqueous solution with a mass concentration of 70%, each time for 36 hours; combine the two soaks Alcohol extract and freeze-dried at -60°C for 24 hours to obtain the extract (the extract is the insect water-soluble metabolite extracted, recorded...

Embodiment 2

[0026] Replace Changbaishan red ant powder residue with Jiuxiang worm powder residue, other is the same as embodiment 1.

Embodiment 3

[0028] Replace Changbai Mountain red ant powder slag with cockroach powder slag, other is the same as embodiment 1.

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Abstract

The invention relates to a method for extracting secondary metabolites of insects or arthropods. The method comprises the following steps: immersing powder slag of insects of arthropods by dichloromethane for more than twice, wherein each immersion time is not less than 36 hours, then performing a solid-liquid separation; drying the obtained powder slag and immersing by a methanol aqueous solution for more than twice, wherein each immersion time is not less than 36 hours, then merging and immersing the obtained an alcohol extract and freeze drying to obtain an extract. The insect is selected from Formica aquilonia, Aspongopus chinensis Dallas, cockroach, mole cricket or maggot; the arthropod is selected from scorpion or spider. According to the invention, the obtained extract has different antibacterial degree to G+positive bacteria or G-negative bacteria, and is capable of separating and preparing a compound with bacteriostatic activity, the method for extracting secondary metabolites of insects or arthropods establishes a methodology foundation for chemical research of the insects which has important academic meaning and good application prospect.

Description

technical field [0001] The present invention relates to the field of extraction and processing of insects, in particular to a method for extracting secondary metabolites of insects or arthropods. + Positive bacteria or G - Negative bacteria have different degrees of antibacterial effect and can be used to prepare drugs with antibacterial activity. Background technique [0002] Insects, as the species with the largest variety and quantity on the earth, are undoubtedly a biological treasure house containing huge natural product resources. In ancient my country, there are many records of insects being used as medicine, such as the masterpieces of traditional Chinese medicine "Compendium of Materia Medica" and "Supplements to Compendium of Materia Medica". Records of medicine. According to an incomplete survey, the species of insects in my country has exceeded 100,000, and only 209 species have been found to have medicinal value, which is less than 2% of the total number of ins...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61P31/04A61K35/646
Inventor 尹卫平任运来刘普李国芝段文录姜亚玲宋政伟李文渊
Owner HENAN UNIV OF SCI & TECH
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