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A method for obtaining yeast strains tolerant to multiple inhibitors

A technology for yeast strains and inhibitors is applied in the field of obtaining yeast strains tolerant to multiple inhibitors, which can solve the problems of increased cost, obvious inhibition effect, environmental sensitivity of inhibitors, etc., to improve tolerance, promote industrial production, The effect of stable growth traits

Active Publication Date: 2011-12-28
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a traditional strain for ethanol production, Saccharomyces cerevisiae has the advantages of efficient substrate utilization, product specificity and robustness, but it is sensitive to the inhibitor environment and is significantly inhibited
Traditional detoxification steps will increase the cost of production, so obtaining natural tolerant yeast strains to achieve in situ detoxification and efficient ethanol fermentation is of great significance in the field of cellulosic ethanol research

Method used

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  • A method for obtaining yeast strains tolerant to multiple inhibitors
  • A method for obtaining yeast strains tolerant to multiple inhibitors
  • A method for obtaining yeast strains tolerant to multiple inhibitors

Examples

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Effect test

Embodiment 1

[0024] A method for obtaining yeast strains resistant to multiple inhibitors (using Angelica (SC) as the starting bacterium to obtain tolerant bacteria by subculture in a low-sugar medium containing cellulose hydrolyzate inhibitors), comprising the steps of:

[0025] 1. Preparation of seed medium and subculture medium

[0026] Seed medium: glucose 20g / L, peptone 20g / L, yeast powder 10g / L, sterilized at 121°C for 20min.

[0027] Subculture medium: glucose 20g / L, peptone 20g / L, yeast powder 10g / L, sterilized at 121°C for 20min, adding cellulose hydrolyzate inhibitors furfural, phenol and acetic acid before inoculation, so that the content of furfural was 1.3g / L L, the content of acetic acid is 5.3g / L, and the content of phenol is 0.5g / L.

[0028] 2. Subculture

[0029] Angel yeast was inoculated in 100mL seed medium and cultured at 30°C and 150rpm for 10h. then by OD 600 The initial cell concentration of =0.1 was inoculated in a fermenter containing 3L subculture medium, cul...

Embodiment 2

[0035] A method for obtaining yeast strains resistant to multiple inhibitors (taking Angelia yeast as the starting bacterium and utilizing subculture to obtain tolerant bacteria in a high-sugar medium containing cellulose hydrolyzate inhibitors), comprising the following steps:

[0036] 1. Preparation of seed medium and subculture medium

[0037] Seed medium: glucose 20g / L, peptone 20g / L, yeast powder 10g / L, sterilized at 121°C for 20min.

[0038] Subculture medium 1: Glucose 100g / L, peptone 20g / L, yeast powder 10g / L, sterilized at 121°C for 20min, adding cellulose hydrolyzate inhibitors furfural, phenol and acetic acid before inoculation, so that the content of furfural was 1.04g / L, the content of acetic acid is 4.24g / L, and the content of phenol is 0.4g / L.

[0039] Subculture medium 2: Glucose 100g / L, peptone 20g / L, yeast powder 10g / L, sterilized at 121°C for 20min, inhibitors of cellulose hydrolyzate inhibitors furfural, phenol and acetic acid were added before inoculatio...

Embodiment 3

[0049] A method for obtaining yeast strains resistant to multiple inhibitors (using Saccharomyces cerevisiae BY4741 as the starting bacterium to obtain tolerant bacteria by subculture in a low-sugar medium containing cellulose hydrolyzate inhibitors), comprising the following steps:

[0050] 1. Preparation of seed medium and subculture medium

[0051] Seed medium: glucose 20g / L, peptone 20g / L, yeast powder 10g / L, sterilized at 121°C for 20min.

[0052] Subculture medium: glucose 20g / L, peptone 20g / L, yeast powder 10g / L, sterilized at 121°C for 20min, before inoculation, add cellulose hydrolyzate inhibitors: furfural, phenol and acetic acid, so that the content of furfural is 1.04g / L, the content of acetic acid is 4.24g / L and the content of phenol is 0.4g / L.

[0053] 2. Subculture

[0054] Saccharomyces cerevisiae BY4741 was cultured in 250 mL of 100 mL seed medium at 30° C. and 150 rpm for 10 h. then by OD 600 The initial cell concentration of =0.1 was inoculated in a 250...

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Abstract

The invention discloses a method for obtaining a yeast strain with the tolerance on various inhibitors, which comprises the following steps of: inoculating an activated saccharomyces cerevisias strain into a subculturing medium containing a cellulosic hydrolysate inhibitor to culture; after separating cell growing to a stationary phase, inoculating the obtained strain into the same fresh subculturing medium containing the cellulosic hydrolysate inhibitor to culture; and obtaining the strain in the stationary phase, i.e. the yeast strain with the tolerance on the various inhibitors. The yeast strain with the tolerance on the various inhibitors, which is obtained by the method disclosed by the invention, has improved tolerance on the cellulosic hydrolysate inhibitor and has stable growth traits, so that the in situ detoxification of hydrolysate is realized. The method has important significance for promoting the industrial production of cellulose ethanol.

Description

technical field [0001] The present invention relates to a method for obtaining yeast strains tolerant to multiple inhibitors. Background technique [0002] Energy has always been a major issue that plagues the economic and social development of countries all over the world. With the scarcity of oil, the development of new alternative energy has become a hot spot for scholars from all over the world, and bioenergy has received extensive attention and research. As a renewable energy, bioethanol has the characteristics of low pollution, easy transportation and storage, and can compete with gasoline in price. It is the new energy most likely to replace petroleum, and at the same time, it can alleviate the energy crisis to a certain extent. Has great development prospects. It is a mature technology to use starch and sugar as fermentation materials to produce ethanol by microbial fermentation, but the high cost of raw materials limits its industrial application. Lignocellulosic ...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12R1/865
Inventor 元英进王昕李炳志丁明珠
Owner TIANJIN UNIV
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