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Kit for detecting alkaline phosphatase

A kit and phosphatase technology, applied in the field of medical testing and determination, can solve the problems of low accuracy and poor stability of sodium nitrophenyl phosphate, and achieve the effects of high accuracy, easy promotion and use, and avoiding self-hydrolysis

Active Publication Date: 2014-01-08
CHANGCHUN HUILI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the purpose of the present invention is to provide a method for detecting alkaline phosphatase in view of the problems of poor stability of sodium p-nitrophenylphosphate, high detection results and low accuracy in existing kits for detecting alkaline phosphatase. Enzyme Kit

Method used

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  • Kit for detecting alkaline phosphatase
  • Kit for detecting alkaline phosphatase

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Embodiment 1

[0025] Embodiment 1: A kit for detecting alkaline phosphatase according to the present invention.

[0026] The kit for detecting alkaline phosphatase described in the present invention is a liquid single reagent, including: 10mmol / L sodium p-nitrophenylphosphate, 1g / L trehalose, 120mmol / L pH10.0~10.5HCl-AMP buffer solution, 0.5 mmol / L MgCl 2 ·6H 2 O, 0.5mmol / L zinc sulfate and 1mmol / L HEDTA.

Embodiment 2

[0027] Embodiment 2: A kit for detecting alkaline phosphatase according to the present invention.

[0028] The kit for detecting alkaline phosphatase of the present invention is a double reagent, the first reagent includes 2g / L trehalose and 5mmol / L sodium p-nitrophenylphosphate, the second reagent includes 140mmol / L pH10.0~10.5HCl- AMP buffer, 1.5mmol / L MgCl·6H 2 O, 0.2mmol / L zinc sulfate and 0.5mmol / L HEDTA.

Embodiment 3

[0029] Embodiment 3: A kit for detecting alkaline phosphatase according to the present invention.

[0030] The kit for detecting alkaline phosphatase of the present invention is a liquid single reagent, including: 2g / L trehalose, 20mmol / L sodium p-nitrophenylphosphate, 100mmol / L pH10.0~10.5HCl-AMP buffer solution, 0.4 mmol / L MgCl 6H 2 O, 0.6mmol / L zinc sulfate and 2mmol / L HEDTA.

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Abstract

Relating to the technical field of medical detection and visible in an aforementioned technical scheme, the invention discloses a kit for detecting alkaline phosphatase, comprising PNPP-Na2 (p-nitrophenyl phosphate disodium salt) and trehalose, a buffer solution with a pH value of 10-10.5 and activators. Existence of trehalose can stabilize PNPP-Na2, avoids self hydrolysis of PNPP-Na2, and improve the stability of PNPP-Na2. Tests show that the kit of the invention has good stability and long storage life, and application of the kit in the invention for detection can generate detection results of high accuracy, good precision and wide linear range. Characterized by wide application range and convenient popularization and application, the kit provided in the invention can be applied to hospitals of all levels, health prevention departments as well as medical and biological research institutes for measuring the alkaline phosphatase content in serum.

Description

technical field [0001] The invention relates to the technical field of medical examination and determination, in particular to a kit for detecting alkaline phosphatase. Background technique [0002] Alkaline phosphatase exists in almost every tissue of the human body, among which the liver is the most followed by the kidney, bones, intestines, and placenta and other tissues. The alkaline phosphatase in normal human serum mainly comes from bone and is produced by bone formation. Therefore, the activity of alkaline phosphatase in the blood increases in patients with bone diseases, especially when new bones are formed or in children who are growing. When the liver is damaged or obstructed, alkaline phosphatase enters the blood through the lymphatic channel and hepatic sinusoids, so the activity of alkaline phosphatase in serum increases when hepatobiliary diseases, especially biliary tract obstruction. [0003] Therefore, the reasons for high alkaline phosphatase can be divide...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/42G01N33/52G01N21/31
Inventor 董理
Owner CHANGCHUN HUILI BIOTECH CO LTD
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