Hydrogel type cell delivery vehicle for wound healing, and preparation method thereof

A hydrogel and cell technology, applied in the field of hydrogel cell delivery carrier composition and its preparation, can solve the problems of difficult to achieve effect, inconvenient to use, easy to detach from wounds and the like

Inactive Publication Date: 2012-01-04
MODERN CELL & TISSUE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned patent uses one substance or mixes two substances, which is easy to separate from the wound site, is difficult to achieve its effect, and is inconvenient to use

Method used

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  • Hydrogel type cell delivery vehicle for wound healing, and preparation method thereof
  • Hydrogel type cell delivery vehicle for wound healing, and preparation method thereof
  • Hydrogel type cell delivery vehicle for wound healing, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 12 pmole of substance P and 100 mg of Pluronic F127 (BASF) were mixed into 50 μl of physiological saline to prepare a hydrogel. The prepared hydrogel was used after making a wound with a diameter of 8 mm on the back of a Balb / c nude mouse (male, 5 weeks old). The control group used physiological saline. On day 7 the wounds on the backs of the mice were compared visually. figure 2 It is the result of visual observation after using the hydrogel containing substance P for 7 days. The results of the control group (a) and the experimental group (b) were visually observed. Compared with the control group, the experimental group had a moisturizing effect and an effect of inhibiting wound contraction, and promoted wound healing.

[0040] 2g, 2.5g and 3g of Pluronic F127 were respectively dissolved in 10ml of physiological saline to prepare 20%, 25% and 30% hydrogels. A rheometer (CVO, BOHLIN Instruments) was used to observe the viscosity change of the hydrogel with temperat...

Embodiment 2

[0042] 1×10 6 bone marrow mesenchymal stem cells and 100 mg Pluronic F127 were mixed in 50 μl of bone marrow mesenchymal stem cells (MSC for short) culture medium (MSCGM) to prepare a hydrogel. The prepared hydrogel was used after making a wound with a diameter of 8 mm on the back of Balb / c nude mice (male, 5 weeks old). The control group used physiological saline. On the 6th day after use, the hydrogel of the same composition was used again, and on the 14th day after the first use, the wound on the back of the rat was compared with the naked eye and then histologically observed. image 3 It is a schematic diagram of the results of visual observation after using the hydrogel containing bone marrow mesenchymal stem cells for 14 days, wherein (a) is the control group, and (b) is the experimental group. Figure 4 It is a schematic diagram of the results of histological observation after using the hydrogel containing bone marrow mesenchymal stem cells for 14 days, wherein (a) is ...

Embodiment 3

[0044] Mix 5×10 in 50 μl skin cell culture medium (DMEM) 5 Skin cells (fibroblasts, keratinocytes, and pigment cells) and 100mg of Pluronic F127 were used to prepare hydrogels. The prepared hydrogel was used after making a wound with a diameter of 8 mm on the back of a Balb / c nude mouse (male, 5 weeks old). On the 7th day, the wounds on the backs of the mice were observed with naked eyes and then histologically observed. Figure 5 It is a schematic diagram of the results of visual observation after using the hydrogel containing skin cells for 7 days, wherein (a) is the control group, and (b) is the experimental group. Figure 6 It is a schematic diagram of the result of histological observation after using the hydrogel containing skin cells for 7 days, wherein (a) is the control group, and (b) is the experimental group. From the results of visual observation, it can be seen that the experimental group has a moisturizing effect and an effect of inhibiting wound contraction co...

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PUM

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Abstract

The present invention relates to a hydrogel type cell delivery vehicle composition for wound healing and to a preparation method thereof. More particularly, the present invention relates to a hydrogel type cell delivery vehicle composition in which non-ionic surfactants, growth factors or substance-P, human-derived cells, and the like are distributed in aqueous media, to a use thereof for wound healing, and to a preparation method thereof. The hydrogel type composition of the present invention appropriately delivers cells and / or substance-P to the wound part, and has moistening effects, effects of preventing contraction of the wound, and effects of protecting cells, and can be used in an easy and convenient manner. The cells in the composition are delivered to the wound part to effectively heal the wound when the composition of the present invention is applied to or injected into the wounded body part.

Description

technical field [0001] The present invention relates to a hydrogel cell delivery carrier composition for healing wounds and a preparation method thereof, more particularly to a hydrogel formed by dispersing non-ionic surfactants, growth factors or substance P, and human derived cells in an aqueous medium. Glue cell transport carrier composition and wound healing use thereof and preparation method thereof. Background technique [0002] For a long time, many people have conducted research on cell reconstruction when body tissues are damaged, such as tissue reconstruction through drugs, tissue reconstruction using cells, and so on. But one of the big questions is how to deliver these drugs and in what composition of cells to the damaged tissue. The method of delivering this drug and cells includes: simply using a solution state, or further using a biomaterial sheet, sponge, or non-woven form such as collagen, and also using a cellulose adhesive. [0003] Substance P (substanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/30
CPCA61K47/10A61K38/046A61K35/28A61K35/33A61K35/30A61K38/1825A61K38/1808A61K38/30A61K38/193A61K9/06A61K35/36A61K9/0014A61P17/02A61K38/39A61K47/30A61K47/36
Inventor 林世焕金侖映尹沼喜
Owner MODERN CELL & TISSUE TECH
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