Method for producing oyster hydrolyzate through microbial fermentation

A technology for microbial fermentation and oysters, which is applied in the fields of aquatic product processing and microbial biology, can solve the problems of increased production cost and high price of finished protease, and achieves the effects of low cost, reasonable design of process parameters and full utilization.

Inactive Publication Date: 2012-01-25
HUAIHAI INST OF TECH
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

The price of finished protease is more expensive, which increases the production cost

Method used

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  • Method for producing oyster hydrolyzate through microbial fermentation
  • Method for producing oyster hydrolyzate through microbial fermentation
  • Method for producing oyster hydrolyzate through microbial fermentation

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Embodiment 1

[0038] Embodiment 1, a kind of microbial fermentation prepares the method for oyster hydrolyzate, it adopts Aspergillus oryzae liquid fermentation method, and its specific steps are as follows:

[0039] (1) Preparation of oysters: buy fresh Pacific oysters, clean them up, take out the oyster meat, and homogenize with a homogenizer;

[0040] (2) Preparation of fermentation substrate: the homogenate was diluted with distilled water until the M / V of the protein concentration was 15%, the pH value was adjusted to 7.0 with 1M NaOH, and pasteurized at 60°C for 30 minutes to obtain the fermentation substrate;

[0041] (3) Microbial fermentation: Activate the Aspergillus oryzae strain on the PDA slant medium, pick spores from the activated strain preservation slant and inoculate it into the PDA liquid medium, and cultivate it at 30°C and 120rpm until the cell concentration reaches 107CFU / ml, to obtain the fermented strain seed liquid; press the V / V 5% inoculation amount to inoculate t...

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Abstract

The invention discloses a method for preparing an oyster hydrolyzate through microbial fermentation, which is characterized in that a pacific oyster is fermented under the action of Aspergillus oryzae to prepare an oyster hydrolyzate. The preparation process comprises the following steps: taking fresh oyster meat, cleaning, homogenizing, and inoculating with the Aspergillus oryzae for fermentation; and after the fermentation is finished, carrying out filtration sterilization, and concentrating the filtrate to obtain the oyster hydrolyzate. By adopting a liquid fermentation mode, the method has the advantages of reasonable design of process parameters, high operability, low cost and high total nitrogen extraction rate and glycogen extraction rate, avoids the use of finished proteases and amylases, can make full use of oyster resources, and has better industrial application potential.

Description

technical field [0001] The invention relates to a method for preparing oyster hydrolyzate by liquid fermenting oysters, and belongs to the technical fields of aquatic product processing and microbial biology. Background technique [0002] Oysters are delicious and nutritious, known as "milk in the sea", and are one of the first batch of health care products listed as medicine and food in my country. Oyster protein is as high as 50%, the content of 8 essential amino acids is relatively high, accounting for 40% of the total amino acid, and the content of histidine and arginine required by infants and young children is relatively high, so it is a high-quality protein. More importantly, oyster meat contains more than 3% taurine. Taurine has anti-inflammatory and detoxifying effects, protects the liver and gallbladder, lowers blood fat, promotes brain development in children, and calms the nerves and nourishes the brain. In addition, oyster meat also contains up to 22.41% of gl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/33A23L17/40
Inventor 房耀维王淑军刘姝吕明生焦豫良
Owner HUAIHAI INST OF TECH
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