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Immunizing conjugation test method

A combination test and conjugate technology, which is applied in the fields of medicine and biological detection, can solve problems such as difficult quantitative or semi-quantitative, unsatisfactory detection, and low detection sensitivity, and achieve the goal of lowering the technical threshold, improving reliability, and simplifying experimental steps Effect

Inactive Publication Date: 2012-02-08
吴佩珊
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, the methods commonly used in the detection of biology, food, medicine, medicine, animal husbandry, etc. are: (1) enzyme-linked immunosorbent assay, which is suitable for detection in the laboratory, and the detection time generally takes 1 hour to several hours. More samples can be detected each time; (2) Colloidal particle-labeled immunochromatography or diafiltration test paper test, which uses colloidal gold and colloidal selenium as markers, can be quickly detected inside and outside the laboratory, and generally only needs 10-20 minutes Minutes, a single sample can be detected each time, but the detection sensitivity is low, it is not easy to quantify or semi-quantify, and cannot meet the needs of many tests

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1, hCG immunoadsorption binding detection reagent, its preparation process is as follows: (1) anti-beta-hCG monoclonal antibody is labeled with colloidal gold, and purify; (2) anti-alpha-hCG monoclonal antibody or polyantibody is coated on strip (Insert the strip plate into the coating solution); (3) Assemble the reagents and set them aside. The detection process is: (1) insert the strip-coated plate into the sample to be tested for 20 minutes; (2) insert the colloidal gold-labeled antibody working solution for 20 minutes after washing the strip-coated plate; (3) insert the strip-coated It is measured and interpreted by the plate set colorimeter.

[0014] Comparison results between hCG immunosorbent binding detection reagent and other detection methods: hCG national standard product is used as the test product, and microplate enzyme-linked immunoassay is used, the minimum detection amount is not higher than 5mIU / ml, which can be semi-quantitative; gold standa...

Embodiment 2

[0015] Embodiment 2, hepatitis B surface antigen immunosorbent binding detection reagent, its production process is as follows: (1) use colloidal gold to label hepatitis B surface antigen monoclonal antibody I, and purify; (2) the monoclonal antibody of hepatitis B surface antigen Anti-II or polyantibody is coated on fixed positions on both sides of the strip polystyrene plate (insert the strip plate into the coating solution); (3) Assemble reagents, ready for use. The detection process is: (1) insert the strip-coated plate into the sample to be tested for 20 minutes; (2) insert the colloidal gold-labeled antibody working solution for 20 minutes after washing the strip-coated plate; (3) insert the strip-coated It is measured and interpreted by the plate set colorimeter.

[0016] Comparison of hepatitis B surface antigen immunosorbent binding detection reagents with other detection methods: using the standard product of China National Institute for the Control of Pharmaceutical...

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Abstract

The invention provides an immunizing conjugation test method, belonging to the technical field of medical and biologic detection. The method comprises the steps of: taking an adsorbing material of an ELISA (enzyme-linked immunosorbent assay) as a carrier, taking a colloidal micelle and / or a dye as a tracer, and measuring and interpreting by a simple apparatus and / or naked eyes by adopting simplified ELISA steps to correspondingly detect antibody, antigen or nucleic acid of disease, food, medicine and the like. By synthetically adopting a new adsorbing material, the tracer and the simple apparatus, the precision and the sensitivity of the whole test can be simultaneously guaranteed, the requirements of experiments with various types can be met, the reliability of a test result can be improved, the test steps can be simplified, the easiness of the interpretation can be increased, and the technological threshold of laymen for using a laboratory reagent can be reduced.

Description

technical field [0001] The invention belongs to the technical fields of medicine and biological detection, and in particular relates to a novel immune binding test method. Background technique [0002] At present, the methods commonly used in the detection of biology, food, medicine, medicine, animal husbandry, etc. are: (1) enzyme-linked immunosorbent assay, which is suitable for detection in the laboratory, and the detection time generally takes 1 hour to several hours. More samples can be detected each time; (2) Colloidal particle-labeled immunochromatography or diafiltration test paper test, which uses colloidal gold and colloidal selenium as markers, can be quickly detected inside and outside the laboratory, and generally only needs 10-20 minutes Minutes, a single sample can be detected each time, but the detection sensitivity is low, it is not easy to be quantitative or semi-quantitative, and cannot meet the needs of many detections. Contents of the invention [000...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/543G01N33/545
Inventor 吴佩珊
Owner 吴佩珊
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