Application of tremella heteropolysaccharide or extract thereof to liquid dairy product
A technology of heteropolysaccharides and extracts, applied in the field of application of tremella heteropolysaccharides or its extracts in liquid dairy products, can solve the problems of increasing system complexity and instability, protein coagulation, fat floating, etc., to achieve stable milk Product system, protection of milk protein, effect of improving constipation
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Embodiment 1
[0061] The preparation method of embodiment 1 white fungus heteropolysaccharide extract
[0062] Soak the dried white fungus fruit body in water, after swelling, remove the yellow-brown base and clean it; put the washed raw material into an extraction tank, add water 20 times that of the dry material, stir and extract at 80°C for 1 hour, Then filter, and the supernatant finally passes through a filter with a pore size of 0.1-1 μm; under uniform stirring, fully mix the filtrate and ethanol, so that the final concentration of ethanol in the mixed liquid is 50%, collect the precipitate, dehydrate and decolorize with 95% ethanol , collect the precipitate; dry with hot air at 80°C, and pulverize. The product yield is 1.5% (in terms of dry white fungus weight).
Embodiment 2
[0063] The preparation method of embodiment 2 white fungus heteropolysaccharides
[0064] The tremella heteropolysaccharide extract of embodiment 1 is dissolved with 600 times of (weight) deionized water, after dissolving, add the chloroform of 200ml: the mixed solution of n-butanol=4: 1 (volume ratio), after fully mixing, centrifuge Machine centrifugation (4000r / min, 30 minutes), collect the supernatant. The centrifuged supernatant was mixed with the above-mentioned chloroform-butanol mixed solution at a ratio of 3:1 (volume ratio), and centrifuged with a centrifuge (4000 r / min, 30 minutes), and the supernatant was collected. Repeat the above operations until the OD value of the aqueous phase at 280 nm (using a 752 ultraviolet grating spectrophotometer, Shanghai Precision Scientific Instrument Factory) no longer decreases. The water phase was concentrated under reduced pressure to one-third, and the concentrated solution was fully mixed with 95% ethanol of 3 times the amount...
Embodiment 3
[0112] Embodiment 3 is to the stability of sour fruit juice milk
[0113] 1. Preparation of test samples:
[0114] (1) Containing the sample of tremella heteropolysaccharide or its extract: getting concentration is the sweet orange former fruit juice solution of the ethylparaben of 0.02wt%, adds the white granulated sugar of 5wt% (percentage is relative to the weight of sweet orange former fruit juice solution ), stirring and dissolving, adding solid white fungus heteropolysaccharide or white fungus heteropolysaccharide extract wherein, stirring rapidly makes all dissolving, is mixed with the fruit juice solution of the white fungus heteropolysaccharide or its extract that concentration is 0.05wt% respectively, adjusts solution pH=3.9 ,spare.
[0115] Get concentration and be 0.02wt% ethyl paraben aqueous solution, use it to prepare Tremella heteropolysaccharide or its extract solution of 0.05wt% respectively with it, add the white granulated sugar of 5wt% wherein (percentage...
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