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Method for processing disease control after strawberry is picked by combining ozone and Cryptococcus laurentii

A technology for Cryptococcus lorens and postharvest diseases, which is applied in the fields of food preservation, fruit and vegetable preservation, food science, etc., can solve problems such as affecting the use of antagonistic bacteria, achieve inhibition of fruit pathogens, significant economic and social benefits, and reduce effect of loss

Inactive Publication Date: 2012-03-28
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These factors have affected the use of antagonistic bacteria as fruit and vegetable fresh-keeping agents in production

Method used

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  • Method for processing disease control after strawberry is picked by combining ozone and Cryptococcus laurentii
  • Method for processing disease control after strawberry is picked by combining ozone and Cryptococcus laurentii
  • Method for processing disease control after strawberry is picked by combining ozone and Cryptococcus laurentii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: The control effect of ozone and Lorenti yeast in combination on strawberry fruit botrytis

[0025] 1. Test plan

[0026] The tested fruits have been carefully selected, and the fruit should be selected as uniform as possible, without scratches, mechanical injuries and pest damage on the surface. The fruit was punched with a sterile puncher (3 × 3 mm) and inserted into 5 × 10 4 Gray mold spores / ml, after 2 hours, carry out the following treatment: (1) Put the strawberries in a plastic basket and place them in a self-made plastic tent, turn on the ozone generator to ventilate for 30s (the ozone concentration is 0.574mg / ml at this time), Maintained for 10 min, the strawberries treated with ozone were treated with 1×10 8 individuals / ml of Cryptococcus laurentis suspension soaked for 30s, and air-dried naturally; (2) Add 30ul of Laurentia spp. 8 pcs / ml); (3) ozone aeration for 30s and maintaining for 10 min while measuring the ozone concentration in the ...

Embodiment 2

[0032] Embodiment 2: The influence of ozone and Laurent's yeast combined use on natural decay rate of strawberry fruit

[0033] 1. Test plan

[0034] The fruits were treated as follows: (1) Put the strawberries in a plastic basket and place them in a self-made plastic tent, turn on the ozone generator to ventilate for 30s (the ozone concentration at this time is 0.574mg / ml), and maintain it for 10 minutes. 1 x 10 for strawberries 8 individuals / ml of Cryptococcus laurentis suspension soaked for 30s and air-dried naturally; (2) use 1×10 8 Individuals / ml of Cryptococcus laurentis suspension soaked for 30s; (3) Ozone aeration for 30s (ozone concentration is 0.574mg / ml) and maintained for 10 minutes; (4) Without any treatment. Then, put the treated fruits into plastic baskets and seal them with plastic wrap, and store them in a constant temperature incubator at 20°C (RH 95%) for 3 days and in a freezer at 4°C for 7 days, then transfer them to an incubator at 20°C (RH 95%). Sto...

Embodiment 3

[0039] Example 3: Effects of Ozone and Cryptococcus laurentis on Strawberry Storage Quality

[0040] 1. Test plan

[0041] The fruits were treated as follows: (1) Put the strawberries in a plastic basket and place them in a self-made plastic tent, turn on the ozone generator to ventilate for 30s (the ozone concentration at this time is 0.574mg / ml), and maintain it for 10 minutes. 1 x 10 for strawberries 8 individuals / ml of Cryptococcus laurentis suspension soaked for 30s and air-dried naturally; (2) use 1×10 8 Individuals / ml of Cryptococcus laurentis suspension soaked for 30s; (3) Ozone aeration for 30s and maintained for 10 min; (4) Without any treatment. Then, put the treated fruits into plastic baskets and seal them with plastic wrap, and store them in a constant temperature incubator at 20°C (RH 95%) for 3 days and in a freezer at 4°C for 7 days, then transfer them to an incubator at 20°C (RH 95%). Store in a constant temperature incubator for 1 day, and take it out f...

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PUM

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Abstract

The invention discloses a method for processing disease control after the strawberry is picked by combining ozone and Cryptococcus laurentii, belonging to the biological control field after the fruits are picked. The method comprises the following steps: activating Cryptococcus laurentii, inoculating the Cryptococcus laurentii into NYDB culture medium for culturing 24h, centrifuging the Cryptococcus laurentii to obtain thalli, using sterile water to preparing the thalli into 1*108 / ml of thalli suspension; putting the strawberry into a plastic basket and arranging the strawberry into a self plastic curtain, starting an ozone generator for introducing air for 30s with the ozone concentration being 0.574mg / ml, maintaining for 10min, soaking the strawberry being processed by ozone in Cryptococcus lanrentii suspension for 30s, and naturally air drying the strawberry; and putting the strawberry into the plastic basket, sealing the plastic basket by a preservative film, and storing the strawberry under the room temperature. In the method, the ozone is utilized for improving the biological control effect of the Cryptococcus laurentii on the strawberry after being picked, the use is simple, the operation is convenient, the effect is good and the cost is low.

Description

technical field [0001] The invention relates to a method for preventing and controlling post-harvest strawberry diseases by combining ozone treatment and Cryptococcus lorensis treatment, and belongs to the field of post-harvest biological control of fruits. Background technique [0002] The loss caused by rot in the process of fruit picking, transportation and storage is huge, and it is also a problem of global concern. According to reports, in developed countries, 10%-30% of fresh products are rotted due to post-harvest diseases, while in developing countries that lack storage and transportation refrigeration equipment, the rate of post-harvest fruit rot is as high as 40%-50%. Foreign fruit storage and freshness accounts for more than 60% of the total fruit output. At present, the fruit storage capacity in my country is more than 8 million tons, which is less than 15% of the total. Among them, the storage capacity of cold storage and air-conditioning storage is 700,000 tons...

Claims

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Application Information

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IPC IPC(8): A23B7/155A23B7/152
Inventor 张红印姜松任晓锋马龙传赵利娜
Owner JIANGSU UNIV
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