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Composite bacteria for treating food waste percolate and preparation method thereof

A technology of food waste and leachate, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of microbial nutrient element ratio imbalance and ratio imbalance, and achieve good results

Active Publication Date: 2012-03-28
SHANDONG SUKAHAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

4. The proportion of microbial nutrient elements in the leachate is out of balance, mainly due to the imbalance of C, N and P
Anaerobic treatment includes upward flow sludge bed, anaerobic immobilized bioreactor, mixed reactor and anaerobic stabilization pond. However, due to the extremely high content of COD, metal ions, ammonia nitrogen, etc. in landfill leachate, no industry has been formed so far. Simplified microbial products can play a role in biodegradation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] High-density cultivation of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Saccharomyces cerevisiae, Bacillus licheniformis, Trichobacterium nitrosatus and Nitrobacter victoria:

[0051] (1) Trichoderma viride: Inoculate the Trichoderma viride strain on the purified slant medium into a shake flask filled with an activated medium with a volume ratio of 15%, and the activated medium is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 180rpm, activate and cultivate for 32 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 50% fermentation medium, and the inoculum volume is 1% by volume , the formula of the fermentation medium is glucose 8g / l, corncob 5g / l, calcium chloride 0.2g / l, magnesium sulfate 0.1g / l, potassium dihydrogen phosphate 18g / l, yeast extract 8g / l, sulfuric acid Ferrous 3mg / l, zinc sulfate 1.0mg / l, coba...

Embodiment 2

[0061] High-density cultivation of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Saccharomyces cerevisiae, Bacillus licheniformis, Trichobacterium nitrosatus and Nitrobacter victoria:

[0062] (1) Trichoderma viride: Inoculate the Trichoderma viride strain on the purified slant culture medium into a shaker flask filled with a 30% volume ratio of activated medium, which is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 140rpm, activate and cultivate for 48 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 70% fermentation medium, and the inoculum volume is 3% by volume , the formula of the fermentation medium is glucose 12g / l, corn cob 6g / l, calcium chloride 0.6g / l, magnesium sulfate 0.5g / l, potassium dihydrogen phosphate 22g / l, yeast extract 12g / l, sulfuric acid Ferrous 7mg / l, zinc sulfate 1.8mg / l, cobalt chloride 4.0...

Embodiment 3

[0072]High-density cultivation of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Saccharomyces cerevisiae, Bacillus licheniformis, Trichobacterium nitrosatus and Nitrobacter victoria:

[0073] (1) Trichoderma viride: inoculate the Trichoderma viride strain on the purified slant medium into a shaker flask filled with an activated medium with a volume ratio of 20%, and the activated medium is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 160rpm, activate and cultivate for 36 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 60% fermentation medium, and the inoculum volume is 2% by volume , the formula of the fermentation medium is glucose 10g / l, corn cob 5.5g / l, calcium chloride 0.4g / l, magnesium sulfate 0.3g / l, potassium dihydrogen phosphate 20g / l, yeast extract 10g / l, Ferrous sulfate 5mg / l, zinc sulfate 1.5mg / l, cobal...

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Abstract

The invention discloses composite bacteria for treating food waste percolate and a preparation method thereof, and the preparation method comprises the following steps: respectively performing high-density culture on strains of trichoderma viride, bacillus subtilis, bread mold, photosynthetic bacteria, saccharomyces cerevisiae, bacillus licheniformis, nitrosomonas europaea and nitrobacterwinogradskyi, performing dehydration and drying on various types of monoxenie obtained by high-density culture to prepare hypopus microbial dry powder, and then mixing according to the weight ratio of 5-15 parts of the trichoderma viride, 10-15 parts of the bacillus subtilis, 20-30 parts of the bread mold, 10-15 parts of the photosynthetic bacteria, 7-15 parts of the saccharomyces cerevisiae, 10-15 parts of the bacillus licheniformis, 10-20 parts of the nitrosomonas europaea and the 10-20 parts of the nitrobacterwinogradskyi to prepare the composite bacteria for treating the food waste percolate. By using the composite bacteria for treating the waste percolate, the use is convenient, the effects of fermentation and degradation are good and the production cost is low.

Description

technical field [0001] The invention relates to the technical field of bioengineering microbial fermentation, in particular to a composite bacterium for treating kitchen waste leachate and a preparation method thereof. Background technique [0002] The moisture content of food waste can reach more than 90wt%, so it is easy to produce a large amount of leachate during the stacking process. The leachate of food waste has the following characteristics: 1. The water quality is complex and harmful. Studies have shown that by using GC-MS technology to analyze the components of organic pollutants in landfill leachate, a total of 63 kinds of organic pollutants were detected in landfill leachate, and 34 kinds had a reliability of more than 60%. Among them, there are 6 kinds of alkenes, 19 kinds of carboxylic acids, 5 kinds of esters, 10 kinds of alcohols and phenols, 10 kinds of aldehydes and ketones, 7 kinds of amides, 1 kind of aromatic hydrocarbons and 5 kinds of others. Among th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/14C02F3/34C12R1/885C12R1/125C12R1/845C12R1/865C12R1/10
Inventor 韩威华于伟陈永科
Owner SHANDONG SUKAHAN BIO TECH
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