Digestive juice for separating mouse fat stem cells

A technology of fat stem cells and digestive juice, which is applied in the field of separation of animal stem cells, can solve the problems of not being able to maintain the nutritional needs of cells well, and achieve the effect of low cost, good activity, and maintenance of activity

Inactive Publication Date: 2012-03-28
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] By comprehensively considering the problems existing in the DPBS digestion solution containing 1% BSA in the existing solution and the necessity of adding glucose and adenosine acid into the digestion solution, we believe that the main problem of the former lies in the digestion process of nearly one hour, only Adding BSA cannot well maintain the nutritional requirements of cells during this process and maintain their activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] In this embodiment, the following three experimental groups were designed according to the different proportions of the components. Each group took the same amount of adipose tissue and digested them with the following three different proportions of digestive juice.

[0015] The specific components of the three digestive juices are as follows (10ml system);

[0016] (1) Digestive juice one

[0017] Element

Proportion

Amount added

fetal bovine serum

0

0

Collagenase I

0.1％

0.01g

DMEM / F12

10ml

[0018] (2) Digestive juice two

[0019] Element

Proportion

Amount added

fetal bovine serum

10％

1ml

Collagenase I

0.09％

0.009g

DMEM / F12

9ml

[0020] (3) Digestive juice three

[0021] Element

Proportion

Amount added

fetal bovine serum

20％

2ml

Collagenase I

0.08％

0.008g...

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Abstract

The invention discloses digestive juice for separating mouse fat stem cells. The digestive juice comprises 10% of fetal calf serum, 0.09% of collagenase I and the balance of DMEM/F12 (dulbecco's modified eagle medium/nutrient mixture F-12). The digestive juice is simple in components, convenient in source and low in cost, and can effectively separate the fat stem cells from fat tissues of a mouse, well maintain the activity of the cells and lay a foundation for late-stage culture of the cells and further scientific research.

Description

[technical field] [0001] The invention relates to the technical field of separation of animal stem cells, in particular to a digestive fluid for separating mouse fat stem cells. [Background technique] [0002] Adipose stem cells have the potential of multidirectional differentiation, and can be directional differentiated into fat, osteoblast, cartilage, liver, cardiomyocytes, etc. in vitro. The advantages of time donor pain, so it has important significance in regenerative medicine. [0003] As an important model animal, mice are widely used in basic research, but the in vitro culture of mouse adipose stem cells has always been a difficult problem, which is reflected in all aspects from isolation to culture. The isolation of mouse adipose stem cells generally uses enzymes Digestion, as the initial stage of isolation and culture, often leads to many problems in the later culture of cells if a suitable digestion system is not used. [0004] At present, there are also some re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 魏超张运海刘星李运生章孝荣陈建文周娜汝桂涛张宇陶佳吴蓉花
Owner ANHUI AGRICULTURAL UNIVERSITY
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