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Preparation method of 2-phenethyl alcohol

A technology for phenethyl alcohol and ethanol, applied in the field of biological preparation of 2-phenethyl alcohol, can solve problems such as poisoning, low yield of 2-phenethyl alcohol, inhibition of biotransformation of 2-phenethyl alcohol, etc. the possibility of reducing the effect of the process of growth and reproduction

Inactive Publication Date: 2012-03-28
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the precursor of biotransformation of 2-phenylethanol is L-phenylalanine produced by fermentation method or enzymatic method. The carbon source generally uses common sugars such as glucose, sucrose, and molasses. Due to the metabolism of glucose, sucrose, etc., a large amount of ethanol is produced , thereby significantly inhibiting and poisoning the biotransformation of 2-phenylethanol. Studies have shown that in addition to the inhibition of ethanol that exists alone on the biosynthesis of 2-phenylethanol, when ethanol and 2-phenylethanol exist in the biotransformation system at the same time, The synergistic inhibition of the product 2-phenylethanol was more pronounced, resulting in a lower yield of 2-phenylethanol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The high-activity dry yeast used in this example is the commercially available Angel Yeast High-Activity Dry Yeast, Angel Yeast Co., Ltd., with a trade name of "Angel High-Activity Dry Yeast".

[0029] A preparation method of 2-phenylethyl alcohol, comprising the following steps:

[0030] (1) Put 0.25g of highly active dry yeast into a 250mL shake flask containing 50mL of fermentation medium, control the temperature of the fermentation process to 25°C, the rotation speed to 100rpm / min, and the time to 15h, and the fermentation ends;

[0031] The amount of active dry yeast powder inserted into the fermentation medium is calculated according to its weight-to-volume ratio to the fermentation medium, that is, active dry yeast: fermentation medium is 0.5% (W / V);

[0032] The fermentation medium is calculated per liter, and its composition is, L-phenylalanine 5g, ethanol 2g, K 2 HPO 4· 3H 2 O 0.3g, MgSO 4· 7H 2 O 0.1g, NaCl 0.5g, the balance is water, pH is natural, ster...

Embodiment 2

[0038] The high-activity dry yeast used in this example is Danbaoli high-activity dry yeast, Guangzhou Danbaoli Yeast Co., Ltd., trade name "Danbaoli high-activity dry yeast".

[0039] A preparation method of 2-phenylethyl alcohol, comprising the following steps:

[0040] (1) Insert 1.5g of highly active dry yeast into a 250mL shake flask with 50mL of fermentation medium, control the temperature of the fermentation process to 38°C, the rotation speed to 300rpm / min, and the time to 60h, and the fermentation ends;

[0041] The amount of active dry yeast powder inserted into the fermentation medium is calculated according to its weight-to-volume ratio to the fermentation medium, that is, active dry yeast: fermentation medium is 3% (W / V);

[0042] The fermentation medium is calculated per liter, and its composition is, L-phenylalanine 30g, ethanol 30g, K 2 HPO 4· 3H 2 O 1.5g, MgSO 4· 7H 2 O 1.5g, NaCl 1g, the balance is water, pH is natural, sterilized by high pressure steam ...

Embodiment 3

[0048] The high-activity dry yeast used in this example is Panyu Meishan-Mali Instant High-Activity Dry Yeast, Panyu Meishan-Mali Yeast Co., Ltd., and the trade name is "Meishan Instant Dry Yeast".

[0049] A preparation method of 2-phenylethyl alcohol, comprising the following steps:

[0050] (1) Insert 0.6g of highly active dry yeast into a 250mL shake flask with 50mL of fermentation medium, control the temperature of the fermentation process to 36°C, the rotation speed to 200rpm / min, and the time to 48h, and the fermentation ends;

[0051] The amount of the active dry yeast powder inserted into the fermentation medium is calculated according to its weight-to-volume ratio to the fermentation medium, that is, active dry yeast: fermentation medium is 1.2% (W / V);

[0052] The fermentation medium is calculated per liter, and its composition is, L-phenylalanine 10g, ethanol 20g, K 2 HPO 4· 3H 2 O 1g, MgSO 4· 7H 2 O 1.5g, NaCl 0.75g, the balance is water, pH is natural, steri...

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Abstract

The invention discloses a preparation method of 2-phenethyl alcohol, comprising the following step of preparing 2-phenethyl alcohol in a fermenting way by the means that active dry yeast which can be used for oxidatively degrading alcohol is selected, L-phenylalanine is taken as a substrate, and the alcohol is taken as a sole carbon source. In the fermenting process, the temperature is controlled to be within the range of 25-38 DEG C, the rotation speed is controlled to be within the range of 100-800 rpm / min, the time is controlled to be within the range of 15-60 hours, the initial concentration of alcohol is controlled to be within the range of 2-30g / L, and the concentration of 2-phenethyl alcohol is controlled to be within the range of 0.68-3.81g / L after the fermentation is ended. The alcohol is oxidatively degraded by the active dry yeast and is further transformed into 2-phenethyl alcohol, the restraint of the high-concentration alcohol in fermentation liquor to the synthesis of the product 2-phenethyl alcohol is overcome, the probability of microbiological contamination in the process of biotransformation is reduced, the content of organic impurities in 2-phenethyl alcohol fermentation liquor is reduced, the use ratio of the carbon source is observably improved, and the preparation method has the characteristics of being convenient to operate, low in production cost, wide in industrialized application prospect, etc.

Description

technical field [0001] The invention relates to a biological preparation method of 2-phenylethanol. Background technique [0002] 2-phenylethanol (2-phenylethanol, 2-PE), also known as β-phenylethanol and diphenylethanol, is a kind of aromatic alcohol with rose fragrance. In plant essential oils such as jasmine, 2-phenylethanol is also a flavor substance in bread, cheese, biscuits, wine and other foods. This substance is stable in alkaline environment and oxidation to air, and its derivative esters, such as phenylethyl acetate It is also an important aromatic product. Because of its soft and sweet aroma, 2-phenylethanol is not only the basic component of all rose fragrances, but also has a synergistic effect, and is a required ingredient for various fragrance formulations. At present, 2-phenylethanol is the second largest fragrance product, its consumption is second only to vanillin, and it is widely used in food, tobacco, cosmetics and daily cleaning products. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/22
Inventor 荣绍丰丁保妹管世敏陈奕涵张晓丽
Owner SHANGHAI INST OF TECH
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