Fox growth hormone releasing hormone cDNA and its application

A growth hormone and hormone-releasing technology, which is applied in the field of fox growth hormone-releasing hormone cDNA, can solve problems affecting cell activity, etc., and achieve the effects of obvious weight gain, growth promotion, and faster growth speed

Inactive Publication Date: 2012-04-04
CHINA AGRI UNIV
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  • Abstract
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Problems solved by technology

The increase of cAMP level will promote the conformational change and activation of the regulatory subunit of cAMP-d

Method used

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  • Fox growth hormone releasing hormone cDNA and its application
  • Fox growth hormone releasing hormone cDNA and its application
  • Fox growth hormone releasing hormone cDNA and its application

Examples

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[0026] The specific embodiments of the present invention will be described in further detail below in conjunction with the drawings and embodiments. The following examples are used to illustrate the present invention, but not to limit the scope of the present invention.

[0027] Take the nucleotide sequence of the mature fox GHRH peptide as the target gene, and pVAX1 as the eukaryotic expression vector, insert the target gene into the vector to construct a eukaryotic expression vector, and then intramuscularly inject it into the fox’s leg muscles to obtain the growth rate of the fox Speed ​​up, weight gain is obvious result.

[0028] The vector construction process is as follows: use PCR method to connect the signal peptide gene sequence of human growth hormone and the mature peptide gene sequence of fox growth hormone releasing hormone in series, connect to the T vector (pMD19-GHRHS), and then use BamH I and EcoR I to double Cut the target gene on the T vector and the empty pVAX1...

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Abstract

The invention discloses a full-length nucleotide sequence and a mature peptide nucleotide sequence of a fox growth hormone releasing hormone cDNA, and amino acid sequences of proteins coded respectively by the full-length nucleotide sequence and the mature peptide nucleotide sequence. The fox growth hormone releasing hormone cDNA is a complete open reading frame, comprises 321 nucleotide sequences, and can code 106 amino acids. The mature peptide nucleotide sequence of the fox growth hormone releasing hormone cDNA comprises is coded by 132 nucleotide sequences and 44 amino acid residues are coded. The fox growth hormone releasing hormone cDNA can be utilized for growth promotion of economic animals such as fox and the like.

Description

technical field [0001] The invention relates to cDNA of fox growth hormone releasing hormone obtained by cloning and application thereof. Background technique [0002] The fox is a fur animal with high economic value. Its growth is mainly controlled by the growth hormone produced and released by the pituitary gland. After reaching body maturity, the production and release of growth hormone decreases. Growth hormone is under the dual control of growth hormone releasing hormone and growth hormone releasing inhibitory hormone produced by the hypothalamus of the animal itself. Growth hormone-releasing hormone stimulates the production and release of growth hormone by the pituitary gland, while growth hormone-releasing inhibitory hormone inhibits the production of growth hormone. The two work together to maintain the growth rate of the fox. Growth hormone-releasing hormone is produced in the arcuate nucleus and ventromedial nucleus of the hypothalamus. After synthesis, it is tr...

Claims

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Application Information

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IPC IPC(8): C12N15/63C07K14/60C12N1/19C12N1/21C12N5/10A61K38/25C12N15/16C12N1/15A61P43/00A61K48/00
Inventor 王吉贵刘维全刘芃芃齐顺章
Owner CHINA AGRI UNIV
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