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Plant stress tolerance related protein TaSnRK2.10 as well as coding gene and application thereof

A technology for encoding genes and proteins, applied in the field of plant stress tolerance-related protein TaSnRK2.10 and its encoding genes and applications

Active Publication Date: 2012-04-04
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The research on the SnRK2 family started relatively late, but more and more evidences show that the expression of most members of the family is induced by osmotic stress, and some members are also involved in the transmission process of ABA signal

Method used

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  • Plant stress tolerance related protein TaSnRK2.10 as well as coding gene and application thereof
  • Plant stress tolerance related protein TaSnRK2.10 as well as coding gene and application thereof
  • Plant stress tolerance related protein TaSnRK2.10 as well as coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1. Isolation of the gene TaSnRK2.10 encoding the stress-resistance-related protein in wheat

[0048] 1. Isolation of TaSnRK2.10, the gene encoding anti-stress-related protein

[0049] 1. Construct the full-length cDNA library of wheat, according to the literature (Mao Xinguo et al., 2005, use the improved Cap-trapper method to construct the full-length cDNA library of I. spelter. Acta Genetics, 32 (8): 811-817) The method described in:

[0050] (1) Total RNA extraction and mRNA purification, wheat total RNA was extracted with TRIZOL, and mRNA was separated and purified with oligo(dT) cellulose.

[0051] (2) Synthesis of first-strand cDNA: Mix 10ug mRNA with primer I, add reagents for first-strand cDNA synthesis after denaturation, when the temperature rises to 40°C, add reverse transcriptase, and when the reaction reaches 40 minutes Primer II was added (first strand synthesis primers below). In order to obtain more full-length cDNA, trehalose and behenitol we...

Embodiment 2

[0075]Example 2, gene TaSnRK2.10 and its application in Arabidopsis

[0076] 1. Acquisition of TaSnRK2.10

[0077] Design primers: upstream primer F1: 5'-CCCAAGCTTGTGGGGGAAGGAAAGGGG-3' (Hind III site, sequence 3), downstream primer RI: 5'-ACGC GTCGACCATAGCATACACTATCTCCCCGCT-3' (Sal I site, sequence 4), wherein downstream primer 3 ' end is located upstream of the gene stop codon.

[0078] The mRNA of common wheat (Triticum aestivum L. Hanxuan No. 10, preserved by the National Crop Germplasm Bank) was extracted and obtained by RT-PCR using 5′-GTGGGGGAAGGAAAGGGG-3′ and 5′-AGTAACATTTGTGGGCGGGC-3′ as primers The full-length cDNA of gene TaSnRK2.10 in wheat;

[0079] Using the full-length cDNA sequence of the gene TaSnRK2.10 as a template, using the above-mentioned primers F1 and R1, the high-fidelity enzyme Pfu was used to amplify the target gene, and a PCR product of 1119bp was obtained, which was sent for sequencing. The result was that the PCR product had the sequence in the s...

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Abstract

The invention discloses a plant stress tolerance related protein TaSnRK2.10 as well as a coding gene and an application thereof. According to the invention, the protein is the protein shown by the following (a) or (b): (a) a protein formed by amino acid sequences shown in a sequence 2 in a sequence table; and (b) a protein obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequences in the sequence 2 in the sequence table, related to plant stress tolerance and derived from the (a). According to the invention, it is proved by experiments that the plant stress tolerance, like drought tolerance, water conservation, salt resistance or cold resistance, can be improved by introducing the genes into the plant. Therefore, according to the invention, the genes and the application thereof has important realistic meaning to develop new drought tolerant, water saving, salt resistant or cold resistant plant.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a plant stress tolerance-related protein TaSnRK2.10 and its coding gene and application. Background technique [0002] Drought and water shortage is a serious problem faced by global agricultural production, and it is also an important factor restricting the development of my country's grain production. The cultivation of wheat, the main food crop, requires a lot of water. On average, 1 ton of wheat needs 500-700m of water in my country. 3 . At least 60 million hectares of wheat are cultivated on rainfed land in developing countries all over the world, but the yield level is only 10%-50% of that under irrigated conditions. Therefore, the development of drought-tolerant and water-saving wheat varieties to improve the water use efficiency of crops can not only increase the yield, but also alleviate the contradiction of water shortage. [0003] Improving the drought tolerance of crop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/12C12N15/54C12N15/63C12N1/21C12N5/10C12N15/09C12N15/84A01H5/00
Inventor 毛新国张洪映景蕊莲
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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