Method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution

A technology of blanching liquid and glycoprotein, applied in the direction of protein food ingredients, fish protein ingredients, applications, etc., to achieve the effect of improving immunity, increasing the number of white blood cells, and assisting anti-tumor biological activity

Active Publication Date: 2012-04-11
SHANDONG HOMEY AQUATIC DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there has been no report on extracting sea cucumber glycoprotein from sea cucumber blanching liquid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution, the specific process steps are as follows:

[0026] (1) Weigh 2000L of sea cucumber blanching liquid as raw material, and concentrate it to a volume of 150L with a vacuum concentration equipment, with a solid content of about 40%;

[0027] (2) Add ethanol with a concentration of 95% to the obtained concentrated solution for precipitation, so that the final concentration is between 60%;

[0028] (3) Centrifuge the ethanol-precipitated solution with a three-legged centrifuge at a controlled speed of 2000rpm to collect the precipitate;

[0029] (4) After drying the collected precipitate, use a pulverizer to make powder;

[0030] (5) Add the powder into an ethanol solution with a concentration of 70%, stir for desalination treatment, collect the precipitate by pressure filtration after desalination, and repeat the desalination treatment for 3 times according to the above scheme;

[...

Embodiment 2

[0036] A method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution, the specific process steps are as follows:

[0037] (1) Weigh 6000L of sea cucumber blanching liquid as raw material, and concentrate it to a volume of 400L with a vacuum concentration equipment, with a solid content of about 37%;

[0038] (2) Add ethanol with a concentration of 95% to the obtained concentrated solution for precipitation, so that the final concentration is between 65%;

[0039] (3) Centrifuge the ethanol-precipitated solution with a three-legged centrifuge at a controlled speed of 2000rpm to collect the precipitate;

[0040] (4) After drying the collected precipitate, use a pulverizer to make powder;

[0041] (5) Add the powder into an ethanol solution with a concentration of 65%, stir for desalination treatment, collect the precipitate by pressure filtration after desalination, and repeat the desalination treatment for the precipitate twice according to the abov...

Embodiment 3

[0047] A method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution, the specific process steps are as follows:

[0048] (1) Weigh 2000L of sea cucumber blanching liquid as raw material, and concentrate it to a volume of 200L with a vacuum concentration equipment, with a solid content of about 20%;

[0049] (2) Add ethanol with a concentration of 95% to the obtained concentrated solution for precipitation, so that the final concentration is between 85%;

[0050] (3) Centrifuge the ethanol-precipitated solution with a three-legged centrifuge at a controlled speed of 2000rpm to collect the precipitate;

[0051] (4) After drying the collected precipitate, use a pulverizer to make powder;

[0052] (5) Add the powder into an ethanol solution with a concentration of 85%, stir for desalination treatment, collect the precipitate by pressure filtration after desalination, and repeat the desalination treatment for the precipitate twice according to the abov...

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PUM

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Abstract

The present invention relates to a method for preparing sea cucumber glycoprotein by using a sea cucumber blanching solution. The method comprises that: a sea cucumber blanching solution is adopted as a raw material, and is subjected to treatments of reduced pressure condense, alcohol precipitation, drying to prepare into powder; the powder is subjected to treatments of alcohol leaching and desalination, and then is redissolved in purified water; the resulting solution is subjected to treatments of enzymolysis and centrifugation; the resulting supernatant is subjected to treatments of tube ultrafiltration, ultrafiltration and drying to obtain the sea cucumber glycoprotein product. The preparation method of the present invention has characteristics of low equipment investment, simple operation, short production period, high yield and low product cost. The prepared sea cucumber glycoprotein has good biological activity.

Description

technical field [0001] The invention relates to a treatment of sea cucumber working liquid, in particular to a method for preparing sea cucumber glycoprotein by using sea cucumber blanching liquid. Background technique [0002] It has been verified by research that sea cucumbers (mainly sea cucumbers) contain more than 50 kinds of nutrients that are beneficial to human physiological activities, among which the protein content is relatively high, and its protein contains 18 kinds of amino acids, rich in saponins, mucopolysaccharides, gangliosides, Various active substances such as sterols and collagen; rich in trace elements, including calcium, phosphorus, iron, magnesium, zinc, selenium, alum, manganese, etc.; and vitamins such as vitamins B1, B2 and niacin. It can continue the aging of the human body, invigorate the kidneys and Qi, strengthen the essence and marrow, and eliminate fatigue. Because sea cucumber has many physiological and pharmacological activities, it is inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04
Inventor 袁文鹏胡炜孙永军王志伟刘昌衡张绵松孟秀梅唐聚德
Owner SHANDONG HOMEY AQUATIC DEV
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