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Preparation method for compound stevioside of RA (rebaudioside A) and RD (rebaudioside D)

A technology of stevioside and stevia, applied in the extraction and preparation of medicinal materials, and the preparation of RA and RD compound stevioside, can solve the problems of no preparation method for compound products, and achieve low cost, no aftertaste, and good taste

Active Publication Date: 2012-04-11
NINGBO GREEN HEALTH PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no RD and RA compound stevioside product on the market, and there is no report on the preparation method of the compound product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of preparation method of RA and RD compound stevioside provided by the present embodiment, its steps are as follows:

[0029] (1) Water extraction step: put 1 ton of crushed stevia into the extraction equipment continuously at a rate of 200kg / h-260kg / h, add water 5 times the amount of raw materials for extraction, and extract at 60°C for 2 hours filter;

[0030] (2) Resin separation step: the filtrate is passed through the HPD-600 macroporous resin, eluting with 70% ethanol solvent of 3L, taking 500L as the unit segmental interception eluate, using liquid chromatography to analyze and detect RA, RD Content, the content parameters of RA and RD in the eluate stevioside after 500L are compared in the following table:

[0031] Chromatography object

RA

RD

500L

32.72％

27.96％

1000L

35.45％

20.44％

1500L

39.47％

15.36％

2000L

46.81％

10.17％

2500L

58.36％

3...

Embodiment 2

[0038] A kind of preparation method of RA and RD compound stevioside, its steps are as follows:

[0039] (1) Extraction step: put 1 ton of crushed stevia into the extraction equipment continuously at a rate of 200kg / h-260kg / h, add water 10 times the amount of the raw material for extraction, control the temperature at 80°C, and filter after the extraction is completed ;

[0040] (2) Resin separation step: pass the filtrate through HPD-600 macroporous resin, elute with 3L of 80% ethanol solvent, collect the eluate in sections, intercept 2000L, pass the eluate through an anion exchange resin, and take the outflow liquid part;

[0041] (3) Decolorization step: Take the effluent part and add activated carbon of 1 / 30 times the amount of the raw material, decolorize at a temperature of 80°C for 30 minutes, filter, and dry to obtain the crude stevioside. The RA content in the stevioside crude product is 52.85%, and the RD content 11.48%;

[0042] (4) Crystallization step: at a tem...

Embodiment 3

[0046] A kind of preparation method of RA and RD compound stevioside, its steps are as follows:

[0047] (1) Extraction step: put 1 ton of crushed stevia into the extraction equipment continuously at a rate of 200kg / h-260kg / h, add water 10 times the amount of the raw material for extraction, control the temperature at 90°C, and filter after the extraction is completed ;

[0048] (2) Resin separation step: pass the filtrate through HPD-600 macroporous resin, elute with 3L of 90% methanol, desorb and collect the eluate in sections, intercept 1500L, pass the eluate through an anion exchange resin, and take the outflow liquid part;

[0049] (3) Decolorization step: take the effluent part and add activated carbon of 1 / 40 times the amount of the raw material, decolorize at a temperature of 85 ° C for 40 minutes, filter, and dry to obtain the crude stevioside. The RA content in the stevioside crude product is 43.72%, and the RD content 16.31%;

[0050] (4) Crystallization step: at a...

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Abstract

The invention discloses a preparation method for compound stevioside of RA and RD. The method is characterized by comprising the following steps in order: (1) extracting crushed stevia rebaudiana leaves with water and conducting filtration; (2) passing the filtrate through a macroporous resin column, carrying out eluting with an alcoholic solvent, passing the eluent through ion exchange resin, and taking the effluent liquid; (3) adding a decoloring agent into the effluent liquid, conducting filtering and drying, thus obtaining a stevioside crude product; (4) adding an alcoholic solvent for dissolving, performing reflux heating, and during the process, adding the same amount of water as the stevioside crude product so as to make the stevioside crude product dissolved in the system completely; then implementing standing and filtering so as to obtain a crystal; (5) carrying out dissolution with an alcoholic solvent as well as reflux heating again, and adding water for repeated crystallization; (6) conducting washing with an alcoholic solvent and drying, thus obtaining a finished product. The invention has the advantages of: low cost, easy crystallization component control; good mouthfeel of the prepared compound stevioside of RA and RD, no remaining taste, adaptation to market needs; and better product stability.

Description

technical field [0001] The invention belongs to the field of medicine preparation, and relates to a method for extracting and preparing medicinal materials, in particular to a method for preparing RA and RD compound stevioside. Background technique [0002] Stevioside is a high-sweetness, low-calorie, new-type, health-care, natural sweetener. The product has no residue in the human body, is safe and non-toxic, is beneficial to regulate blood sugar, promotes mental activity, has auxiliary therapeutic functions for patients with cardiovascular and cerebrovascular diseases, obesity, and diabetes, and is more helpful in preventing dental caries and as a phenylketouretic ketone ( Carbohydrate substitutes for PKU) patients. [0003] Steviosides mainly include stevioside, stevioside A, rubusoside, dulcoside A (dulcosideA), stevioside C, stevioside F, stevioside D, stevioside, stevioside nine Stevioside A is the main component, and most of the stevioside products currently on the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/09C07H15/256C07H1/08
Inventor 戴珊媛卢国定
Owner NINGBO GREEN HEALTH PHARMACEUTICAL CO LTD
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