Method for extracting and separating rosmarinic acid and ursolic acid from rosemary

A technology of rosmarinic acid and rosemary, which is applied in the separation/purification of carboxylate, steroids, organic chemistry, etc., can solve the problems of low yield, low cost, large content of active ingredients, etc. The effect of reducing production cost, simple operation and improving economic efficiency

Inactive Publication Date: 2012-05-02
长沙卫一生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0023] The purpose of the present invention is to provide a method for extracting and separating rosmarinic acid and ursolic acid from rosemary. Content and yield are not high, and a method of using rosemary a

Method used

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  • Method for extracting and separating rosmarinic acid and ursolic acid from rosemary
  • Method for extracting and separating rosmarinic acid and ursolic acid from rosemary
  • Method for extracting and separating rosmarinic acid and ursolic acid from rosemary

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Weigh 100Kg of rosemary, pulverize it, place it in 300L (V / V) in 60% ethanol for 4 hours under vacuum at 40°C, filter it once with a ceramic membrane; add the filter residue to 200L of 60% ethanol, keep it under vacuum at 40°C for 4 hours, and then filter the The membrane was filtered once more, and the two filtrates were combined; the filtrate was added with an appropriate amount of antioxidant 150g and concentrated in vacuo at 50°C until 12kg of dry matter was obtained; 60L of 30% ethanol was used to dissolve the obtained dry matter, and the ceramic membrane was filtered once, and the filtrate was filtered. LSA-10 macroporous resin column, after adsorption, remove impurities with 60L petroleum ether and 60L ethyl acetate respectively, desorb with 60L pH3.5, 45% ethanol solution, and collect the eluate A; 16L of acetone was dissolved, filtered, and the filtrate was placed on an LSA-10 macroporous resin column. After adsorption, it was decontaminated with petroleum ether...

Embodiment 2

[0050] Weigh 200Kg of rosemary, pulverize it, place it in 600L (V / V) in 60% ethanol for 4 hours under vacuum at 40°C, filter it once with a ceramic membrane; add the filter residue to 400L of 60% ethanol, keep it under vacuum at 40°C for 4h, and then filter the The membrane was filtered once again, and the filtrates were combined twice; the filtrate was added with an appropriate amount of antioxidant 250g and concentrated in a vacuum at 50 ° C until 25kg of dry matter was obtained; the obtained dry matter was dissolved with 125L of 30% ethanol, and activated carbon was added to decolorize the ceramic for 15min. The membrane was filtered once, and the filtrate was applied to LSA-10 macroporous resin column. After adsorption, 120L of petroleum ether and 120L of ethyl acetate were used to remove impurities, and 120L of pH3.5, 45% ethanol solution was used for desorption, and the elution was collected. Material A; 20 kg of filter residues were dissolved with 40 L of acetone, filter...

Embodiment 3

[0052]Weigh 500Kg of rosemary, pulverize it, place it in 1500L (V / V) in 60% ethanol for 4 hours under vacuum at 40°C, filter and filter it once with a ceramic membrane; add the filter residue to 1000L of 60% ethanol, keep it under vacuum at 40°C for 4h, The ceramic membrane was filtered once again, and the two filtrates were combined; the filtrate was added with an appropriate amount of antioxidant 500g and concentrated in vacuo at 50°C until 70kg of dry matter was obtained; the obtained dry matter was dissolved with 350L of 30% ethanol, and activated carbon was added for decolorization for 15min. The ceramic membrane was filtered once, and the filtrate was applied to LSA-10 macroporous resin column. After adsorption, 300L of petroleum ether and 300L of ethyl acetate were used to remove impurities, and 300L of pH3.5, 45% ethanol solution was used for desorption. Removal A; 45kg of filter residue was dissolved with 90L of acetone, filtered, and the filtrate was placed on LSA-10 ...

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Abstract

The invention mainly relates to a method for extracting and separating rosmarinic acid and ursolic acid from rosemary. The method comprises the following steps of: grinding the rosemary serving as a raw material, extracting, concentrating, performing column chromatographic separation, extracting, concentrating, crystallizing, recrystallizing, and thus obtaining the rosmarinic acid and the ursolicacid. The whole separation process has low requirements on environmental conditions and equipment, the rosmarinic acid and the ursolic acid have high yield, the shortest separation time, a simple operation method and high purity, and the raw material has the highest comprehensive utilization rate; in addition, the separation materials are readily available and cheap, and the separation operation process is simple and is easy to control; and crystallization and recrystallization are utilized, so that the method is high in purification efficiency and low in cost.

Description

Technical field: [0001] The invention belongs to the field of natural product chemistry, and specifically relates to a method for extracting and separating antioxidants from rosemary, the main active components of which are rosmarinic acid and ursolic acid. Background technique [0002] Antioxidants are used to prevent or delay the oxidation of animal and vegetable oils, oily foods, instant noodles, meat products and beverages, prolong the storage period of products, or achieve the purpose of preservation. Antioxidants are mainly divided into natural antioxidants. Researchers have conducted high-dose animal experiments on synthetic antioxidants and natural antioxidants. The results show that synthetic antioxidants not only significantly cause hepatomegaly, but also increase the activity of liver and lung microsomal enzymes. Convert other substances ingested by the human body into toxic substances or carcinogens The United States has eliminated the use of synthetic antioxidan...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48C07J63/00
Inventor 刘喜荣刘正芳王腊霜
Owner 长沙卫一生物科技有限公司
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