High flux detection method and biochip based on nucleic acid address coding
A technology of address coding and detection methods, which is applied in the field of high-throughput detection methods and biochips based on nucleic acid address coding, can solve the problems of limiting the progress of proteomics research and the lack of biochips for protein detection methods, and achieve improved detection types, Reliable test results and good stability
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[0029] A high-throughput detection method based on nucleic acid address coding, comprising the following steps:
[0030] 1) immobilizing the first single-stranded nucleic acid arrangement on a solid phase carrier;
[0031] 2) coupling a detection substance capable of specifically reacting with the analyte to a second single-stranded nucleic acid to obtain a detection body, wherein the second single-stranded nucleic acid is complementary to the first single-stranded nucleic acid;
[0032] 3) Mix and react the analyte with the analyte, then add the labeled secondary antibody, and continue the reaction to obtain the analyte-analyte-secondary antibody complex; combine the above complex with the first single-chain immobilized on the solid phase carrier For nucleic acid hybridization, wash to remove unhybridized reactants; or mix and react the test substance with the test substance, hybridize with the first single-stranded nucleic acid immobilized on the solid phase carrier, and was...
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