High flux detection method and biochip based on nucleic acid address coding

A technology of address coding and detection methods, which is applied in the field of high-throughput detection methods and biochips based on nucleic acid address coding, can solve the problems of limiting the progress of proteomics research and the lack of biochips for protein detection methods, and achieve improved detection types, Reliable test results and good stability

Inactive Publication Date: 2012-05-02
广东朗源生物科技有限公司
View PDF8 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At this stage, there is a lack of an efficient, high-throughput protein detection method and corresponding biochip, which greatly limits the progress of proteomics research.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High flux detection method and biochip based on nucleic acid address coding
  • High flux detection method and biochip based on nucleic acid address coding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] A high-throughput detection method based on nucleic acid address coding, comprising the following steps:

[0030] 1) immobilizing the first single-stranded nucleic acid arrangement on a solid phase carrier;

[0031] 2) coupling a detection substance capable of specifically reacting with the analyte to a second single-stranded nucleic acid to obtain a detection body, wherein the second single-stranded nucleic acid is complementary to the first single-stranded nucleic acid;

[0032] 3) Mix and react the analyte with the analyte, then add the labeled secondary antibody, and continue the reaction to obtain the analyte-analyte-secondary antibody complex; combine the above complex with the first single-chain immobilized on the solid phase carrier For nucleic acid hybridization, wash to remove unhybridized reactants; or mix and react the test substance with the test substance, hybridize with the first single-stranded nucleic acid immobilized on the solid phase carrier, and was...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a high flux detection method and detection biochip based on nucleic acid address coding. According to the invention, the principle of specific complementation and hybridization of nucleic acid is ingeniously utilized to locate nucleic acid with different sequences at different areas of the chip; conjugates of complementary nucleic acid and proteins are located at corresponding positions on the biochip because of interaction among nucleic acid; therefore, disadvantages and deficiency of conventional protein chips are overcome, a whole reaction is carried out in a liquid environment, and since genetic coding is infinite, limitation of bead coding in liquid phase chips is overcome. Thus, the detection biochip provided in the invention combines the advantages of solid phase chips and liquid phase chips and enables a more accurate high flux detection reaction to be achieved.

Description

technical field [0001] The invention relates to a high-throughput detection method and a corresponding biological detection chip, in particular to a high-throughput detection method and a biological chip based on nucleic acid address coding. Background technique [0002] Biochip technology is a type of biological analysis and detection technology developed with the Human Genome Project in the mid-1990s. It has been widely used in gene sequencing, gene expression analysis, gene mutation and polymorphism detection, and the detection of microorganisms in food. , clinical medicine, etc., and has become one of the research hotspots in the scientific community (Lu Weihong, Fu Li, Zheng Qi, etc. Application and prospect of biochip technology [J]. Sensors and Microsystems, 2006, 25(6): 1-4). [0003] Biochips are divided into gene chips, protein chips, cell chips, and tissue chips according to the biological components detected and analyzed (Meilin, Song Shiyuan, Zha Zhongyong, etc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543C12Q1/68
Inventor 龚桂香
Owner 广东朗源生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap