Viewable gold nanorod test strip for rapid detection of ochratoxin A and preparation method thereof

A technology of ochratoxin and gold nanorods, which is applied to measuring devices, instruments, scientific instruments, etc., to achieve the effects of short response time, high sensitivity, and cheap instruments and equipment

Inactive Publication Date: 2012-05-02
王利兵 +2
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  • Abstract
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Problems solved by technology

At present, there is no report on the use of gold nanorods as mark

Method used

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  • Viewable gold nanorod test strip for rapid detection of ochratoxin A and preparation method thereof
  • Viewable gold nanorod test strip for rapid detection of ochratoxin A and preparation method thereof
  • Viewable gold nanorod test strip for rapid detection of ochratoxin A and preparation method thereof

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Embodiment Construction

[0034] To make gold nanorod immunoassay strips for ochratoxin A, it is first necessary to prepare the complete antigen coupled to ochratoxin A, which is used to prepare the corresponding detection line (T line) and antibody; and it is necessary to prepare ochratoxin A Labeled gold nanorods are used to prepare the corresponding binding pads loaded with gold nanorod-labeled antibodies; in addition, goat anti-mouse IgG antibodies are required to prepare the control line (line C).

[0035] 1) Synthesis of the whole antigen of ochratoxin A: Dissolve 1 mg OTA in 500 μL dioxane, place at -20°C, and pre-cool for 20 minutes. Under stirring in an ice bath, 0.5 μL of isobutyl chloroformate and 1 μL of tri-n-butylamine were sequentially added, mixed and reacted for 1 hour to obtain an activated acid anhydride solution. Dissolve 5 mg BSA or 4 mg OVA or 73 mg KLH in 500 μL of pure water to obtain a protein solution. The acid anhydride solution was slowly added dropwise to the protein solut...

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Abstract

A viewable gold nanorod test strip for rapid detection of ochratoxin A and a preparation method thereof relate to the technical field of ochratoxin A detection. The test strip is composed of a lining board and a sample pad, a gold nanorod tag binding pad, a coating film and a water absorption pad which are successively connected on the lining board. The gold nanorod tag binding pad is a glass fiber film or polyester film which carries gold nanorod labeled OTA antibody. The coating film contains an invisible detection line T line printed by ochratoxin A coupled protein solution and an invisible control line C line printed by goat anti mouse IgG solution. The sample pad of the test strip is inserted into a sample solution to be tested, a reaction is performed at room temperature for 15 min, and the result is determined according to the coloration condition of the detection line T and the control line C. According to the invention, ochratoxin A is detected based on the viewable gold nanorod test strip technology. The viewable gold nanorod test strip provided by the invention has characteristics of high sensitivity, short reaction time and cheap instrument and equipment, and is convenient and easy to use.

Description

technical field [0001] The invention discloses a visual gold nanorod test strip for rapid detection of ochratoxin A and a preparation method thereof, and relates to the technical field of ochratoxin A detection. Background technique [0002] Ochratoxins are toxic metabolites produced by several species of Aspergillus and Penicillium. In cold climates, the toxigenic mold is mainly P. viridicatum and in warm climates, the toxigenic mold is mainly A. Ochraceus. The basic chemical structure of ochratoxin is a derivative of isocoumarin linked to β-phenylalanine, and there are four compounds A, B, C, and D. The chemical structural formula of ochratoxin A (Ochratoxin A., abbreviated as OTA) is: [0003] [0004] There is no chlorine element in the molecular structure of ochratoxin B, ochratoxin C is the ethyl ester of ochratoxin A, and ochratoxin D is 4-hydroxy ochratoxin A. Considering the pollution rate, pollution level and toxicity to humans and animals of grains, OTA is a...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/531
Inventor 王利兵胥传来刘丽强
Owner 王利兵
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