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Ss-galactosidase derived from bacillus circulans

A technology of Bacillus circulans and galactosidase, applied in the direction of glycosylase, enzyme, bacteria, etc., can solve the problem of unclear coding activity of protein and other problems

Active Publication Date: 2012-05-09
AMANO ENZYME INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, two types of β-galactosidase genes (GenBank accession numbers (GenBank accession number) L03424 and L03425) derived from Bacillus circulans are registered in the gene database (GenBank), but only the gene sequence is reported for them, Not clear if encodes actual active protein

Method used

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  • Ss-galactosidase derived from bacillus circulans
  • Ss-galactosidase derived from bacillus circulans
  • Ss-galactosidase derived from bacillus circulans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0089] 1. Purification of β-galactosidase from Bacillus circulans

[0090] (a) β-galactosidase activity assay

[0091] In the following purifications, β-galactosidase activity was measured by method (i) using 2-nitrophenyl β-D-galactopyranoside (ONPG) as a substrate and method (i) using lactose as a substrate Method (ii) These two types are measured. All were carried out according to the method described in Non-Patent Document 1. In addition, the protein concentration is represented by the absorbance at 280 nm.

[0092] i) ONPG method

[0093] 1.98 ml of 100 mM phosphate buffer (pH 6.0) containing 0.245% ONPG was preheated at 40° C. for 10 minutes. 20 μl of the sample was added thereto, and after reacting at 40° C. for 10 minutes, 2.0 ml of a 10% sodium carbonate solution was added to stop the reaction. The absorbance at 420 nm of the reaction solution was measured, and the activity when 1 μmol of 2-nitrophenol was produced per minute was regarded as 1 U to calculate the ...

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Abstract

Disclosed is a novel ss-galactosidase. Specifically disclosed are a ss-galactosidase derived from Bacillus circulans and a gene for the ss-galactosidase. The ss-galactosidase can be used, for example, in the production of milk, dairy products, fermented dairy products, galacto-oligosaccharides or supplements for foods.

Description

technical field [0001] The present invention relates to beta-galactosidases. Specifically, it relates to a novel β-galactosidase isolated from Bacillus circulans, a gene thereof, uses thereof, and the like. The β-galactosidase of the present invention is useful, for example, in the production of low-lactose milk, in the production of galactooligosaccharides as a growth factor for bifidobacteria in the intestines, or as an effective drug for lactose intolerant patients or supplements. Element. This application claims priority based on Japanese Patent Application No. 2009-136735 for which it applied on June 5, 2009, and takes in the whole content of this patent application here by reference. Background technique [0002] β-galactosidase (EC3.2.1.23) is an enzyme that hydrolyzes β-D-galactosidic bonds to release D-galactose, and generally exists widely in microorganisms, animals and plants. The alias of β-galactosidase is also called lactase, and it is used as an enzyme for ...

Claims

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Application Information

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IPC IPC(8): C12N15/09A23L1/30A61K38/46A61P3/02C12N1/15C12N1/19C12N1/21C12N9/38
CPCC12N9/2468A23L1/034A61K38/47C12N9/2471C12Y302/01023A23C9/1206A23L29/06A61P1/14A61P3/00A61P3/02C12N15/63G01N2333/938G01N2333/94
Inventor 片濑彻星由纪子长屋美穗山口庄太郎箕田正史中西一弘
Owner AMANO ENZYME INC
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