Supercharge Your Innovation With Domain-Expert AI Agents!

Method for preparing soluble globin

A globin and hemoglobin technology, applied in the fields of biotechnology and agriculture, can solve problems such as large gaps, backward processes, and environmental pollution

Inactive Publication Date: 2012-05-23
SHANGHAI GENON BIOLOGICAL PROD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Second, serious environmental pollution
The direct discharge of the remaining blood components after removing the blood clot, as well as the outdated pressing and sun exposure process can cause environmental pollution due to the sewage and rancidity formed during the production process
[0006] Third, product efficacy and availability are low
Therefore, there is still a certain gap in the comprehensive utilization and development of animal blood in China. The technology is relatively backward and the products are relatively single. In particular, the development of functional products using animal blood has a greater gap.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing soluble globin
  • Method for preparing soluble globin
  • Method for preparing soluble globin

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0045] The invention discloses a preparation method of globin, comprising the steps of:

[0046] (a) breaking the cell membrane of the blood cells in water to obtain a membrane-ruptured hemoglobin liquid, and centrifuging to remove the cell membrane to obtain a membrane-removed hemoglobin liquid;

[0047] (b) mixing and stirring the removed hemoglobin solution with the inorganic acid to obtain the heme and globin mixed solution I (the inorganic acid is used to adjust the pH value to 1-4);

[0048] (c) mixing and stirring the mixed solution I with activated carbon to obtain the mixed solution II;

[0049] (d) Microfiltration of the mixed solution II at least once (1-5 times, preferably 2-3 times) to obtain the permeate; the molecular weight of the membrane used for microfiltration is 10 ± 20,000 (preferably 10 ± 1 ten thousand) unit;

[0050] (e) Desalting the permeate by nanofiltration to obtain a retentate V; the molecular weight of the membrane used in the nanofiltration i...

Embodiment approach

[0070] As a preferred embodiment of the present invention, a method for preparing globin is disclosed, the method comprising the steps of:

[0071] (1) Mix blood cells with water three times the volume of blood cells, and stir for 1-3 hours to obtain hemoglobin liquid with ruptured membranes; the blood cells are from livestock; the blood cells are fresh and healthy blood cells; the water is pure water ;

[0072] (2) Centrifuge the membrane-ruptured blood cell liquid to remove the cell membrane; the centrifugation adopts a disc centrifuge with a rotating speed of 8000 rpm, and the discharge from the slag outlet at the lower end is the cell membrane;

[0073] (3) Mix the removed hemoglobin solution with inorganic acid, and stir at 30-40°C for 6-12 hours to obtain heme and globin mixed solution I; the inorganic acid can be citric acid, hydrochloric acid, etc., and the amount added depends on the pH depends.

[0074] (4) Mix the mixed solution I with activated carbon, stir at 40...

Embodiment 1

[0083] Embodiment 1, the preparation of soluble globin 1

[0084] (0) Separation of porcine anticoagulated whole blood to obtain plasma and blood cells respectively. Blood cells are used to produce globin. After the anticoagulated whole blood is centrifuged by a tubular centrifuge (12000 rpm), the obtained light liquid is plasma, and the heavy liquid is blood cells, which are used to prepare soluble globin.

[0085] (1) Mix 2 tons of blood cells with 6 tons of pure water, and stir for 2 hours to obtain a membrane-ruptured hemoglobin liquid; the blood cells are fresh and healthy blood cells; the water is pure water;

[0086] (2) Centrifuge the 8 tons of hemoglobin liquid that has ruptured the membrane with a disc centrifuge to remove the cell membrane; the centrifugal speed is 8000 rpm, and the discharge from the slag outlet at the lower end is the cell membrane;

[0087] (3) 15 kg of hydrochloric acid (concentration 38% (w / v)) was mixed with 42 kg of pure water (the final co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing soluble globin. Based on activated carbon adsorption technology and microfiltration technology, purified globin is separated from blood corpuscles. The large-scale production of globin can be realized, the method is convenient and quick, the cost is low, and the yield of a product is high. The method provided by the invention is favorable for recovery and utilization of blood resources, the waste of resources is avoided, and the environmental pollution is effectively reduced.

Description

technical field [0001] The invention belongs to the fields of biotechnology and agriculture; more specifically, the invention relates to a preparation method of soluble globin. Background technique [0002] Recently, the scale of my country's slaughtering industry is expanding day by day. While slaughtering livestock and poultry to produce meat, a large amount of by-products such as feathers, blood, viscera, and hoof shells are produced. If these huge resources cannot be used or disposed of in a reasonable and timely manner, they will not only cause environmental pollution , will also lead to a serious waste of resources. [0003] The annual output of animal blood resources of livestock and poultry by-products in my country is nearly 2.3 million tons. At present and in the past for quite a long time, most of the blood of livestock and poultry animals is collected for processing whole blood meal, and at the same time, a considerable part of blood is discharged randomly due to...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C07K1/34C07K1/14
Inventor 成国祥于伟江国永潘勇
Owner SHANGHAI GENON BIOLOGICAL PROD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com