Preparation method of porous chitosan scaffold
A technology of porous chitosan and chitosan, which is applied in the field of preparation of porous chitosan scaffolds, can solve the problem that chitosan scaffold plasticity and microstructure connectivity are difficult to meet the requirements of the field of cell culture technology research, and the preparation method is limited. Limitation and other issues, to achieve the effect of easy molding, good connectivity, and good connectivity
Inactive Publication Date: 2013-05-22
UNIV OF SHANGHAI FOR SCI & TECH
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Problems solved by technology
However, its preparation method is often limited to the commonly used freeze-drying method
The chitosan scaffold prepared by the traditional freeze-drying method is difficult to meet the requirements of the technical research field of cell culture in terms of plasticity, microstructure and connectivity between pores.
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Embodiment 1
[0043] A kind of preparation method of porous chitosan support, comprises the steps:
[0044] (1) Preparation of solution
[0045] ①, preparation of chitosan acid solution dissolved in ammonium chloride
[0046] First measure 1ml of acetic acid and add it to a 100ml volumetric flask to make a constant volume to prepare a 1% (V / V) acetic acid solution, weigh 0.2g of ammonium chloride, add it to the 1% (V / V) acetic acid solution and stir evenly, then Weigh 3.0g chitosan and add it into 1% (V / V) acetic acid solution dissolved with ammonium chloride;
[0047] ②. Prepare 1% (v / v) glutaraldehyde solution
[0048] Measure 1ml of pure glutaraldehyde and add distilled water to make up to 100ml, store at 4°C for later use;
[0049] ③, preparation of 5% sodium borohydride solution
[0050] Weigh 5.0g of sodium borohydride and add it to constant volume in a 100ml volumetric flask to obtain a 5% sodium borohydride solution;
[0051] ④, prepare 10% (W / V) sodium hydroxide soluti...
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Abstract
The invention discloses a preparation method of a porous chitosan scaffold, which is prepared by: taking chitosan as a raw material and ammonium chloride as a pore forming agent, and preparing a chitosan acid solution first, injecting the solution into a die for first freeze drying so as to obtain an ammonium chloride-containing chitosan scaffold, then immersing the scaffold in a sodium hydroxidesolution for 20min and cleaning it with distilled water for three times so as to obtain a chitosan scaffold, which is finally subjected to second freeze drying, thus obtaining a porous chitosan scaffold, which has a pore size of 20-60, porosity of 90%, and good connectivity among pores. The preparation method of the invention has the advantages of simple operation and simple process, and the obtained porous chitosan scaffold can be used for the technical research on cell culture.
Description
technical field [0001] The invention belongs to the field of preparation of porous materials, in particular to a method for preparing a porous chitosan support. Background technique [0002] At present, whether it is laboratory or industrial production, the cell culture microcarriers used are mainly solid Cytodex series carriers, which belong to the monolayer monolayer culture method. In vivo, the growth and development process of tissues and cells is carried out under three-dimensional internal environment conditions, and the mutual signal transmission between cells and cell matrix and cells has an important impact on the regulation of cell proliferation and differentiation. Conventional in vitro single The multilayer culture method cannot provide the three-dimensional environmental conditions required for the normal growth and development of tissues, so the preparation of three-dimensional porous scaffolds is of great significance. [0003] In recent years, in the researc...
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IPC IPC(8): C12N5/02
Inventor 李琳刘宝林韩宝三李维杰
Owner UNIV OF SHANGHAI FOR SCI & TECH