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Method for producing trivalent inactivated vaccine against Haemophilus parasuis infection
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A technology of Haemophilus suis and inactivated vaccines, applied in the field of veterinary biological products, can solve problems such as losses in pig farming
Active Publication Date: 2013-07-17
YEBIO BIOENG OF QINGDAO
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Problems solved by technology
With the development of the pig industry, Haemophilus parasuis has become a new bacterial infectious disease that seriously endangers the pig industry in recent years. It is distributed worldwide and has been confirmed to exist and prevail in most pig farms in my country. It can affect pigs aged 2 weeks to 4 months, but it mainly occurs around weaning and piglet stage. It is common in pigs aged 5-8 weeks. The incidence rate is generally 10-15%, and the mortality rate can reach more than 50% in severe cases , causing huge losses to my pig industry
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Embodiment 1
[0100] 1. Seed Preparation for Production
[0101] (1) Propagation and identification of primary seeds Streak inoculation of freeze-dried strains of YBH04, YBH05, and YBH13 strains on TSA agar plates, in 5% to 10% CO 2 Under these conditions, culture at 37°C for 24-48 hours, pick typical colonies or lawns, inoculate in 100ml TSB broth, and culture at 37°C for 24-48 hours with shaking. Take 2ml of the bacterial solution and pass the pure inspection as the first-class seeds. Store at -20°C for no more than 1 month; store at -70°C for no more than 3 months (see Table 13).
[0102] Table 13 Level 1 Seeds
[0103] Strain number
Seed Quantity(ml)
use medium
Colony growth
HPS YBH04 strain
100
TSA
Round, smooth and moist, colorless and transparent colonies
HPS YBH05 strain
100
TSA
Round, smooth and moist, colorless and transparent colonies
[0122] Dosage form water-in-oil type. Take a clean straw, suck 2ml of the vaccine and drop it on the surface of cold water in the form of oil droplets without spreading.
[0123] Stability Draw 10ml of vaccine into a centrifuge tube and centrifuge at 3000r / min for 15min without stratification.
[0124] Viscosity Use a 1ml straw (the inner diameter of the lower mouth is 1.2mm, and the inner diameter of the upper mouth is 2.7mm) to draw 1.0ml of vaccine at about 25°C, let it flow out vertically and naturally, and record the time required for the outflow of 0.4ml to be 4.9 seconds.
[0125] (2) Sterility test is carried out according to the method of "Chinese Veterinary Pharmacopoeia", and the growth is sterile.
[0126] (3) Safety inspection Take 5 healthy susceptible piglets aged 4-5 weeks, inject the vaccine into the neck muscles, 4ml / head, observe for 14 days, and there is...
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Abstract
The invention relates to a method for producing a trivalent inactivated vaccine against Haemophilus parasuis infection. The method comprises the following steps of: screening Haemophilus parasuis type 4 strain YBH04, Haemophilus parasuis type 5 strain YBH05 and Haemophilus parasuis type 13 strain YBH13 from predominant prevalence strains, which have good immunogenicity and serve as vaccine strains; inoculating to appropriate culture media for culture respectively; collecting strain suspensions; inactivating with a formaldehyde solution; adding mineral oiladjuvant; and mixing and emulsifying.The vaccine is used for preventing infection of Haemophilus parasuis type 4, Haemophilus parasuis type 5 and Haemophilus parasuis type 13 in pigs, and has the advantages of good safety, high immunization efficacy and long duration of immunity.
Description
technical field [0001] The invention relates to a production method of a trivalent inactivated vaccine of Haemophilus parasuis, belonging to the field of veterinary biological products. Background technique [0002] Haemophilus parasuis disease, also known as Glasser's disease, is a disease characterized by respiratory symptoms, lameness, pleurisy, pericarditis, peritonitis, arthritis and meningitis caused by Haemophilus parasuis Porcine respiratory infectious diseases characterized by such as. With the development of the pig industry, Haemophilus parasuis has become a new bacterial infectious disease that seriously endangers the pig industry in recent years. It is distributed worldwide and has been confirmed to exist and prevail in most pig farms in my country. It can affect pigs aged 2 weeks to 4 months, but it mainly occurs around weaning and piglet stage. It is common in pigs aged 5-8 weeks. The incidence rate is generally 10-15%, and the mortality rate can reach more th...
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