Capillary electrophoresis electrochemiluminescence detection method for arecoline in traditional Chinese medicine betelnut extract

A technology of capillary electrophoresis and luminescence detection, which is used in electrical excitation analysis, measurement devices, scientific instruments, etc., can solve problems such as environmental pollution, and achieve the effects of improving detection sensitivity, simple and fast operation, and simple operation.

Inactive Publication Date: 2012-06-20
CHANGCHUN INST OF TECH
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Problems solved by technology

However, because high-performance liquid chromatography uses organic mobile phases to separate the components to be tested, pollution to the environment is one of the main problems of this technology. For this reason, people have been working hard to explore new technologies for the separation and analysis of active components of traditional Chinese medicine that are environmentally friendly, efficient and sensitive.

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  • Capillary electrophoresis electrochemiluminescence detection method for arecoline in traditional Chinese medicine betelnut extract

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Embodiment 1

[0055] The invention provides a capillary electrophoresis electrochemiluminescence detection method for arecoline in the Chinese medicine betel nut extract, the steps and conditions are as follows:

[0056] 1.1 Preparation of the sample to be inspected

[0057] 1.1.1 Preparation of arecoline standard solution

[0058] Dissolve the arecoline standard substance in secondary water to obtain a concentration of 5.0×10 -3 The arecoline standard solution of mol / L;

[0059] 1.1.2 Preparation of related solutions of betel nut extract

[0060] 1.1.2.1 Preparation of betel nut extract solution

[0061] Take 0.3g of the traditional Chinese medicine areca nut powder, use 2.0mL of secondary water to ultrasonically extract in the ultrasonic tank for 30min, repeat the extraction operation 3 times, combine the extracts, and filter with a 0.45μm filter membrane to obtain the betel nut extract solution;

[0062] 1.1.2.2 Preparation of betel nut extract dilution solution

[0063] Add seconda...

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Abstract

The invention provides a capillary electrophoresis electrochemiluminescence detection method for arecoline in a traditional Chinese medicine betelnut extract. The method applies capillary electrophoresis ruthenium pyridine electrochemiluminescence analysis technology to the detection of arecoline in the traditional Chinese medicine betelnut extract for the first time, and has the advantages that the operation is simple, the sensitivity is high, the detection limit of arecoline is 5*10<-9> mol / L, the separation function is strong, the pollution is avoided, and the field amplified injection is realized and the separation selectivity of arecoline and coexisting media in the betelnut extract is improved by using ionic liquid BMImBF4 in a buffer. Electrochemiluminescence reagent ruthenium pyridine is reversible in reaction and high in photon yield, thus the reagent consumption is greatly reduced and the detection cost is lowered. The analysis method is green, environmentally friendly and low in sample consumption. The analysis can be finished by adopting a capillary electrophoresis electrochemiluminescence comprehensive analyzer and chemical reagents which are common in laboratory and requiring nonoliter-scale injection volume.

Description

technical field [0001] The invention belongs to the technical field of capillary electrophoresis electrochemiluminescence analysis, in particular to a method for detecting arecoline in a traditional Chinese medicine betel nut extract by capillary electrophoresis electrochemiluminescence. Background technique [0002] Betel nut is one of the famous and precious "four southern medicines" in my country. It is mainly used to treat diseases such as worm accumulation, food accumulation, qi stagnation, dysentery, ascariasis, and glaucoma. Excessive consumption is prone to poisoning, which can stimulate the digestive tract to produce vomiting, increase gland secretion, and affect the central nervous system and urinary system. Arecoline is an important bioactive component in betel nut. The separation and analysis of arecoline in betel nut extract is an important index for evaluating the quality of arecoline in traditional Chinese medicine. Due to the low content of active ingredient...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/66G01N27/447
Inventor 高英向前张敬天杨明唐娟王琨琦吴仙花杨焕欣
Owner CHANGCHUN INST OF TECH
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