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Electrochemical biological sensor and preparation method and application thereof

A biosensor, sensor technology, applied in scientific instruments, instruments, material analysis by electromagnetic means, etc., can solve problems such as low precision, cumbersome preparation process, affecting device performance, etc. The effect of sensitivity

Active Publication Date: 2014-03-12
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above preparation processes are very cumbersome, with poor controllability, low precision, and high cost, which directly affect the performance of the device; since electrochemical biosensors are widely used in the field of medical testing, this will significantly increase the economic burden of medical testing for patients

Method used

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  • Electrochemical biological sensor and preparation method and application thereof
  • Electrochemical biological sensor and preparation method and application thereof
  • Electrochemical biological sensor and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1, preparation of three-electrode electrochemical biosensor

[0054] 1) Using Microfab jetlab II printer according to image 3 The designed three-electrode electrochemical biosensor, on the substrate made of polyvinyl chloride (PVC) film produced by the American PPG company, dissolves the materials constituting the conductive layer of the working electrode, the conductive layer of the counter electrode and the conductive layer of the reference electrode in a volumetric After using a solvent composed of water and ethylene glycol at a ratio of 1:1, inkjet print it on the PVC substrate according to the preset shape and position, and dry it in a muffle furnace at 80°C for 15 minutes to obtain the working electrode conductive layer and the counter electrode in sequence. conductive layer and reference electrode conductive layer;

[0055] Wherein, the ink materials that constitute the working electrode conductive layer, the counter electrode conductive layer and the r...

Embodiment 2

[0078] Embodiment 2, preparation single-electrode electrochemical biosensor

[0079] 1) Use Epson C110 printer according to Figure 4 The designed single-electrode electrochemical biosensor and Figure 7 The schematic diagram of ink cartridge distribution is prepared by printing. First, on the substrate composed of polyimide (PI) film produced by DuPont Teijin, the material that constitutes the conductive layer of the working electrode is dissolved in water and ethylene glycol with a volume ratio of 1:1. After the solvent is formed, it is inkjet printed on the PVC substrate according to the preset shape and position, and is dried in a muffle furnace at 300°C for 30 minutes to obtain the working electrode conductive layer in sequence;

[0080] Wherein, the ink material constituting the conductive layer of the working electrode is a metal copper conductive ink (NovaCentrix.Inc., Metalon ICI-003) with a particle size of 50nm, and the mass percent concentration of the conductive ...

Embodiment 3

[0096] Embodiment 3, preparation double-electrode electrochemical biosensor

[0097] 1) Use HP DeskJet D1668 printer according to Figure 5 The designed dual-electrode electrochemical biosensor is based on the substrate made of polyvinyl chloride (PVC) printing film produced by Jiangsu Huaxin Company, and the materials that constitute the conductive layer of the working electrode and the conductive layer of the counter electrode are dissolved in a volume ratio of 1. : 1 solvent composed of water and ethylene glycol, inkjet printing on the PVC substrate according to the preset shape and position, and drying in a muffle furnace at 150°C for 10 minutes to obtain the working electrode conductive layer and the counter electrode conductive layer in sequence;

[0098] Wherein, the materials constituting the conductive layer of the working electrode and the conductive layer of the counter electrode are gold conductive ink (NanoMas Technologies, Inc., NTG05) with a particle size of 100...

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Abstract

The invention discloses an electrochemical biological sensor and a preparation method and an application of the sensor. The method comprises the steps of: preparing component materials (except a substrate) of the sensor into a solution or a nanomaterial emulsion as ink, printing the components layer by layer on the surface of the substrate by inkjet printing technology, and finally assembling the biological sensor with electrochemical response. Compared with the electrochemical biological sensor prepared by conventional physical etching and silk screen printing method, the electrochemical biological sensor provided by the invention is convenient and rapid to operate, has strong controllability and low preparation cost, and is convenient for mass production.

Description

technical field [0001] The invention relates to an electrochemical biosensor and its preparation method and application. Background technique [0002] Electrochemical biosensors refer to immobilized organisms themselves (cells, organelles, tissues, etc.) or their components (enzymes, antigens, antibodies, hormones, etc.) as sensitive elements, electrodes as signal conversion elements, and potential or current as detection signal sensor. Due to the advantages of low detection limit, high sensitivity, easy operation and low cost, electrochemical biosensors have been widely used in clinical testing, environmental analysis, food safety, drug analysis and other fields in recent years (Handbook of Biosensors and Biochips, Chem.Rev. 2008, 108, 2646-2687). [0003] Currently, the main preparation methods of electrodes for electrochemical biosensors are physical etching and screen printing. In the technical scheme disclosed in the publication number CN1584575, the title of the inv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 邓萌萌张兴业张志良辛智青宋延林
Owner INST OF CHEM CHINESE ACAD OF SCI