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In-vitro simulation detection method for bioavailability of nicotine in tobacco gum base product

An in vitro simulation and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult experiments, control of human body damage, etc., and achieve the effects of good repeatability, easy promotion, and easy operation

Active Publication Date: 2012-06-20
ZHENGZHOU TOBACCO RES INST OF CNTC +1
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Problems solved by technology

The experimental results of this method are easily affected by the individual physiological differences of the volunteers and the habit of consuming tobacco products. The experiment is not easy to control and has great harm to the human body.

Method used

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  • In-vitro simulation detection method for bioavailability of nicotine in tobacco gum base product
  • In-vitro simulation detection method for bioavailability of nicotine in tobacco gum base product
  • In-vitro simulation detection method for bioavailability of nicotine in tobacco gum base product

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Embodiment 1

[0034] Test sample: Nicorette Classical 2mg Gum (UK). The pH of the artificial saliva was 6.4, the initial volume of artificial saliva in the small bowl of chewing noodles was 10 mL, the flow rate of the peristaltic pump was 20 mL / min, and the test was carried out for 60 min. The nicotine release liquid and the supplement of fresh artificial saliva were extracted every 5 minutes, and the volume of extraction and supplement of artificial saliva was 5 mL; the system automatically stopped at 60 min, and the peristaltic pump pumped out the last release liquid. Take out the residual sample, put it into a 50 mL Erlenmeyer flask, add 5.0 mL of n-hexane, cover the stopper, and shake it on a shaker for 15 min. After the gel matrix sample is completely dissolved, add 20 mL of mixed phosphate buffer solution to it (pH = 6.86), placed on the shaker again for 30 min, and separated to remove the buffer solution layer.

[0035] The detection of nicotine in the release liquid and the residua...

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Abstract

The invention discloses an in-vitro simulation detection method for the bioavailability of nicotine in a tobacco gum base product. The in-vitro simulation detection method for the bioavailability of the nicotine in the tobacco gum base product is characterized by comprising specific steps of: (1) releasing and collecting nicotine in vitro; (2) performing quantitative analysis on the content of the nicotine in collected liquid and residual samples; and (3) drawing an in-vitro nicotine bioavailability curve. The method has the advantages that: the chewing behavior of the mouth of a person can be simulated well by using a self-designed pressure changing type simulation chewing machine; for the rationally prepared artificial saliva and the continuous updating of the artificial salvia in a chewing cavity, nicotine in the tobacco gum base product can be released fully; as nicotine release liquid can be collected in real time for detection and the test result is close to the true condition of the product used by a person, the specific data support can be provided for judging the safety of the tobacco gum base product and performing quality regulation, control and research. The method hasthe advantages of good repeatability, high sensitivity and short detection period, and is suitable for rapid analysis of samples in industries, such as tobacco, food, medicines and the like.

Description

technical field [0001] The invention relates to an in vitro simulation detection method of nicotine bioavailability in tobacco gum-based products. The method can provide technical support for safety evaluation and quality control of tobacco gum-based products. Background technique [0002] With the continuous deepening of research on smoking and health, some scientific research has shown that most of the harmful substances in cigarettes are produced by tobacco combustion and enter the human body through smoking. Passive smoking of non-smoking people is also mainly caused by cigarettes. Smoking is increasingly subject to criticism and restrictions from all walks of life. Adopting and popularizing effective smoking cessation methods will have positive significance for smoking ban and control in public places. [0003] Smokers' dependence on cigarettes stems from their dependence on nicotine. If you quit smoking simply by willpower, the body will suddenly lack nicotine, which ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 张文娟李鹏张建勋慎龙舞曾波宗永立黄卫东
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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