Preparation method of water-soluble active polysaccharide in boletus edulis, and application thereof

A technology of delicacy boletus and active polysaccharides, which is applied to the preparation of water-soluble active polysaccharides in delicacy boletus thallus, the field of application of delicacy boletus polysaccharides in antibacterial, can solve the problem of long growth cycle, fruit body active substances Problems such as low yield and difficult cultivation

Inactive Publication Date: 2012-06-27
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some studies focus on the domestication of bacteria and artificial cultivation of fruiting bodies, but due to insufficient artificial cultivation techniqu

Method used

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  • Preparation method of water-soluble active polysaccharide in boletus edulis, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of preparation method of water-soluble active polysaccharide in boletus thalline, its steps are:

[0038] A Inoculate Boletus deliciosa (No. ACCC50559, provided by China Agricultural Microorganism Culture Collection Center) on a solid slant medium, and culture it at a constant temperature of 25°C for 6 days to obtain slant seeds. Solid slant medium (%): potato 20, glucose 2.0, yeast powder 1.4, agar 2, peptone 0.6, dihydrogen phosphate 0.1, zinc sulfate heptahydrate 0.05.

[0039] B Take 4-5 loops of the cultured slant and inoculate it into the primary liquid seed medium, add 10 glass beads in a 250ml Erlenmeyer flask with a liquid volume of 100ml, and incubate at 120r / min at 25°C for 60 hours. Each component and its content in the medium are (%): 20% potato, 2.0% glucose, 1.4% yeast powder, 0.6% peptone, 0.1% methyl dihydrogen phosphate, and 0.05% zinc sulfate heptahydrate.

[0040] C Inoculate the first-level liquid seeds after 60 hours of cultivation into the...

Embodiment 2

[0046] A kind of preparation method of water-soluble active polysaccharide in boletus thalline, its steps are:

[0047] Embodiment 2 steps A-E are the same as steps A-E in embodiment 1

[0048] F Take an appropriate amount of crushed boletus mycelium, add 50 times the amount of deionized water, and extract at 53 or 54 or 55 or 56°C for 2 hours. Repeat 3 times, combine the 3 extracts, and centrifuge the extract at 7000rpm for 8min. The filtrate was concentrated at 54°C under reduced pressure (0.08-0.09MPa) to 1 / 3 of the original volume, and 3 times the volume of 95% (v / v) ethanol was added to the concentrated extract with stirring to precipitate the polysaccharide in the concentrated extract , collect the crude polysaccharide precipitate of boletus mycelium; add deionized water to the crude polysaccharide precipitate to dissolve it, make it fully redissolved, use the sevag method to remove protein, and repeat until no protein is detected. After concentrating under reduced pre...

Embodiment 3

[0051] A kind of preparation method of water-soluble active polysaccharide in boletus thalline, its steps are:

[0052] Steps A-C of embodiment 3 are the same as steps A-C in embodiment 1

[0053] D Inoculate the secondary liquid seeds after 40 hours of cultivation into a 250mL Erlenmeyer flask for 72 hours of fermentation at an inoculum volume of 5%. The components of the fermentation medium are (%): yeast extract 1, peptone 2, and glucose 2.

[0054] E The mycelium is separated from the fermentation broth, rinsed with clean water, dried at 50°C, and ground to obtain mycelium dry powder.

[0055] F Take an appropriate amount of crushed boletus mycelium, add 50 times the amount of deionized water, and sonicate at 52 or 53 or 54 or 55 or 56°C for 8.5 minutes. Repeat 3 times, combine the 3 extracts, and centrifuge the extract at 7000rpm for 8min. The filtrate was concentrated at 54°C under reduced pressure (0.08-0.09MPa) to 1 / 3 of the original volume, and 3 times the volume o...

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Abstract

The invention discloses a preparation method of water-soluble active polysaccharide in boletus edulis, and an application thereof. The method comprises steps that: (1) boletus edulis is inoculated to a solid slant cultivation medium, such that slant seeds are obtained; (2) the cultivated slant is inoculated to a primary liquid seed cultivation medium, and is cultivated; (3) the cultivated primary liquid seeds are inoculated to secondary liquid seeds, and are cultivated; (4) the cultivated secondary liquid seeds are inoculated and fermented; (5) mycelium is separated from a fermentation broth, such that mycelium dry powder is obtained; (6) the mycelium dry powder is subject to ultrasonic lixiviation, such that liquid is concentrated; boletus edulis polysaccharide crude precipitate is collected; the boletus edulis polysaccharide crude precipitate is dissolved by using water; free protein is removed; the solution is concentrated, and is precipitated by using ethanol; the precipitate is dried, such that a protein-removed boletus edulis intracellular polysaccharide precipitate is obtained. The boletus edulis water-soluble active intracellular polysaccharide is applied in the preparations of medicines used for treating or preventing bacteria. The method provided by the invention is advantaged in simple method, convenient operation, low temperature, short time, and high efficiency. The prepared polysaccharide has good antibacterial property.

Description

technical field [0001] The invention belongs to the field of bioengineering and technology, and more specifically relates to a preparation method of water-soluble active polysaccharide in boletus thallus. It also relates to the use of a delicious boletus polysaccharide in antibacterial. The polysaccharide can be widely used as a raw material of antibacterial agents in industries such as medicine and food. Background technique [0002] Delicious boletus, also known as bigfoot mushroom and white boletus, is a species of Basidiomycotina, Phylomycetes, Agaricaceae, and Boletaceae, mainly distributed in Henan, Taiwan, Heilongjiang, Sichuan, and Guizhou in China , Yunnan, Tibet, Inner Mongolia, Fujian and other regions. It is an edible fungus, which is rich in nutrients and contains various vitamins, amino acids, polysaccharides and minerals. The bacterium has the effects of lowering blood fat and improving immunity. In addition, the inhibition rate of its water extract on mous...

Claims

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Application Information

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IPC IPC(8): C12P19/04C08B37/00A61K31/715A61P31/04C12R1/645
CPCY02A50/30
Inventor 张华山王伟平陈维
Owner HUBEI UNIV OF TECH
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