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Wavelength modulation surface plasmon resonance detector based on laser confocal imaging

A technology of laser confocal and surface plasmon, which is applied in the directions of measuring devices, scattering characteristics measurement, and material analysis through optical means, can solve the problems of inconvenient use, complicated operation, expensive equipment, etc., and achieves wide application range, convenient use, Simple operation effect

Inactive Publication Date: 2012-06-27
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] (1) A chopper and a lock-in amplifier are required, the equipment is expensive and the cost is high, which is not conducive to popularization and use;
[0007] (2) The operation is complicated and inconvenient to use

Method used

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  • Wavelength modulation surface plasmon resonance detector based on laser confocal imaging
  • Wavelength modulation surface plasmon resonance detector based on laser confocal imaging

Examples

Experimental program
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Embodiment 1

[0037] Such as figure 1 As shown, the wavelength modulation surface plasmon resonance detection device based on laser confocal imaging provided in this embodiment includes a rectangular prism 1, a substrate 2 coated with a reflective film, a constant temperature flow cell 3, an optical path mechanism 4, a syringe pump 5, an inverted Laser scanning confocal microscope 6, spectrometer 7, processing system 8, three-dimensional adjustment mechanism (not shown in the figure) and angle adjustment mechanism (not shown in the figure).

[0038] The upper end of the constant temperature flow cell 3 is open, and its two sides are respectively provided with a liquid inlet and a liquid outlet. The substrate 2 is arranged on the constant temperature flow cell 3 such that the upper end of the constant temperature flow cell 3 is closed, and the side of the substrate 2 coated with a reflective film faces into the constant temperature flow cell 3 . The prism 1 is arranged on the substrate 2 , ...

Embodiment 2

[0047] The emission film on the surface of the substrate 2 adopts such as Au (45nm) / [TiO 2 (20nm) / ITO(20nm)] 4 / Au(20nm) composite film or Au(25nm) / [TiO 2 (20nm) / SiO2 2 (20nm)] 2 / Au (30nm) composite film, the others are the same as in Example 1. Compared with Example 1, the sensitivity and detection accuracy of the device described in this example can be improved by 1-2 orders of magnitude.

Embodiment 3

[0049] The rectangular prism 1 and the substrate 2 are made of, for example, sapphire glass with a refractive index of 1.81, and the others are the same as in the first embodiment. The device described in this embodiment expands the detection range of the refractive index of the sample to 1.33-1.76.

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Abstract

The invention discloses a wavelength modulation surface plasmon resonance detector based on laser confocal imaging. The detector comprises a rectangular prism (1), a substrate (2) with one surface plated with a reflective film, a constant temperature flow-through cell (3), optical path mechanism (4), syringe pump (5), inversion laser scan confocal microscope (6), a spectrometer (7) and a processing system (8). The optical path mechanism (4) comprises a white light source (41), a light beam shaping mirror set (42), a polarizer (43), a focusing lens (44) and a fiber receiving head (45). The white light source (41), the light beam shaping mirror set (42) and the polarizer (43) are successively arranged on an optical path along incident light. The focusing lens (44) and the fiber receiving head (45) are successively arranged on an optical path along emitting light. The detector has low cost and wide scope of application, and is simple operated and convenient for usage. The detector can detect refractive index change reaching 2*10<-5> and has detection sensitivity reaching 10-11g / ml and image resolution reaching 2 mum.

Description

technical field [0001] The invention relates to the technical field of devices for detecting biomolecular reactions, in particular to a wavelength-modulated surface plasmon resonance detection device based on laser confocal imaging. Background technique [0002] In life science, the interaction between biomolecules is a basic life phenomenon, and it is also one of the major topics of modern life science research. There are many traditional methods to study the interaction between biomolecules, such as radioimmunoassay, enzyme-linked immunoassay and label-tracing method. However, due to the involvement of different types and different contents of cells or biomolecules, and the complex interactions between cells or biomolecules, it is difficult for the above-mentioned traditional methods to accurately obtain relevant transfer information. On the other hand, the increasing amount of new protein and DNA sequence data also urgently requires new methods that can accurately and qu...

Claims

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Application Information

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IPC IPC(8): G01N21/55G01N21/552
Inventor 张洪艳汪鹏飞
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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