Composition containing gallocatechin epicatechol gallate and vincristine and capable of inhibiting proliferation of tumor cells
An epigallocatechin and tumor cell proliferation technology, which is applied in the fields of medicine and genetic engineering, can solve the problems such as the combined use of EGCG and Vincristine, which has not been seen, and achieves the advantages of enhancing the anti-tumor effect, reducing the treatment cost, reducing the dosage and the cost of the drug. Effect
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Embodiment 1
[0031] Example 1 Synergistic effect of EGCG and Vincristine
[0032] In SK-hep-1 cells, the synergistic effect of EGCG and Vincristine is more significant. This example further verified the combined effect of EGCG and Vincristine, and the results proved that the CI values were all less than 1 within a certain concentration range, and the two drugs had a synergistic effect. The results showed that the combined use of EGCG and Vincristine could improve the antitumor effect of Vincristine.
[0033] Table 1
[0034]
Embodiment 2
[0035] Example 2 EGCG has a synergistic effect with Vincristine
[0036] A fixed concentration of EGCG was administered in combination with Vincristine in nine tumor cell lines. The following King's formula Q value was used to evaluate the combination effect.
[0037] Q=Ea+b / (Ea+Eb-Ea×Eb)
[0038] Among them, Ea+b is the inhibition rate of combined drugs, and Ea and Eb are the inhibition rates of drug A and drug B respectively;
[0039] The numerator represents the actual measured combined inhibition rate of the two drugs, and the denominator is the expected combined inhibition rate.
[0040] The range of Q=0.85-1.15 means simple addition, the range of Q=1.15-2 means enhancement, Q>2 means obvious enhancement, Q<0.55-0.85 means antagonistic effect, and Q<0.55 means obvious antagonism.
[0041] The results show that EGCG and Vincristine have a certain synergistic effect in specific cell lines, indicating that EGCG can enhance the anti-tumor activity of existing anti-live...
Embodiment 3
[0046] Example 3 EGCG can synergize Vincristine and reduce the dosage of Vincristine
[0047] In SK-Hep1 cells, 0.25, 0.5, 0.75 μM Vincristine was combined with 10, 20, 30, 40, 50 μM EGCG, respectively, and 0.25, 0.5, 0.75 μM Vincristine was combined with different concentrations of EGCG to achieve Even exceeded the effect of 1 μM Vincristine alone. According to the statistical analysis of the software, the amount of Vincristine required to achieve half the inhibitory effect when used in combination with EGCG decreases with the increase of the amount of EGCG, and has a concentration gradient effect. The above experimental results show that the combined use of EGCG and Vincristine can effectively enhance the anti-tumor effect of Vincristine and reduce its dosage.
[0048]
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