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Method for extracting total flavone and esculetin from Chinese violet

A technology of violetidine and esculin is applied in the directions of pharmaceutical formulations, non-central analgesics, medical preparations containing active ingredients, etc. High, simple process effect

Inactive Publication Date: 2012-07-04
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, "Research on the Water-Soluble Chemical Components of Viola Viola" and "Research on the Chemical Components of Viola Viola", both documents are extracted by soaking in alcohol, distilled under reduced pressure, dissolved in hot water, and the suspension is washed with acetic acid Ethyl is extracted to obtain the ethyl acetate part, which is purified by silica gel column chromatography. This type of method is not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take Viola dichotoma raw material, take 1kg, add 12kg of 50% ethanol to reflux and extract twice, extract for 3 hours each time, filter to obtain extract, recover ethanol under reduced pressure, add HZ816 (resin 500ml) macropores after diluting the concentrated solution with water Resin column adsorption, after the adsorption is completed, first use 2.5L 20% ethanol solution to elute the impurities, then use 2L 70% ethanol solution to elute the active ingredients, collect the eluent to recover ethanol under reduced pressure, adjust the pH3 with 5% hydrochloric acid solution while it is hot, and place Precipitation, the precipitate was filtered out and dissolved with 200ml of 40% ethanol solution under reflux, filtered, and the solid was dried to obtain 22g of total flavonoids with a content of 87.6%. The filtrate was concentrated under reduced pressure to 80ml and left to crystallize, the crystals were filtered out, dissolved and filtered with ethyl acetate reflux, place...

Embodiment 2

[0022] Take Viola dichotoma raw material, take 1kg, add 10kg of 80% ethanol to reflux and extract twice, extract for 2 hours each time, filter to obtain the extract, recover ethanol under reduced pressure, add SPD300 (resin 500ml) to the large pores after diluting the concentrated solution with water Resin column adsorption, after the adsorption is completed, first use 1.5L 30% ethanol solution to elute the impurities, then use 2.5L 60% ethanol solution to elute the active ingredients, collect the eluent to recover ethanol under reduced pressure, adjust the pH to 5 with 10% hydrochloric acid solution while it is hot, Place the precipitate, filter the precipitate and dissolve it with 300 ml of 20% ethanol solution under reflux, filter, and dry the solid to obtain 20 g of total flavonoids with a content of 90.6%. The filtrate was concentrated under reduced pressure to 150ml and left to crystallize, the crystals were filtered out, dissolved and filtered with ethyl acetate reflux, ...

Embodiment 3

[0024] Crush the raw material of viola radix, take 1 kg, add 8 kg of 60% methanol to reflux extraction for 3 times, extract for 1 hour each time, filter to obtain the extract, recover ethanol under reduced pressure, add ADS-17 (resin 500 ml) to the concentrated solution after diluting with water Macroporous resin column adsorption, after the adsorption is completed, first use 1.5L 30% methanol solution to elute the impurities, then use 2.5L 60% methanol solution to elute the active ingredients, collect the eluent to recover methanol under reduced pressure, and adjust it with 10% hydrochloric acid solution while it is hot pH 4, left to precipitate, the precipitate was filtered out and dissolved with 200ml of 40% ethanol solution under reflux, filtered, and the solid was dried to obtain 21g of total flavonoids with a content of 89.6%. The filtrate was concentrated under reduced pressure to 90ml and left to crystallize, the crystals were filtered out, dissolved and filtered with e...

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PUM

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Abstract

The invention provides a method for extracting total flavone and esculetin from Chinese violet. The method comprises the following steps of: taking a Chinese violet raw material; crushing; adding 50-80% alcoholic solution of which the quantity is 7-15 times of that of the powder for refluxing extraction for 2-3 times; filtering; carrying out concentration under the reduced pressure; adding hot water into the concentrated solution for diluting, and then adding macroporous resin for column adsorption; carrying out gradient elution on the alcoholic solution; carrying out concentration on the eluent under the reduced pressure; adjusting the pH value of the hydrochloric acid solution to be 3-5, and standing for precipitating; filtering out the precipitates; refluxing, dissolving and filtering with 20-40% ethanol solution; drying solid matters to obtain total flavone products; concentrating the filtrate into a small volume, and standing and crystallizing; refluxing, dissolving and recrystallizing with ethyl acetate; and drying the crystals to obtain esculetin products. The method provided by the invention has the advantages of high utilization ratio of raw materials and high degree of industrialization.

Description

Technical field: [0001] The invention belongs to the field of separation of natural products, and in particular relates to a method for extracting total flavonoids and esculin from Viola chinensis. Background technique: [0002] Viola didin is a Viola family plant, which has the effect of clearing heat and detoxifying, cooling blood and reducing swelling. It is commonly used in traditional Chinese medicine to treat boils, carbuncles, scrofula, jaundice, dysentery, diarrhea, red eyes, sore throat, and snake bites. The whole herb of Viola chinensis contains glycosides, flavonoids, esters and other components. Among them, flavonoids and escin are the main functional components. [0003] The total flavonoids of Viola chinensis have antibacterial, anti-inflammatory and antioxidant effects. Li Dinggang et al. used ultrasonic extraction and silica gel column chromatography to separate and purify flavonoids with a purity of 97.5%. The compound has good inhibitory effect on Salmon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/86C07D311/16A61P29/00A61P31/04A61P39/06
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司