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Glycoside ester compound and preparation method and application thereof

A compound and glycoside ester technology, applied in the field of plant active ingredients, can solve the problems of less research on glycoside ester compounds, and achieve good development prospects, good anti-tumor effect, and easy operation

Inactive Publication Date: 2012-07-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many studies on the related chemical components in purslane, but there are few studies on the glycoside ester compounds.

Method used

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  • Glycoside ester compound and preparation method and application thereof
  • Glycoside ester compound and preparation method and application thereof
  • Glycoside ester compound and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 The heavy metal stress of purslane

[0031] Cultivate 100 plants of purslane, divided into 50 plants in the experimental group and 50 plants in the control group; when the purslane emerges, use 2% CuCl 2 The solution was sprayed on the plants for 48 hours; the purslane was harvested after growing to an adult plant, and dried at 60°C.

Embodiment 2

[0032] The preparation of embodiment 2 glycoside ester compound

[0033] Take 500g of dried purslane, soak it in methanol for 2 weeks, suspend the soaking solution in 1L distilled water after concentration, extract the water suspension with 1L n-butanol, and concentrate the n-butanol extract to obtain the extract; (100 mesh, 100g) mixed sample, carry out normal phase silica gel column chromatography separation (200-300 mesh, 1kg; Silica gel column size L 500mm, ), followed by gradient elution with chloroform / methanol with a volume ratio of 9:1, 5:1, 1:1, 1:3, and 1:9, 5 L each time; Fractions were combined and recrystallized from methanol.

Embodiment 3

[0034] The preparation of embodiment 3 glycoside ester compound

[0035] Get 500g dried purslane, extract with 5L ethanol reflux, extract concentrated, water suspension extract with 1L n-butanol, n-butanol extract concentrate; Mix sample with silica gel (100 mesh, 100g), carry out normal phase silica gel column chromatography (200-300 mesh, 1kg; silica gel column size L 500mm, ), followed by gradient elution with a volume ratio of 9:1, 5:1, 1:1, 1:3, 1:9 chloroform / methanol mixture, 5L each time; The fractions of 1 were combined and recrystallized from methanol.

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PUM

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Abstract

The invention discloses a glycoside ester compound and a preparation method and application thereof. The molecular formula of the glycoside ester compound is C26H38O12. The preparation method comprises planting portulaca oleracea linn, and performing heavy metal stress treatment to plants in the growing process of the portulaca oleracea linn.; harvesting after the portulaca oleracea linn grows to an adult plant, placing the whole portulaca oleracea linn plant or the tissues of the portulaca oleracea linn in an organic solvent to perform digestion, separating to obtain digestion liquid, extracting the digestion liquid, and obtaining an extract after the extraction liquid is concentrated; and carrying out chromatographic separation and purification treatment to the extract, and obtaining the glycoside ester compound. The glycoside ester compound with a novel structure is extracted and separated from the planting portulaca oleracea linn undergoing heavy metal stress treatment, has good anti-tumor activity, can be used for preparing anti-tumor drugs or tumor-preventing health-care food, and has good developing prospects.

Description

technical field [0001] The invention relates to the field of plant active ingredients, in particular to a glycoside ester compound and its preparation method and application. Background technique [0002] Purslane (Portulaca oleracea Linn.) is a plant of Amaranthaceae, which is commonly used as food and traditional Chinese medicine, and can be used for the treatment of many diseases ((a) Chan, K.; Islam, M.W.; Kamil, M.; Radhakrishnan, R. ; Zakaria, M.N.M.; Habibullah, M., Attas, A.J. Ethnopharmacol.2000, 73, 445-451. (b) Amin, E.S.; El-Deeb, S.M.Carbohydrate Res. , J.; Sugumaran, M.; Lee, K.L.W. 2004. Herbs of Malaysia: An Introduction to the medicinal, culinary, aromatic and cosmetic use of herbs. Kuala Lumpur: Times Edition.). [0003] Simopoulos et al. isolated omega-3 fatty acids from this plant in 2004 (Simopoulos, A.P. Biol. Res. 2004, 37, 263-277.). Radhakrishnan et al systematically studied the chemical constituents of purslane in 2001, and isolated gallotannins, ...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H1/08A61K31/7032A61P35/00A23L1/29A23L33/00
Inventor 吴斌
Owner ZHEJIANG UNIV
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