Method for continuously extracting and separating sea buckthorn oil and isorhamnetin from sea buckthorn pulps

A technology of isorhamnetin and seabuckthorn oil, which is applied in the direction of fat oil/fat production, fat production, organic chemistry, etc., can solve the problems of difficult mass production, large equipment investment, and long process time, and achieve safe use of reagents and production The effect of strong operability and simple process flow

Inactive Publication Date: 2012-07-04
GUILIN XINGDA PHARMA FACTORY
View PDF6 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No. 02114599.7 used solvent multi-stage extraction to carry out joint extraction of seabuckthorn oil and seabuckthorn flavonoids, but did not specify the purity of the extracted flavonoids, and only extracted flavonoids crude mixture without the process content of isorhamnetin; No. 200610124371.6 extracted Seabuckthorn oil and seabuckthorn flavonoids are the most important; ZL200710139224.0 uses supercritical extraction to degrease, and then uses ethanol to extract crystals. Although its isorhamnetin content can reach 80% to 95%, there is a large investment in supercritical extraction equipment. Time-consuming, difficult to operate and other issues, it is difficult to put into actual mass production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Take 100 kg of seabuckthorn fruit and pulp residue, add 2 t of ethanol with a mass fraction of 95%, and heat and reflux at 70°C for 2 hours;

[0019] (2) Filter the ethanol extract from the previous step, discard the residue, and concentrate the filtrate to extract in vacuo. When concentrating, the vacuum degree is -0.06 MPa to -0.08 MPa, and the temperature is 45°C to 60°C;

[0020] (3) Extract the extract from the previous step with 10L of chloroform, recover the chloroform from the extract to obtain Seabuckthorn oil; heat the insoluble matter in 10L of ethanol with a mass fraction of 95%, dissolve it, and crystallize at room temperature to obtain 1.0 kg of yellow-brown Isochus exosmus Li Su granular crystal, the purity is 70%;

[0021] (4) Take 1.0 kg yellow-brown isorhamnetin granular coarse crystals and add 50 L of ethanol: ethyl acetate (1:1) mixed solvent to heat and dissolve. After standing at room temperature, recrystallize 3 times to obtain 0.5 kg isorhamn...

Embodiment 2

[0023] (1) Take 100 kg of seabuckthorn pulp residue, add 2 t of ethanol with a mass fraction of 95%, and heat and reflux at 70°C for 2 hours;

[0024] (2) Filter the ethanol extract from the previous step, discard the residue, and concentrate the filtrate to extract in vacuo. When concentrating, the vacuum degree is -0.06 MPa to -0.08 MPa, and the temperature is 45°C to 60°C;

[0025] (3) Add 10L of dichloromethane to the extract for extraction, and recover the dichloromethane from the extract to obtain seabuckthorn oil; the insoluble matter is heated and dissolved in 10L of methanol with a mass fraction of 100%, and crystallized at room temperature to obtain 0.8 kg of yellow-brown iso Rhamnetin granular crystals with a purity of 60%;

[0026] (4) Add 50L of ethanol:ethyl acetate (1:1) mixed solvent to 0.8kg of crude crystals and heat to dissolve, then stand at room temperature and recrystallize more than twice to obtain 0.4 kg of isorhamnetin yellow granular crystals with a p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A method for continuously extracting and separating sea buckthorn oil and isorhamnetin from sea buckthorn pulps relates to medicines and includes: (1) taking sea buckthorn fruit and sea buckthorn pulps as raw materials, adding the raw materials into ethanol solution with ratio of feed liquid mass and volume as 1:20, and heating and extracting; (2) filtering extracted solution and concentrating filtrate in vacuum mode into concrete; (3) adopting organic solvents to extract and separate sea buckthorn oil, adopting ethanol or methanol to dissolve not dissolved concrete, and standing for crystallizing at room temperature to obtain yellow brown isorhamnetin granular crystals; and (4) heating and dissolving coarse crystals through a mixed solvent of ethanol and ethyl acetate, and re-crystallizing after standing at room temperature to obtain yellow isorhamnetin granular crystals. The method can be used for continuously extracting and separating sea buckthorn oil and isorhamnetin with 95% purity form the sea buckthorn pulps, has the advantages of being simple in process flow path, stable in product quality, safe in used reagents, strong in industrialized production operability and the like, and solves problems of high device cost, complex processes, cumbersomeness in operation and the like.

Description

technical field [0001] The invention relates to medicines, in particular to a method for continuously extracting and separating seabuckthorn oil and isorhamnetin from seabuckthorn pulp. Background technique [0002] sea ​​buckthorn ( hippophae rhamnoides ), also known as vinegar willow, sour thorn, is a plant of the genus Sourthorn of the family Eupaceae, a deciduous shrub, mainly distributed in Eurasia, and the three northern regions of my country are the main producing areas. my country is rich in seabuckthorn resources, accounting for 95% of the world's total planting area of ​​seabuckthorn. Seabuckthorn juice is rich in vitamin C. The seeds account for about 10% of the whole fruit weight, and the oil content is 7-11%. In recent years, a large number of studies have revealed that seabuckthorn seed oil is effective in antibacterial, enhancing human immunity, anticancer, antiradiation, and anti-inflammatory , promotion of tissue regeneration, treatment of cardiovascula...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C07D311/30C07D311/40
Inventor 何飞杨明武陈伟王黔阳黄五久
Owner GUILIN XINGDA PHARMA FACTORY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap