Method for continuously extracting and separating sea buckthorn oil and isorhamnetin from sea buckthorn pulps

Abstract

A method for continuously extracting and separating sea buckthorn oil and isorhamnetin from sea buckthorn pulps relates to medicines and includes: (1) taking sea buckthorn fruit and sea buckthorn pulps as raw materials, adding the raw materials into ethanol solution with ratio of feed liquid mass and volume as 1:20, and heating and extracting; (2) filtering extracted solution and concentrating filtrate in vacuum mode into concrete; (3) adopting organic solvents to extract and separate sea buckthorn oil, adopting ethanol or methanol to dissolve not dissolved concrete, and standing for crystallizing at room temperature to obtain yellow brown isorhamnetin granular crystals; and (4) heating and dissolving coarse crystals through a mixed solvent of ethanol and ethyl acetate, and re-crystallizing after standing at room temperature to obtain yellow isorhamnetin granular crystals. The method can be used for continuously extracting and separating sea buckthorn oil and isorhamnetin with 95% purity form the sea buckthorn pulps, has the advantages of being simple in process flow path, stable in product quality, safe in used reagents, strong in industrialized production operability and the like, and solves problems of high device cost, complex processes, cumbersomeness in operation and the like.

Description

technical field [0001] The invention relates to medicines, in particular to a method for continuously extracting and separating seabuckthorn oil and isorhamnetin from seabuckthorn pulp. Background technique [0002] sea ​​buckthorn ( hippophae rhamnoides ), also known as vinegar willow, sour thorn, is a plant of the genus Sourthorn of the family Eupaceae, a deciduous shrub, mainly distributed in Eurasia, and the three northern regions of my country are the main producing areas. my country is rich in seabuckthorn resources, accounting for 95% of the world's total planting area of ​​seabuckthorn. Seabuckthorn juice is rich in vitamin C. The seeds account for about 10% of the whole fruit weight, and the oil content is 7-11%. In recent years, a large number of studies have revealed that seabuckthorn seed oil is effective in antibacterial, enhancing human immunity, anticancer, antiradiation, and anti-inflammatory , promotion of tissue regeneration, treatment of cardiovascula...

AI Technical Summary

Problems solved by technology

No. 02114599.7 used solvent multi-stage extraction to carry out joint extraction of seabuckthorn oil and seabuckthorn flavonoids, but did not specify the purity of the extracted flavonoids, and only extracted flavonoids crude mixture without the process content of isorhamnetin; No. 200610124371.6 extracted Seabuckthorn oil and seabuckthorn flavonoids are the most important; ZL200710139224.0 uses supercritical extraction to degrease, and then uses ethanol to extract crystals. Although its isorhamnetin content can reach 80% to 95%, there is a large investment in supercritical extraction equipment. Time-consuming, difficult to operate and other issues, it is difficult to put into actual mass production