Composite nano material modified screen print electrode and method for detecting Fumonisin B1

A technology of screen printing electrodes and fumonisins, which is applied in the field of detection, can solve the problems of unfavorable conventional detection, limited sensitivity, unsuitable for on-site detection, etc., and achieve the effect of easy promotion and application, high sensitivity and high accuracy

Active Publication Date: 2012-07-04
SHANGHAI JIAO TONG UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

In addition, this method requires strict pretreatment of the test samples, high-performance liquid chromatography and other expensive instruments, and requires professional operators, which is not condu

Method used

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  • Composite nano material modified screen print electrode and method for detecting Fumonisin B1
  • Composite nano material modified screen print electrode and method for detecting Fumonisin B1

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Embodiment

[0032] The schematic diagram of the embodiment of the present invention is as figure 1 As shown, the screen printing electrode includes a reference electrode 1, a working electrode 2, and a counter electrode 3; the working area of ​​the working electrode 2 is coated with a multi-walled carbon nanotube 5-colloidal gold 4-chitosan mixture film Layer; when using the screen printing electrode to detect fumonisin B1 (FB1) in the sample, first add OVA-FB16 dropwise on the working electrode that has been coated with the mixture film layer, and then add anti-FB1 monoclonal The mixed solution of Antibody 7 and the sample extract to be tested, after drying, add HRP-labeled goat anti-mouse secondary antibody 8, and finally add the substrate solution H 2 0 2 and HQ.

[0033] The specific steps of the method for preparing the composite nanomaterial modified screen-printed electrode of the present invention and using it to detect fumonisin B1 are as follows:

[0034] Step 1, activation of ...

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Abstract

The invention discloses a composite nano material modified screen print electrode and a method for detecting Fumonisin B1. A working electrode of the screen print electrode is coated with a multi-walled carbon nano tube-colloid gold-chitosan mixture film. The preparation process includes the following steps; activating the multi-walled carbon nano tube; preparing the colloid gold; uniformly mixing the multi-walled carbon nano tube and the colloid gold through the chitosan; coating the mixture on the surface of the working electrode; and completing the preparation after the working electrode is dried at 37 DEG C and the film is formed. The method for detecting Fumonisin B1 provided by the invention is realized in the way that if FB1 exists in a to-be-detected sample, the FB1 competes with FB1 antibodies in the combination with FB1-OVA, so that the antibody amount combined with the FB1-OVA on the electrode is reduced, the amount of HRP-goat-anti-mouse second antibodies on the electrode is reduced, and the current variation value delta I is influenced; and then the content of FB1 in the to-be-detected sample of grains can be judged as per the standard curve drawn through an FB1 standard product. According to the invention, the detection object is single, the pertinence is strong, the accuracy is high, and the sensitivity is strong.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a screen-printed electrode modified with composite nanomaterials (carbon nanotubes and colloidal gold) and a method for detecting fumonisin B1. Background technique [0002] Fumonisin B1 (Fumonisin B1, FB1) is a secondary metabolite produced by Fusarium moniliforme under certain humidity and temperature conditions, and it is widely distributed all over the world. In 1988, Gelderblom et al first isolated fumonisins from the culture solution of Fusarium moniliforme. Fumonisins can contaminate corn and its products, and have been detected in some grain-based products such as noodles, beer, condiments, and even in asparagus. Fumonisins have been reported to be associated with equine leukomalacia, pulmonary edema syndrome in pigs, liver cancer in rats and other diseases. In addition to the above-mentioned diseases, studies in the Transkei region of South Africa and the...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N33/577G01N27/26
Inventor 严亚贤王元凯孙建和王斌秦易
Owner SHANGHAI JIAO TONG UNIV
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