Method for detecting dicyclohexyl phthalate in environmental water sample

A technology of dicyclohexyl phthalate and environment detection, applied in the field of analysis, can solve the problems of time-consuming operation, expensive analysis cost, complicated process and the like

Inactive Publication Date: 2012-07-04
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can separate a variety of phthalates well, the focus of chromatographic technology is the pretreatment of environmental samples, which is complicated, time-consuming, and expensive to analyze.

Method used

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  • Method for detecting dicyclohexyl phthalate in environmental water sample
  • Method for detecting dicyclohexyl phthalate in environmental water sample
  • Method for detecting dicyclohexyl phthalate in environmental water sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The establishment of the standard curve of dicyclohexyl phthalate:

[0069] (1) Coating steps:

[0070] Coat the 96-well ELISA plate with the coating antigen OVA-DCHP at a concentration of 7 μg / mL, 100 μL per well, place in an incubator for 2 hours at 37°C, take out the ELISA plate, pour out the solution in the well, and add Fill it with washing liquid, let it stand for 3-5 minutes, and then spin it dry, a total of three times.

[0071] (2) Closed steps:

[0072] Then add 1% OVA solution, 150 μL per well, incubate at 37° C. for 30 min, block the excess part not coated with antigen, take it out and wash 3 times as above.

[0073] (3) Competition steps:

[0074] Add 50 μL dicyclohexyl phthalate standard solution and 50 μL dicyclohexyl phthalate enzyme-labeled antibody to each well, and incubate at 37°C for 3 hours, so that the substance to be tested and the solid-phase antigen compete for the enzyme label at the same time. The binding site on the antibody. Then wash ...

Embodiment 2

[0079] The reagents used in this embodiment are prepared according to the following formula:

[0080] The water samples for determination were collected from the Yangtze River water in Wuhu section, Jinghu water in Wuhu City, and tap water and mineral water in this laboratory. For the collection of surface water, glass bottles were used to hold the samples, and the bottles were rinsed with the water to be sampled before filling the bottles. 3 times. After the water sample was collected, it was filtered and extracted first, and then the pH value was adjusted to about 7.0 with 1mol / mL HCl or 1mol / mL NaOH, and stored at 4°C until use.

[0081] (1) Coating steps:

[0082] Coat the 96-well ELISA plate with the coating antigen OVA-DCHP at a concentration of 7 μg / mL, 100 μL per well, place in an incubator for 2 hours at 37°C, take out the ELISA plate, pour out the solution in the well, and add Fill it with washing liquid, let it stand for 3-5 minutes, and then spin it dry, a total ...

Embodiment 3

[0091] Except for the competition step: add 25 μL water sample and 25 μL LDCHP standard solution to each well, then add 50 μL dicyclohexyl phthalate enzyme-labeled antibody, incubate at 37°C for 3 hours, and then wash as above. Except, the rest are the same as implementation 2,

[0092] The result processing is also shown in Table 1:

[0093]

[0094]

[0095] ND a : Samples in which DCHP was not detected

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Abstract

The invention discloses a method for detecting dicyclohexyl phthalate in an environmental water sample, and the method comprises the following steps: a, an establishment step of a standard curve, and b, a detection step of the sample. Compared with the prior art, the method not only has the selectivity of an antigen and an antibody of the dicyclohexyl phthalate, but also a measured signal can be amplified by an enzymatic reaction, the sensitivity is improved, and simultaneously, a horse radish peroxidase is ordinary on the market, is cheap and is suitable for popularization. Therefore, by using such an analyzing method with the advantages of simpleness, quickness, low cost, strong specificity and the like, the convenience is provided for the micro monitoring of a dicyclohexyl phthalate pollutant in a proper environment.

Description

technical field [0001] The invention relates to an analysis method, in particular to a simple, rapid and selective detection and analysis method for dicyclohexyl phthalate in environmental water samples. Background technique [0002] Phthalates (also known as phthalates) are a class of compounds in environmental hormones. It is mainly used as a plasticizer, that is, as an additive of plastics to increase the plasticity and strength of plastics, and is also used in the production of coatings, printing and dyeing, cosmetics, perfumes and food packaging materials. In plastics, phthalates act as "lubricant" between molecules to improve the flexibility of the polymer; in cosmetics and fragrances, they can prolong the retention time of fragrance. In recent years, with the use of industrial production and plastic products, plastic waste has increased a lot. Due to the non-chemical reaction between phthalates and polyolefin plastic molecules, they are connected by hydrogen bonds or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/531
Inventor 张明翠王悦
Owner ANHUI NORMAL UNIV
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