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Preparation method of water-soluble soybean polysaccharide

A soybean polysaccharide and water-soluble technology, which is applied in the field of microwave-ultrasonic collaborative technology to extract water-soluble soybean polysaccharides, can solve the problems of low extraction rate, long time, high energy consumption, etc., and achieve the effect of easier leaching and improved extraction rate

Inactive Publication Date: 2013-12-25
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to propose a fast and efficient preparation method for water-soluble soybean polysaccharides in order to overcome the shortcomings of long time, high energy consumption and low extraction rate in the traditional extraction process of water-soluble soybean polysaccharides, and this method is easy to operate and easy to extract High efficiency, low energy consumption, no pollution, easy to realize industrialization

Method used

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  • Preparation method of water-soluble soybean polysaccharide

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Effect test

Embodiment 1

[0048] A kind of preparation method of water-soluble soybean polysaccharide specifically comprises the following steps:

[0049] (1) Add 1L of water to 100g of low-temperature soybean meal, stir and mix well, adjust the pH to 8.50 with 1M sodium hydroxide, control the extraction temperature to 55°C, and the time to 42.8min. After the extraction is completed, control the speed to 4000r / min. After centrifuging for 20 minutes, the supernatant was precipitated to obtain the soybean protein isolate fraction, and the wet residue was retained as a raw material for extracting water-soluble soybean polysaccharides;

[0050] (2) Add 0.3L of water to the above wet residue, stir evenly, adjust the pH of the extract to 2.0 with 1M hydrochloric acid, and obtain a reaction solution;

[0051] (3) Place the reaction solution obtained in step (2) in a microwave-ultrasonic assisted extractor, set the microwave extraction power to 275w, the ultrasonic extraction power to 160w, and set the extract...

Embodiment 2

[0059] A kind of preparation method of water-soluble soybean polysaccharide specifically comprises the following steps:

[0060] (1) Add 3L of water to 300g of low-temperature soybean meal raw material and stir evenly, adjust the pH to 8.50 with 1M sodium hydroxide, control the extraction temperature to 55°C, and the extraction time to 42.8min. After the extraction is completed, control the rotation speed to 4000r / min , the time is 20min to carry out centrifugation, the supernatant is precipitated to obtain the soybean protein isolate component, and the wet residue is retained as the raw material for extracting the water-soluble soybean polysaccharide;

[0061] (2) Add 1.5 L of water to the wet residue obtained in step (1), stir evenly, and adjust the pH to 6.8 with 1M hydrochloric acid to obtain a reaction solution;

[0062] (3) Place the reaction solution obtained in step (2) in a microwave-ultrasonic assisted extractor, set the microwave extraction power to 275w, the ultras...

Embodiment 3

[0068] A kind of preparation method of water-soluble soybean polysaccharide specifically comprises the following steps:

[0069] (1) Add 2L of water to 200g of low-temperature soybean meal raw material and stir evenly, adjust the pH to 8.50 with 1M sodium hydroxide, control the extraction temperature to 55°C, and the time to 42.8min. After the extraction is completed, control the rotation speed to 4000r / min , centrifuged for 20 minutes, and the supernatant was precipitated to obtain the soybean protein isolate component, and the wet residue was retained as the raw material for extracting the water-soluble soybean polysaccharide;

[0070] (2) Add 1.2L of water to the wet residue obtained in step (1), stir evenly, and adjust the pH to 7.5 with 1M hydrochloric acid to obtain a reaction solution;

[0071] (3) Place the reaction solution obtained in step (2) in a microwave-ultrasonic assisted extractor, set the microwave extraction power to 275w, the ultrasonic extraction power to ...

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Abstract

The invention discloses a preparation method of water-soluble soybean polysaccharide, which comprises the following steps of: removing the majority of protein components in low-temperature bean pulp, adding water with the ratio of wet residues and water 1: (3-10), adjusting the pH to 2.0-10.0, extracting with a microwave-ultrasonic assisted extraction device, controlling the microwave power to be 275w, the ultrasonic power to be 160w and the time to be 1-30min, carrying out centrifugation after extraction, concentrating supernatant to one fourth of the original volume, washing sequentially with ethanol of 75 percent, ethanol of 95 percent and absolute ethanol, precipitating, standing at the room temperature, carrying out centrifugation, and freeze-drying under vacuum conditions, thereby obtaining the water-soluble soybean polysaccharide with the molecular weight of 1200-2400kDa. In the preparation method of the water-soluble soybean polysaccharide, industrial low-temperature beam pulp is used as the raw material, the energy consumption for extraction process is low, the extraction time can be reduced significantly, and the extraction rate can be increased. Therefore, the method is an environmentally friendly method for extraction of the water-soluble soybean polysaccharide.

Description

technical field [0001] The invention relates to a polysaccharide extraction process, in particular to a method for extracting water-soluble soybean polysaccharides by using low-temperature soybean meal as a raw material, removing protein, and using microwave-ultrasonic collaborative technology. Background technique [0002] Low-temperature soybean meal is the residue after industrial production of soybean oil, which is rich in polysaccharide components (about 30%) and protein components (about 40%), and the content of water-soluble soybean polysaccharide components is rich, accounting for about 2 / 2 of the polysaccharide components. 3. It is an ideal raw material for extracting water-soluble soybean polysaccharides. [0003] The traditional techniques for extracting water-soluble soybean polysaccharides include: hot water extraction, alkaline extraction, organic acid extraction, complex enzyme extraction, membrane separation, subcritical water extraction, etc. Factors such as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 管骁陈姿含
Owner UNIV OF SHANGHAI FOR SCI & TECH
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