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Culture medium capable of increasing biomass of rhodotorula glutinis and application of culture medium

A technology of rhodotorula viscosus and culture medium, which is applied in the field of medium for increasing the biomass of rhodotorula viscoscens, which can solve problems such as waste of resources, slow cell proliferation rate, and difficulty in adapting to the industrial production of rhodotorula viscoscens, and achieve the effect of reducing costs

Inactive Publication Date: 2012-07-11
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the centrifugal sediment was partially discarded, which not only wastes resources but also increases the cost of cultivation
The common feature of these two media is that the cell proliferation rate is relatively slow, and it is difficult to adapt to the industrial production of Rhodotorula viscosus. Therefore, it is imperative to study a method to increase the biomass of Rhodotorula viscosus, which has important practical significance

Method used

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  • Culture medium capable of increasing biomass of rhodotorula glutinis and application of culture medium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 sticky rhodotorula culture medium preparation

[0032] (1) Dissolve 100g of malt extract powder in 1L of distilled water, centrifuge at 4000r / min for 15min, filter with filter paper, and dilute the supernatant 1×10 5 Pa autoclave for 15 minutes;

[0033] (2) Combine the centrifuged precipitate in step (1) and the precipitate formed during the sterilization process, stir with 4 times the volume of distilled water at a high speed of 2600r / min for 30min, and centrifuge the supernatant at 4000r / min for 20min Combine with the centrifuged supernatant of step (1), adjust the pH value of the solution to 5.0, and then pass through 1×10 5 Pa high-pressure sterilization again for 15 minutes to obtain Rhodotorula viscosus medium.

Embodiment 2

[0034] Embodiment 2 sticky rhodotorula culture medium preparation

[0035] (1) Dissolve 100g of malt extract powder in 1L of distilled water, centrifuge at 3000r / min for 15min, filter with filter paper, and dilute the supernatant 1×10 5 Pa autoclave for 30min;

[0036] (2) Combine the centrifuged sediment in step (1) and the precipitate formed in the sterilization process, use 2 times the volume of distilled water for ultrasonic treatment at 800W for 15 minutes, and centrifuge the supernatant at 3000r / min for 15 minutes with step ( 1) Combine the centrifuged supernatants, adjust the pH value of the solution to 5.5, and then pass through 1×10 5 Pa high-pressure sterilization again for 30 minutes to obtain Rhodotorula viscosus medium.

Embodiment 3

[0037] Embodiment 3 sticky rhodotorula culture medium preparation

[0038] (1) Dissolve 100g of malt extract powder in 1L of distilled water, centrifuge at 6000r / min for 15min, filter with filter paper, and dilute the supernatant 1×10 5 Pa autoclave for 20min;

[0039] (2) Combine the centrifugal sediment in step 1) and the sediment formed in the sterilization process, carry out high-speed 800r / min stirring with 5 times the volume of distilled water for 60min, and centrifuge at 6000r / min for 15min with the supernatant The centrifuged supernatant of step (1) was combined, and the pH value of the solution was adjusted to 5.3, and then subjected to 1×10 5 Pa high-pressure sterilization again for 20 minutes to obtain Rhodotorula viscosus medium.

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Abstract

The invention provides a culture medium capable of increasing biomass of rhodotorula glutinis and a method for fermenting rhodotorula glutinis. The method comprises the following steps: centrifuging a wort culture medium; remaining supernate; re-dissolving the precipitate part; combining the dissolved part with the supernate obtained by centrifugation in the previous step to serve as a culture medium; cultivating under the conditions of the temperature of between 25 and 33 DEG C and the rotating speed of 120 to 160 r / min for 96 to 192 hours; fermenting the rhodotorula glutinis, wherein the result shows that the biomass of the rhodotorula glutinis is increased by 70 to 100 percent compared with that of rhodotorula glutinis treated by the traditional method. The method has high application value.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a medium for increasing the biomass of Rhodotorula viscosus and its application. Background technique [0002] Rhodotorula glutinis is one of oil-producing microorganisms, and its oil content is more than 25%-40%. In recent years, the world is facing the challenges of climate change and energy crisis. Biofuels, including microbial oils, have become one of the research hotspots, and research on Rhodotorula viscosus has also attracted much attention. The amount of oil produced by Rhodotorula viscosus depends on two aspects, one is the oil content of Rhodotorula viscosus, and the other is its biomass. The increase of biomass is the result of rapid cell proliferation of Rhodotorula glutinosa during the fermentation process, indicating that the medium is more suitable for its growth. In the practical application of Rhodotorula viscosus, such as producing oil or extracting phys...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12R1/645
Inventor 李博生王政
Owner BEIJING FORESTRY UNIVERSITY